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1.
J Miss State Med Assoc ; 56(3): 60-3, 2015 Mar.
Article in English | MEDLINE | ID: mdl-26050443

ABSTRACT

Increased survival of burn patients presents opportunities for reconstructive strategies to improve outcomes in management of acute and secondary burn injuries. To assess one such strategy, namely flap reconstruction, we reviewed cases performed during the first 4.5 years of the JMS Burn and Reconstruction Center. We found that flap procedures accounted for 0.8% of acute cases (23 of 2723 procedures) and 33% of secondary cases (260 of 790 procedures). This initial finding shows that in this practice flap procedures are applied to a small number of acute problems while flap procedures comprise 33% of secondary procedures. Reconstructive flap surgery plays a measurable role in burn treatment at this center. Further study of outcomes and timing could lead to better understanding of optimal strategies for flap reconstruction in burns.


Subject(s)
Burns/surgery , Surgical Flaps/statistics & numerical data , Aged, 80 and over , Burns/epidemiology , Humans , Incidence , Male , Middle Aged , Mississippi/epidemiology , Retrospective Studies
2.
J Plast Reconstr Aesthet Surg ; 63(11): 1849-52, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20106732

ABSTRACT

BACKGROUND: The Ten Test, first described in 1997 by Strauch et al., is a simple, rapid, reliable and sensitive method to evaluate hand sensibility in adults. In this study, we validated its use in children. METHODS: We asked patients to rate sensibility elicited by a light moving touch on the palmar surface of digits in reference to sensibility elicited by the same touch in a digit confirmed as normal. RESULTS: A total of 73 subjects (age range: 1-12 years) were tested. Patients under age 5 years were significantly less likely to complete the test. The kappa statistic for the Ten Test in nine subjects, each tested separately by two examiners, demonstrated very strong inter-observer reliability (kappa=1.0, p<0.003). CONCLUSIONS: The Ten Test is a simple, validated, non-threatening method to evaluate hand sensibility in children and adolescents. We recommend its clinical use in patients age 5 years and older.


Subject(s)
Hand/innervation , Neurologic Examination/methods , Sensation Disorders/diagnosis , Touch/physiology , Child , Child, Preschool , Diagnosis, Differential , Humans , Infant , Observer Variation , Reproducibility of Results , Retrospective Studies , Sensation Disorders/physiopathology
3.
J Reconstr Microsurg ; 25(3): 153-6, 2009 Mar.
Article in English | MEDLINE | ID: mdl-18925544

ABSTRACT

The thoracodorsal system represents a versatile, yet reliable system for the harvest of tissues for microsurgical transplantation. Also, the donor vessel anatomy is generally thought to be highly predictable in terms of topography and branching patterns. In this article we describe for the first time the occurrence of a branching pattern during a clinical harvest of the latissimus dorsi muscle flap, previously encountered only in cadaver dissections. We compare our intraoperative findings with published anatomical investigations and discuss the implications of this particular variation of the blood supply to the latissimus dorsi muscle for clinical practice.


Subject(s)
Foot Injuries/surgery , Muscle, Skeletal/transplantation , Surgical Flaps/blood supply , Thorax/blood supply , Axilla/surgery , Humans , Male , Microsurgery/methods , Middle Aged , Muscle, Skeletal/blood supply , Thoracic Surgical Procedures/methods
5.
Ann Plast Surg ; 60(5): 589-93, 2008 May.
Article in English | MEDLINE | ID: mdl-18434837

ABSTRACT

The effect of vascular endothelial growth factor (VEGF) on skin graft survival was studied in rats. Models of skin grafting on muscle and periosteum were designed. For the study of skin grafting on muscle model, 32 rats were divided into 4 groups. VEGF was administrated systemically after skin graft placement intrafascially injected into the recipient bed at the time of graft placement and topically applied to the recipient bed at the time of graft placement. Control groups consisted of grafts placed on sites without systemic or local VEGF treatment. With the study of skin grafting on periosteum, 40 rats were divided into 4 groups. VEGF was systemically, intraperiosteally, and topically applied to the recipient bed. The control animals received no treatment. On the fifth postoperative day, the survival area of each skin graft was measured and the graft was harvested for histology with CD31 immunohistochemical staining. The results showed that in skin grafting on muscle model, the mean viable percentage of the skin graft was 66.1% +/- 10.2% in the group receiving systemic application of VEGF and 56.1% +/- 9.8% in the group receiving VEGF intrafascia injection. The survival percentages were significantly higher than those found in the control group (22.5% +/- 7.7%) and the group with VEGF topical application (30.8% +/- 4.1%). In skin grafting on periosteum, the group receiving VEGF intraperiosteum injection reached a survival percentage of 50.5% +/- 4.3%, significantly higher than the groups with VEGF systemic application (28.7 +/- 5.5%), VEGF topical application (32.5% +/- 4.8%), and the control (25.8% +/- 6.0%). Histology showed that sections taken from grafts with VEGF intrafascia and intraperiosteum treatment revealed angiogenesis. The data demonstrated that administration of VEGF into the either muscular or periosteal recipient beds for skin grafting can improve the skin graft survival.


