Evaluation of serum oxidant/antioxidant balance in patients with acute stroke.

OBJECTIVES: To investigate the alterations in the oxidant-antioxidant balance in patients with acute ischaemic stroke, and to locate any correlation between oxidant/antioxidant parameters and the National Institute of Health Stroke Scale. METHODS: The case-control study was conducted at the Neurology Department of Dicle University Medical Faculty, Diyarbakir, Turkey, from June 2010 to June 2011. Blood samples were obtained from 53 patients with ischaemic stroke and 40 healthy controls without any history of ischaemic stroke or systemic disease. Venous blood was obtained within 24 hour after stroke onset. Serum malondialdehyde, total anti-oxidant capacity, paraoxanase and superoxide dismutase were measured. SPSS 11.5 used for statistical analysis. RESULTS: There was no difference between the cases and the controls regarding age [64.5 +/- 15.8 and 66.3 +/- 13.9 respectively], gender [27 (51%) / 26 (49%), and 19 (48%) / 21 (52%) respectively], obesity [15(28.3%) and 13(37.5%), respectively], and hypertension [30 (56.6%) and 23 (57.5%), respectively]. The cases had higher concentrations of malondialdehyde (147.3 +/- 59.3 vs. 112.4 +/- 28.5 nmol/gr protein, p < 0.001), and superoxide dismutase (4.40 +/- 0.79 vs. 3.35 +/- 0.51, p < 0.001) compared to the controls. However, the cases had lower concentrations of paraoxanase (23.2 +/- 23.7 vs 64.7 +/- 52.6, p < 0.001), total anti-oxidant capacity (0.77 +/- 0.38 vs. 0.95 +/- 0.30, p < 0.015), and nitric oxide (10.8 +/- 7.1 vs. 17.5 +/- 2.4 micromol/gr protein, p < 0.001), compared to the controls. In the stroke group, a significant negative correlation was found between the National Institute of Health Stroke Scale and total anti-oxidant capacity activity (p < 0.021, r = -0.32). CONCLUSION: The results support the hypothesis that sufficient anti-oxidant capacity has a beneficial effect on the clinical severity of acute ischaemic stroke.

Oxytocin Ameliorates Remote Liver Injury Induced by Renal Ischemia-Reperfusion in Rats

Renal ischemia-reperfusion (IR) causes remote liver damage. Oxytocin has anti-inflammatory and antioxidant effects. The main purpose of this study was to evaluate the protective function of oxytocin (OT) in remote liver damage triggered by renal IR in rats. Twenty four rats were randomly divided into four different groups, each containing 8 rats. The groups were as follows: (1) Sham operated group; (2) Sham operated+OT group (3) Renal IR group; (4) Renal IR+OT group. OT (500microg/kg) was administered subcutaneously 12 and 24 hours before and immediately after ischemia. At the end of experimental procedure, the rats were sacrificed, and liver specimens were taken for histological assessment or determination of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), paraoxonase (PON-1) activity and nitric oxide (NO). The results showed that renal IR injury constituted a notable elevation in MDA, TOS, Oxidative stress index (OSI) and significantly decreased TAS, PON-1 actvity and NO in liver tissue (p<0.05). Additionally renal IR provoked significant augmentation in hepatic microscopic damage scores. However, alterations in these biochemical and histopathological indices due to IR injury were attenuated by OT treatment (p<0.05). These findings show that OT ameliorates remote liver damage triggered by renal ischemia-reperfusion and this preservation involves suppression of inflammation and regulation of oxidant-antioxidant status.