Symbiotic Nitrogen Fixation in Soil Contaminated with the Veterinary Antibiotics Oxytetracycline and Sulfamethazine.

Veterinary and growth-promoting antibiotics are widely used in animal husbandry and accumulate in manure-fertilized soils. However, the impact of these antibiotics on symbiotic nitrogen fixation is poorly understood. We investigated the effect of the veterinary antibiotics oxytetracycline and sulfamethazine, and a combination of both, on nitrogen fixation in alfalfa ( L.) inoculated with . In a pot experiment, was grown in soils fertilized with fresh manure that contained environmentally relevant antibiotic concentrations (0.2, 2, and 200 mg kg). Nodulation, nitrogen fixation, and nutrient concentrations were determined in the alfalfa plants and soils after 12 wk. Compared with the antibiotic-free control, symbiotic nitrogen fixation increased significantly in soils mixed with manure containing 2 and 200 mg kg oxytetracycline (20.1 and 20.8% increase, respectively) and a mixture of 200 mg kg oxytetracycline and sulfamethazine (12.4% increase). The measured plant- and soil-related parameters failed to explain the observed increase in nitrogen fixation. However, using concentration levels that accurately reflect common agricultural practices, we obtained results that directly contradict other experiments conducted under unrealistically high antibiotic concentrations.

Phosphorus mitigation remains critical in water protection: A review and meta-analysis from one of China's most eutrophicated lakes.

The processes of urbanization and industrialization within geological phosphorus-rich mountains (GPMn) have resulted in water degradation within southwest China. Lake Dianchi, one of the most eutrophicated lakes in China, has epitomized this issue. Clear understandings of phosphorus (P) mitigation efforts, the evolution of P budgets, and possible risks in the Dianchi system will benefit future eutrophication control, providing valuable lessons for other plateau freshwater lakes. In this study, we applied systematic review methodology to investigate the above questions, and then compared the results with other lakes worldwide. Generally, meta-analytical approaches have indicated P levels remain a key factor in causing algal blooms. Post-2015, the P budget of the Dianchi system, especially in Caohai section, was modified. However, it's still experiencing high pressures from P enrichment (Caohai: 0.4 mg·l-1; Waihai: 0.2 mg·l-1). The flux of P in Dianchi remains high, both through the external P load (556 ton·a-1), and an internal cycle (304 ton·a-1 associated with the absorption, deposition and removal of algae biomass; and 380 ton·a-1 associated with sediment exchange). Meanwhile, significant P retention has been observed in the lake, in particular within the Waihai section (211 ton·a-1). Currently, water diversion (from external watersheds), sewage diversion, and sediment-dredging projects have benefited Dianchi. However, continuous urbanization and GPMn ecological degradation could introduce hundreds of tons of additional P, leading to subsequent algal blooms. Furthermore, beyond Lake Dianchi, other lakes and reservoirs in southwest China are facing similar issues regarding P mitigation, especially in GPMn regions, though corresponding knowledge is still limited. Therefore, effective and flexible sub-regional protection strategies and research related to external and internal P mitigations have become key requirements for Lake Dianchi management. Meanwhile, ecologically sensitive approaches to GPMn regions, as well as city development within basin and market driven treatments, should be incorporated into regional water source protection for southwest China.

The importance of plot size and the number of sampling seasons on capturing macrofungal species richness.

The species-area relationship is an important factor in the study of species diversity, conservation biology, and landscape ecology. A deeper understanding of this relationship is necessary, in order to provide recommendations on how to improve the quality of data collection on macrofungal diversity in different land use systems in future studies, a systematic assessment of methodological parameters, in particular optimal plot sizes. The species-area relationship of macrofungi in tropical and temperate climatic zones and four different land use systems were investigated by determining the macrofungal species richness in plot sizes ranging from 100 m2 to 10 000 m2 over two sampling seasons. We found that the effect of plot size on recorded species richness significantly differed between land use systems with the exception of monoculture systems. For both climate zones, land use system needs to be considered when determining optimal plot size. Using an optimal plot size was more important than temporal replication (over two sampling seasons) in accurately recording species richness.

The meaning of employment from the perspective of persons with spinal cord injuries in six European countries.

BACKGROUND: People with spinal cord injuries (SCI) are underrepresented in the labour force. OBJECTIVE: To examine the meaning of employment, as it is understood in the context of participation and integration in society, among persons with SCI in six European countries. We ask how SCI relates to employment, for the functions of employment, alternatives to employment, and its obstacles. METHOD: Semi-structured interviews were conducted with 74 persons and qualitatively analysed using a grounded theory approach. RESULTS: Employment was ranked as very important independent of the participants current employment status. We identified three main functions of employment: 1) employment contributes to the creation of personal and collective identity and social recognition; 2) employment enables structuring of time and distracts from impairment and pain; 3) employment is as an important social arena that facilitates interaction with other people. Voluntary work and domestic work did not fully replace the social functions of employment, nor correspond to the design of the disability compensation systems. CONCLUSION: This study illustrates the high value of employment and a need to pay more attention to a broader range of productive work. It also reveals the interdependencies between employment status and income mediated by the disability compensation schemes.

Tracing copper derived from pig manure in calcareous soils and soil leachates by 65Cu labeling.