Subject(s)
Angiogenesis Inducing Agents/administration & dosage , Graft Survival/drug effects , Skin Transplantation , Vascular Endothelial Growth Factor A/administration & dosage , Animals , Disease Models, Animal , Male , Necrosis/prevention & control , Rats , Rats, Sprague-Dawley
6.
Ann Plast Surg ; 58(1): 61-3, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17197944

ABSTRACT

In microsurgical flap procedures, creation of an arteriovenous fistula (AVF) is a technique of vein grafting where the vein graft is connected to recipient vessels as a flow-through loop prior to harvest and inset of the flap. Controversy exists whether this technique can be used as a 2-stage procedure with the loop and flap transfer accomplished in sequential operations or if the loop and flap transfer should be performed in a single operation. We performed 12 consecutive 1-stage AVF-flap procedures, with 1 flap failure. We combined this series with previously published reports to compare outcomes of 1-stage and 2-stage procedures. We found no significant difference in flap outcomes or complication rates between the 2 strategies. We conclude from our experience and this analysis that single-stage AVF-flap procedures are the optimum application of this technique.


Subject(s)
Arteriovenous Anastomosis , Plastic Surgery Procedures/methods , Surgical Flaps/blood supply , Adolescent , Adult , Female , Humans , Male , Microsurgery , Middle Aged , Time Factors
7.
J Reconstr Microsurg ; 22(1): 5-14, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16425115

ABSTRACT

Reconstruction of large defects of the pelvis and groin sometimes requires microsurgical flaps when the otherwise rich supply of local options fails to be sufficient in difficult and complicated cases. Recipient vessels for microsurgical flaps to the groin and pelvis are important elements in planning such procedures. Seven groups have reported pelvis and groin microsurgical flap cases, and their recipient vessels have included the superior and inferior gluteal vessels, the femoral vessels, the deep perforators of the femoral system, the inferior epigastric vessels, intra-abdominal vessels, and interpositional vein grafts to distant recipient vessels. This review summarizes their experience and describes the recipient vessel options in the pelvic and groin region.


Subject(s)
Arteries/surgery , Groin/blood supply , Pelvis/blood supply , Surgical Flaps/blood supply , Veins/surgery , Graft Survival , Groin/surgery , Humans , Microsurgery/methods , Pelvis/surgery
8.
J Reconstr Microsurg ; 20(6): 483-8, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15356771

ABSTRACT

The authors examined the preservation of rat gracilis muscle flap mass after motor and sensory end-to-side neurorrhaphy. The rat gracilis muscle flap model was designed based on a previous study. Twenty-four Sprague-Dawley rats were divided into three groups. In Group 1 (n = 8), the flap was denervated by transecting the obturator nerve. In Group 2 (n = 8), the flap was reinnervated by coapting the proximal saphenous nerve to the distal obturator nerve. In Group 3 (n = 8), the flap was reinnervated by coapting the motor branch of the femoral nerve to the distal stump of the obturator nerve. At 6 months postoperatively, the gracilis muscle flaps were examined, harvested, and weighed individually. Results showed that the flaps with motor nerve reinnervation retained good bulk, with a weight of 634.0 +/- 65.1 gm, which was statistically significantly higher than the denervated group (457.5 +/- 125.3 gm, p < 0.01). However, muscle mass preservation in the sensory reinnervated group (606.9 +/- 209.1 gm) was not significantly different, compared to the denervated group. Histology revealed atrophic changes in the denervated group, compared to the sensory and motor-reinnervated groups. The authors concluded that muscle mass can be preserved by end-to-side nerve repair. Motor nerve reinnervation is able to better arrest atrophic changes of the muscle flaps.


