ABSTRACT
A multimode fiber (MMF) is a minimally invasive imaging probe. The most popular approach of MMF-based microscopy is raster-scan imaging, where the sample is illuminated by foci optimized on the fiber output facet by wavefront shaping (WFS). Imaging quality can be quantified by characteristic parameters of the optimized spots. We investigate the influence of the input light position on WFS through a round-core MMF with partial mode control, a situation often encountered in real life. We further demonstrate a trade-off between the shape and contrast of the foci generated on the output facet: the center input position is beneficial for high-contrast imaging, while the edge input position helps to reduce focus aberrations. These results are important for high field-of-view raster-scan imaging via an MMF.
Subject(s)
Diagnostic Imaging , Optical FibersABSTRACT
Aberrations and scattering limit the ability of optical microscopy to penetrate deep tissues. Adaptive optics (AO) is a very effective technique to correct for smooth aberrations. Wavefront shaping (WFS) techniques, on the other hand, compensate both scattering and aberrations and have guaranteed convergence even for finding high-dimensional corrections. However, if it is known in advance that the required corrections should be smooth, WFS is suboptimal because it does not use this a priori information. Here, we combine the best of AO and WFS by introducing a WFS method that takes into account the smoothness of the required correction. Our method is numerically stable and robust against noise, and it can find the corrections for multiple targets simultaneously. We experimentally confirmed that this method outperforms existing WFS techniques, especially in forward scattering samples.
ABSTRACT
The modified Born series method is currently one of the most efficient methods available for simulating light scattering in large inhomogeneous media. However, to achieve high accuracy, the method requires thick gradually absorbing layers around the simulation domain. Here, we introduce new boundary conditions, combining a padding-free acyclic convolution with an ultra-thin boundary layer. Our new boundary conditions minimize the wrap-around and reflection artefacts originating from the edges of the simulation domain, while also greatly reducing the computational costs and the memory requirements of the method. Our GPU-accelerated Matlab implementation is available on GitHub.
ABSTRACT
Wavefront shaping is increasingly being used in modern microscopy to obtain high-resolution images deep inside inhomogeneous media. Wavefront shaping methods typically rely on the presence of a "guide star" to find the optimal wavefront to mitigate the scattering of light. However, the use of guide stars poses severe limitations. Notably, only objects in the close vicinity of the guide star can be imaged. Here, we introduce a guide-star-free wavefront shaping method in which the optimal wavefront is computed using a digital model of the sample. The refractive index model of the sample, that serves as the input for the computation, is constructed in situ by the microscope itself. In a proof of principle imaging experiment, we demonstrate a large improvement in the two-photon fluorescence signal through a diffuse medium, outperforming state-of-the-art wavefront shaping by a factor of two in imaging depth.
ABSTRACT
In this paper, we propose a reconfigurable beam-shaping system to permit energy-efficient non-line-of-sight (NLOS) free-space optical communication. Light is steered around obstacles blocking the direct communication pathway and reaches a receiver after reflecting off of a diffuse surface. A coherent array optical transmitter (CAO-Tx) is used to spatially shape the wavefront of the light incident on a diffuse surface. Wavefront shaping is used to enhance the amount of diffusely reflected light reaching the optical receiver. Synthetic NLOS experiments for a signal reflected over an angular range of 20° are presented. A record-breaking 30-Gbit/s orthogonal frequency-division multiplexing signal is transmitted over a diffused optical wireless link with a >17-dB gain.
ABSTRACT
Scattering prevents light from being focused in turbid media. The effect of scattering can be negated through wavefront shaping techniques when a localized form of feedback is available. Even in the absence of such a localized reporter, wavefront shaping can blindly form a single diffraction-limited focus when the feedback response is nonlinear. We developed and experimentally validated a model that accurately describes the statistics of this blind focusing process. We show that maximizing the nonlinear feedback signal only results in the formation of a focus when a limited number of reporters are contributing to the signal. Using our model, we can calculate the minimal requirements for the number of controlled spatial light modulator segments and the order of nonlinearity to blindly focus light through strongly scattering media.
