ABSTRACT
OBJECTIVES: Genotyping of Salmonella strains is an important molecular tool to discriminate isolates and to improve epidemiological studies when an outbreak occurs. Among the DNA-based genotyping methods, pulsed-field gel electrophoresis (PFGE) is currently used to subtype Salmonella isolates. In this study, the feasibility of genotyping Salmonella enterica serotype Infantis strains using XbaI restriction enzyme was evaluated. Separation of restricted fragments was performed by PFGE. METHODS: To test the possibility of applying this methodology to epidemiological investigation, a collection of 26 Salmonella Infantis strains were tested for their susceptibility to 14 antimicrobial agents and were analysed by XbaI macrorestriction followed by PFGE. Detection of class 1 integrons as well as intI1 and blaTEM genes in resistant strains was also studied. RESULTS: Antimicrobial susceptibility testing showed that 84.6% (22/26) of Salmonella Infantis isolates were susceptible to all of the antimicrobials tested, whereas 7.7% (2/26) had low-level resistance to ß-lactams and harboured the blaTEM gene. A class 1 integron (0.8kb) and the intI1 gene (898bp) were detected in one Salmonella Infantis strain. However, five different PFGE profiles were defined by XbaI macrorestriction. CONCLUSIONS: The PFGE method demonstrated adequate typing ability and represents a powerful tool to discriminate the serotype Salmonella Infantis.
Subject(s)
Drug Resistance, Bacterial , Genotyping Techniques/methods , Salmonella/genetics , Bacterial Typing Techniques , Electrophoresis, Gel, Pulsed-Field , Food Microbiology , Humans , Microbial Sensitivity Tests , Morocco , Salmonella/drug effects , Salmonella/isolation & purification , Salmonella InfectionsABSTRACT
Polymerase chain reaction (PCR) amplification using the AmpFl STR Identifiler kit was performed in a random sample of 204 unrelated individuals from the Arabic-speaking population of the southern Morocco. Allele frequencies of 15 STRs loci (D13S317, D16S539, D2S1338, vWA, TPOX, D18S51, D5S818, FGA, D8S1179, D21S11, D7S820, D19S433, CSF1PO, TH01 and D3S1358) have been reported in this population. Markers D18S51, FGA, D2S1338 and D21S11 had the highest power of discrimination (PD) values while TH01 was the most informative locus in the studied population. The phylogenetic tree established among worldwide populations and genetic distance values show a great affinity between the Southern Moroccan population, Saudian, Moroccan of Asni and Andalusian. Our data is useful for anthropological and other comparative studies of populations and is powerful for forensic and paternity testing in the Arabic-speaking population of the Southern Morocco.