Subject(s)
Surgical Flaps , Animals , Femoral Nerve/surgery , Muscle Denervation , Muscle, Skeletal/innervation , Muscle, Skeletal/pathology , Muscle, Skeletal/surgery , Nerve Transfer , Obturator Nerve/surgery , Rats , Rats, Sprague-Dawley , Surgical Flaps/blood supply , Surgical Flaps/innervation
9.
Ann Surg ; 239(6): 866-73; discussion 873-5, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15166966

ABSTRACT

OBJECTIVE: Cytokines may be a mechanism by which surgical delay can increase flap survival. We previously found that preoperative vascular endothelium growth factor (VEGF) administration in the rat transverse rectus abdominis myocutaneous (TRAM) flap could improve skin paddle survival. In this study, we used partial elevation of the rat TRAM flap as a surgical delay to assess endogenous cytokine expression and tissue survival comparable to undelayed TRAM flaps. METHODS: In Part I, TRAM flaps underwent surgical delay procedures; 7 days later, the flaps were completely elevated and reinset. At the same time, other flaps were raised and reinset without delay. Skin paddle survival in both groups was evaluated at 7 days. In Part II, skin biopsies from TRAM zones I to IV were taken at the time of delay and at intervals of 12, 24, 48, and 72 hours. Specimens were assessed for selected cytokine gene expression by reverse transcription-polymerase chain reaction analysis (TR-PCR). RESULTS: Surgical delay significantly (P < 0.001) increased skin paddle survival in the delayed TRAM flaps (16.14 +/- 1.53 cm, 81.9%) compared with undelayed flaps (7.68 +/- 3.16 cm, 40.9%). TGF-beta and PDGF expressions were not changed by surgical delay, but basic fibroblast growth factor (bFGF) and VEGF expressions increased significantly (P < 0.05 and P < 0.01) after delay. CONCLUSIONS: In the rat TRAM model, surgical delay resulted in increased VEGF expression and increased skin paddle survival. These results correlate with previous studies showing the preoperative injection of VEGF increases skin paddle survival. VEGF may be an important element in the delay phenomenon and may be an agent for pharmacological delay.


Subject(s)
Rectus Abdominis/transplantation , Surgical Flaps/pathology , Vascular Endothelial Growth Factors/pharmacology , Analysis of Variance , Animals , Biopsy, Needle , Disease Models, Animal , Graft Survival/drug effects , Immunohistochemistry , Male , Probability , RNA, Messenger/analysis , Rats , Rats, Sprague-Dawley , Reference Values , Reverse Transcriptase Polymerase Chain Reaction , Sensitivity and Specificity , Time Factors , Tissue Transplantation/adverse effects , Tissue Transplantation/methods
10.
J Reconstr Microsurg ; 19(7): 467-72, 2003 Oct.
Article in English | MEDLINE | ID: mdl-14634910

ABSTRACT

In this study, the authors examined the effects of fibronectin and laminin on sciatic nerve regeneration in rats. Sixty-eight Sprague-Dawley rats underwent bilateral sciatic nerve transections and silicone tubulizations, with a 10-mm gap between the proximal and distal nerve stumps. Thirty rats (n=30) received 10 microg of fibronectin injection into the right sciatic nerve chamber, while saline was injected into the left nerve chamber, serving as the control. Another 30 rats (n=30) were given 6 microg of laminin injection into the right nerve chambers and saline into the left chambers. At 1, 3, and 4 months postoperatively, electrophysiologic and histologic examinations, including nerve morphometry, were performed. Eight additional rats, receiving fibronectin (n=4) and laminin (n=4) injections, were used for horseradish peroxidase (HRP) tracing at 3 months postoperatively. Results from the study showed that fibronectin- and laminin-treated groups had significantly higher motor nerve conduction velocity and evoked muscle action potential amplitude of the anterior tibial muscle than the control group (p<0.01). Nerve diameter and the number of myelinated axons from the groups receiving fibronectin and laminin applications were greater than the controls (p<0.01). Also, a greater number of HRP-labeled motor neurons were found in the ventral horns and dorsal root ganglia of the fibronectin- and laminin-treatment groups compared to the controls. The authors conclude that local applications of fibronectin and laminin into the nerve chambers can significantly improve axonal regeneration and maturation of injured rat sciatic nerves.