Copper is used as a growth promoter in animal husbandry, resulting in high Cu concentrations in animal manure. We tested whether Cu would be mobilized in soils receiving excessive loads of manure, both from recently added and from aged fractions. To discriminate between these Cu sources, manure was labeled with (65)Cu. After soil application of 0, 15, and 30 Mg manure ha(-1), leachate was collected in free-draining lysimeters (40 cm depth) under undisturbed soil over a 53 day period. Determining the total amounts of Cu and the fractions of (65)Cu in leachate and the soil profile enabled us to trace the translocation of Cu derived from labeled manure. More than 84% of the applied Cu was retained in the top 2 cm of soil. Less than 0.01% of the applied Cu was detected overall in the leachate. Of this amount, however, 38% (± 8.9 SE) was leached within 8 days after application. The total Cu concentration in leachates (32-164 µg L(-1)) frequently exceeded the Chinese groundwater quality standard of 50 µg L(-1). The added (65)Cu, however, accounted for less than 3.6% of the total Cu leaching load, suggesting that Cu from older sources and/or geological background controls contamination, regardless of current land management.

Identification of soil contamination hotspots with veterinary antibiotics using heavy metal concentrations and leaching data--a field study in China.

In regions with high livestock densities, the usage of antibiotics and metals for veterinary purposes or as growth promoters poses a risk in manured soils. We investigated to which degree the concentrations and depth distributions of Cu, Zn, Cr and As could be used as a tracer to discover contaminations with sulfonamides, tetracyclines and fluoroquinolones. Besides, we estimated the potential vertical translocation of antibiotics and compared the results to measured data. In the peri-urban region of Beijing, China, soil was sampled from agricultural fields and a dry riverbed contaminated by organic waste disposal. The antibiotic concentrations reached 110 µg kg(-1) sulfamethazine, 111 µg kg(-1) chlortetracycline and 62 µg kg(-1) enrofloxacin in the topsoil of agricultural fields. Intriguingly, total concentrations of Cu, Zn, Cr and As were smaller than 65, 130, 36 and 10 mg kg(-1) in surface soil, respectively, therewith fulfilling Chinese quality standards. Correlations between sulfamethazine concentrations and Cu or Zn suggest that in regions with high manure applications, one might use the frequently existing monitoring data for metals to identify potential pollution hotspots for antibiotics in topsoils. In the subsoils, we found sulfamethazine down to ≥2 m depth on agricultural sites and down to ≥4 m depth in the riverbed. As no translocation of metals was observed, subsoil antibiotic contamination could not be predicted from metal data. Nevertheless, sulfonamide stocks in the subsoil could be estimated with an accuracy of 35-200 % from fertilisation data and potential leaching rates. While this may not be sufficient for precise prediction of antibiotic exposure, it may very well be useful for the pre-identification of risk hotspots for subsequent in-depth assessment studies.

Leaching of veterinary antibiotics in calcareous Chinese croplands.

Veterinary antibiotics reach the soil environment by manure application, where they accumulate or are prone to vertical translocation. We assumed that a high pH value at slightly calcareous soil properties should retain tetracyclines and fluoroquinolones even at high manure loads, whereas it should facilitate leaching of sulfonamides. Hence, we investigated the transport of manure-added antibiotics in a soil developed from calcareous parent material on a farm in peri-urban Beijing, China. Leachate was collected from zero-tension samplers, installed at 40cm depth under undisturbed soil, which was treated according to (i) routine farm practice and (ii) worst-case conditions (high antibiotic concentrations, irrigation imitating heavy rainfall). Additionally, the soil depth distribution of pharmaceuticals at the end of the leaching experiment was analysed. Under routine farm practice, sulfamethazine was repeatedly detected in the leachate with a maximum concentration of 0.12µgL(-1). All applied substances were still detected in soil after 53days, suggesting that there was no overall rapid and complete dissipation. Worst-case conditions enhanced vertical translocation; all leachate samples contained sulfonamides (up to 653µgL(-1) sulfamethazine), and even tetracyclines were found in the leachate once, with doxycycline reaching the highest concentration (0.19µgL(-1)). The concentrated simultaneous occurrence of sulfonamides with the bromide tracer in leachate samples pointed to preferential flow as underlying transport process. The high pH values did not prevent the single leaching event for tetracyclines and hardly affected overall leaching behaviour. The applied fluoroquinolones were not significantly translocated below 4cm depth, irrespective of irrigation and high manure addition.

Short term effects of copper, sulfadiazine and difloxacin on the anaerobic digestion of pig manure at low organic loading rates.

Antibiotics of inorganic and organic origin in pig manure can inhibit the anaerobic process in biogas plants. The influence of three frequently used antibiotics, copper dosed as CuSO(4), sulfadiazine (SDZ), and difloxacin (DIF), on the anaerobic digestion process of pig manure was studied in semi-continuous experiments. Biogas production recovered after every Cu dosage up to a sum of 12.94g Cukg(-1) organic dry matter (ODM), probably due to Cu precipitation following the formation of sulphide from sulphate. Complete inhibition was found at the very high Cu concentration of 19.40g Cukg(-1) ODM. Inhibitory effect of SDZ and DIF was observed at concentrations as high as 2.70gkg(-1) ODM and 0.54gkg(-1) ODM, respectively. It seems very unlikely that the antibiotics tested would inhibit the anaerobic process in a full-scale biogas plant.