Assessment of Microgap and Microbial Leakage of Two Different Implant-abutment Interfaces: An In Vitro Study.

AIM: The purpose of the current study was to evaluate Titanium and Bioneck TRI implant-abutment interfaces for microgaps and microbiological leakage. MATERIALS AND METHODS: In this in vitro experiment, 40 dental implants were split into two groups, each of which had 20 samples. Group I: Titanium dental implant, group II: Bioneck TRI. E. coli strain was cultivated in MacConkey media for 24 hours at 37°C. To achieve a bacterial concentration of 1 × 108 colony-forming units per mL at 0.5 scale of MacFarland, the brain-heart infusion (BHI) broth was injected. The CFU count was done to evaluate the microbial leakage. The parts were first submerged, carefully cleaned in an ultrasonic bath, and then installed using a digital torque meter with a 20 N/cm preload. These were attached to a stub of approximately 13 mm using carbon tape, and the microgap evaluation was performed using a scanning electron microscope at a magnification of x1000. Unpaired t-test was used for the calculated data's statistical analysis. The p-value less than 0.05 was considered as statistically significant. RESULTS: The maximum microbial leakage was in Bioneck TRI implants (10000 ± 0.01) followed by Titanium dental implants (8.60 ± 3.16). The mean difference was 9991.40 and there was a statistically significant difference found between the two different groups. The maximum microgap was found in the Bioneck TRI implants (9.72 ± 0.96), followed by Titanium dental implant (6.82 ± 1.10) and there was a statistically significant difference was found between the groups (p < 0.001). CONCLUSION: The present study concluded that the microorganisms can infiltrate the microgap between the implant and abutment interface. When compared with Titanium dental implants, Bioneck TRI implants showed significantly higher levels of microbial leakage. CLINICAL SIGNIFICANCE: A microgap between the implant and abutment connection might operate as a bacterial source, may produce inflammation, even osseointegration in danger, and subsequently alter clinical and histological parameters. Therefore, having an understanding of the compatible components aids in overcoming treatment planning challenges.

Knowledge, Attitudes, and Practices of Dental Healthcare Workers toward Hepatitis B Virus in Khartoum/Sudan.

OBJECTIVE: The aim of the present study is to evaluate dental healthcare workers' knowledge, attitudes, and practices toward hepatitis B infection. MATERIALS AND METHODS: This study was a cross-sectional self-administered, structured questionnaire survey that was conducted in Khartoum/Sudan. The questionnaire was completed by 177 dental healthcare providers who practice in public dental clinics in Khartoum state. The completion rate was 100%. RESULTS: The study participants showed relatively acceptable knowledge of hepatitis B virus (HBV) infection. The majority (98.3%) were familiar with hepatitis B infection. About 93% answered correctly that blood, blood products, and needles/sharps are the route of transmission of HBV. About 65.5% completed HBV vaccination. About 59.3% had a history of needle sticks and only 16% reported their injury. Dentists and nurses almost had the same knowledge, but dentists were slightly having better knowledge in some aspects. Statistical Package for Social Sciences (SPSS) version 20 was used. Chi-square test was used to determine the relationship between categorical variables. CONCLUSION: Most of the study participants were aware of HBV infection, routes of transmission, prevention, and necessity of vaccination, but they are deficient in some areas, like needle-stick injury protocol and post-exposure prophylaxis (PEP). The study revealed a low level of HBV vaccination coverage rate. Further strategies for preventing workplace exposure, training programs on HBV infection, including PEP, and increasing the vaccination coverage rate of all healthcare workers are highly recommended. CLINICAL SIGNIFICANCE: Dental healthcare workers are at high risk of acquiring hepatitis B infection. The majority of exposure in dentistry is preventable. Understanding the knowledge and awareness of dental health toward hepatitis B is crucial to design and apply preventive measures to control transmission and potential complications.