ABSTRACT
In this study, we aim to evaluate the chemical characteristics, and nutritional quality of raw meat from broiler chicken fed a black soldier fly larvae meal. Three hundred twenty female broiler chicks were divided into four dietary treatments (8 replicates/treatments). Birds were fed maggot meal at 0%, 4%, 8%, and 12%. At 42 days of age, 16 birds/treatment were randomly selected and slaughtered. The chemical characteristics and nutritional quality of the meat were assessed. Abdominal fat, intramuscular lipid levels, and cholesterol levels increased (p < 0.05) in chicken-fed larvae meal. The latter had a higher level of mono-unsaturated and polyunsaturated fatty acids but a lower level of omega 3 (ω3) compared to the control. The atherogenicity index (AI) and the thrombogenicity index (TI) decreased with maggot meal incorporation. The unsaturation index was better at 4% and 8% maggot meal. Chickens fed with 8% maggot meal would present less risk for cardiovascular health.
ABSTRACT
Reversed-phase ion-pair high-performance liquid chromatography (HPLC) was coupled with detection by UV absorption (280 nm) for separation and quantitation of added folic acid (FA) in fortified cereal based foods. A simple and rapid liquid-solid extraction method, combined with enzymatic digestion, to recover FA from the sample matrices is also presented. The quantitation of added FA was achieved in products including corn (maize), wheat-, rice- and oat-based cereal breakfast foods fortified at 25% and 100% of the reference daily intake (RDI). The retention time for FA was ca. 15 min, and the detection limit was 2 ng/20 microliters injection for standard FA. When FA was added to unfortified samples of wheat flour at concentrations of 3.08 or 20.0 micrograms/g, recoveries were 93% and 96%, respectively. Comparison of HPLC results with those of a standard microbiological assay has shown quite good agreement (r = 0.998). A solid-phase extraction clean-up procedure has also been developed for use with samples fortified with low levels of FA, where interferences may otherwise hinder quantitation.
