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Prikl Biokhim Mikrobiol ; 15(4): 612-7, 1979.
Article in Russian | MEDLINE | ID: mdl-42047

ABSTRACT

The methods of extracting total protein from the biomass of Spirulina platensis are discussed. The results of studying different procedures of cell wall disruption, soluble protein extraction and precipitation are presented. The best results can be obtained using mechanical disintegration of Spirulina cells for 20 min (at a temperature not higher than 25 degrees C), soluble protein extraction with 0.4% NaOH, and its subsequent precipitation at the isoelectric point with 5% HCl.


Subject(s)
Bacteria/analysis , Bacterial Proteins/isolation & purification , Evaluation Studies as Topic , Hydrogen-Ion Concentration , Methods , Temperature
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