ABSTRACT
Immune aggression to transplanted allogeneic bone marrow, i.e. the graft-versus-host disease (GVHD), could be decreased by the suppression of effector and/or activation of T- regulatory cells (Treg). This task could be solved by co-transplantaiton of allogeneic bone marrow and mesenchymal stromal cells (MSCs). This study demonstrated the elevated immune modulating activity of MSCs by their culturing in vitro on Al203 oxide nanocoatings. Introduction of the cells to the animals with GVHD resulted in an increased content of Treg in the spleen of bone marrow recipients, reduced severity of the pathology, and higher survival of animals. Thefindings could be the basis for developing the new approaches to optimize the GVHD treatment methods involving the oxide nanocoating cultured MSCs.
Subject(s)
Aluminum Oxide/chemistry , Cell Culture Techniques/methods , Graft vs Host Disease/therapy , Mesenchymal Stem Cell Transplantation/methods , Mesenchymal Stem Cells/cytology , Nanostructures/chemistry , Animals , Disease Models, Animal , Graft vs Host Disease/immunology , Mice, Inbred C57BL , Mice, Inbred CBA , T-Lymphocytes, Regulatory/immunology , Transplantation, HomologousABSTRACT
We studied the effect of nanocomposite coatings with various physicochemical properties on the structural and functional properties (adhesion potential, phenotype, gene expression) of mesenchymal stem cells. Of all tested nanocoatings (Al2O3, ZrO2, Ta2O5), oxide coating Al2O3 enriched in vitro monolayer bone marrow cell culture with cells carrying mesenchymal stem cells phenotype markers and stimulated expression of ido gene, which can confer new therapeutic potencies to these cells and extend their application in clinical practice.
Subject(s)
Cell Adhesion/drug effects , Coated Materials, Biocompatible/pharmacology , Indoleamine-Pyrrole 2,3,-Dioxygenase/biosynthesis , Mesenchymal Stem Cells/drug effects , Nanocomposites/chemistry , Tissue Engineering/methods , Aluminum Oxide/pharmacology , Animals , Antigens, Differentiation/biosynthesis , Bone Marrow Cells/drug effects , Cells, Cultured , Coated Materials, Biocompatible/chemistry , Gene Expression/drug effects , Materials Testing , Mice , Mice, Inbred CBA , Oxides/pharmacology , Surface Properties , Tantalum/pharmacology , Zirconium/pharmacologyABSTRACT
The agar culture method was used to state that colony-forming units responsible for cell structures of a hemopoietic series remained undamaged after cryopreservation (-196 degrees) in a suspension of mouse and human donor bone marrow. Cultivation of cryopreserved bone marrow revealed the inhibition of proliferative activity of cells growing in agar as compared with native ones. The inhibition of DNA synthesis was the basis of this process.
Subject(s)
Bone Marrow Cells , Animals , Bone Marrow/drug effects , Bone Marrow/metabolism , Cell Division/drug effects , Colony-Forming Units Assay , Cryoprotective Agents/pharmacology , DNA/metabolism , Freezing , Humans , Mice , Mice, Inbred BALB C , Polyethylene Glycols/pharmacology , Time FactorsABSTRACT
Investigation carried out by means of chromosome marker T6T6 showed that frozen donor and cadaver bone marrow cells (6 h and 12 h after death) survive and proliferate in the organism of lethally irradiated recipient; the proliferation and differentiation intensity of cadaver cells is reduced in comparison with the donor cells.
Subject(s)
Bone Marrow/radiation effects , Temperature , Tissue Preservation , Animals , Bone Marrow Transplantation , Cadaver , Cell Differentiation/radiation effects , Cell Division/radiation effects , Colony-Forming Units Assay , Hematopoiesis/radiation effects , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Tissue DonorsABSTRACT
The growth-enhancing effect and the differentiation of colonies formed by native and frozen-thawed myelokaryocytes in semiliquid agar were observed over a period from the 2nd to the 28th day. The cultivation of bone marrow in agar was shown to form colonies consisting of haemopoietic cells as well as of stromal elements. Under the cultivation of frozen-thawed bone marrow, the retardation of proliferative activity of growing cells was observed within up to 4 days. Later on the proliferative character and the rate of the myelokaryocytes of both the types were seen identical. The question of a possible existence of haemopoietic tissue and stromal elements in the progenitor cells of normal bone marrow is being discussed.
Subject(s)
Bone Marrow Cells , Agar , Animals , Cell Differentiation , Cell Division , Cells, Cultured , Colony-Forming Units Assay , Culture Media , Humans , Mice , Mice, Inbred CBA , Time FactorsABSTRACT
The character of cell reactions, number of macrophages at the place of skin allograft was demonstrated to depend on the stage of posttransplantational period. Macrophagal reaction increases as the crisis of rejection is approaching; functional and metabolic activity of macrophages is increasing that is evident from an intensive vacuolization of cytoplasm, decreased contents of glycogene, enhanced acid phosphatase activity in them, increased amount of H3-thymidin labelled cells.