Subject(s)
Fibronectins/pharmacology , Laminin/pharmacology , Nerve Regeneration/drug effects , Sciatic Nerve/physiology , Action Potentials/drug effects , Animals , Axis, Cervical Vertebra , Horseradish Peroxidase , Models, Animal , Rats , Rats, Sprague-Dawley
11.
Plast Reconstr Surg ; 112(6): 1613-9, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14578792

ABSTRACT

This study evaluated the effect of exogenous vascular endothelial growth factor (VEGF) on tendon healing and regulation of other growth factors in a rat Achilles tendon model. Fifty Sprague-Dawley rats were used. In the experimental group, the left Achilles tendon was transected and repaired with the modified Kessler suture technique, and the right Achilles tendon was transected and repaired with resection of plantaris tendon. VEGF, 100 mul (50 mug/ml), was injected into each tendon at the repair site. The same surgical procedures were performed in the control group, with the same volume of saline injected into the repair sites. At intervals of 1, 2, and 4 weeks, the animals were killed and the tendons were harvested and evaluated for tensile strength (1, 2, and 4 weeks) and gene expression (postoperative day 4). At 1 week postoperatively, when plantaris tendon was preserved, the tensile strength of the repaired tendons with VEGF treatment (3.63 +/- 0.62 MPa) was significantly higher than the tensile strength of the repaired tendons with saline treatment (2.20 +/- 0.36 MPa). There was no difference in tensile strength between the two groups without the plantaris tendon support. At 2 weeks postoperatively, the tensile strength was 11.34 +/- 3.89 MPa in the group with VEGF treatment and plantaris tendon preservation, which was significantly higher than the tensile strength in the other groups. There was no significant difference in tensile strength among the groups at 4 weeks postoperatively. The gene expression showed that transforming growth factor-beta in the VEGF-treated tendon was up-regulated in the early stage of tendon healing, whereas expression of platelet-derived growth factor, basic fibroblast growth factor, and insulin-like growth factor-1 was not significantly different among the groups. In conclusion, administration of exogenous VEGF can significantly improve tensile strength early in the course of the rat Achilles tendon healing and was associated with increased expression of transforming growth factor-beta.


Subject(s)
Achilles Tendon/injuries , Vascular Endothelial Growth Factor A/pharmacology , Wound Healing/drug effects , Achilles Tendon/metabolism , Achilles Tendon/physiopathology , Achilles Tendon/surgery , Animals , Fibroblast Growth Factor 2/genetics , Fibroblast Growth Factor 2/metabolism , Gene Expression , In Vitro Techniques , Insulin-Like Growth Factor I/genetics , Insulin-Like Growth Factor I/metabolism , Male , Platelet-Derived Growth Factor/genetics , Platelet-Derived Growth Factor/metabolism , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Tensile Strength , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/metabolism
12.
Plast Reconstr Surg ; 112(6): 1620-7, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14578793

ABSTRACT

This study evaluated the effect of phenytoin (sodium diphenylhydantoin) on skin wound healing in a rat model. The study was divided into two parts. In part I, 20 mul of phenytoin (10 mg/ml) was subcutaneously injected into the 3-cm dorsal full-thickness incisional wounds of 14 rats on postoperative days 0, 3, and 6. Twelve rats that received saline injections were used as the controls. The skin samples were harvested and tested for tensile strength and histology. An additional 12 rats with the same incisional wounds were tested for chemokine gene expressions. In part II, 20 mul of phenytoin (10 mg/ml) was applied topically once a day on a 4 x 4 cm area of the open dorsal wounds of 10 rats. Saline was applied to the wounds of the 10 control group rats. The wounds were measured weekly. The results showed that the average tensile strength of the phenytoin-treated wound was 0.49 +/- 0.08 MPa compared with the control group at 0.02 +/- 0.01 MPa (p < 0.05). The density ratio of chemokine monocyte chemotactic protein (MCP-1) to beta-actin in the phenytoin-treated group was also significantly higher than in the control group (p < 0.05). Histologic analysis of the phenytoin group showed a large amount of fibroblast proliferation, collagen synthesis, and neovascularization. Phenytoin-treated wounds were also smaller at 1 to 6 weeks postoperatively than the control group wounds. The authors conclude that the administration of phenytoin can promote wound healing and significantly increase MCP-1 expression. Phenytoin-treated wounds showed significant increase in collagen deposition and neovascularization, which resulted in an increased wound tensile strength and accelerated healing of both open and closed wounds.


Subject(s)
Phenytoin/pharmacology , Skin/injuries , Wound Healing/drug effects , Actins/genetics , Actins/metabolism , Animals , Chemokine CCL2/genetics , Chemokine CCL2/metabolism , Chemokines/metabolism , Fibroblasts/pathology , Gene Expression , In Vitro Techniques , Neovascularization, Pathologic , Neutrophils/pathology , Rats , Rats, Sprague-Dawley , Skin/metabolism , Skin/pathology , Skin/physiopathology , Tensile Strength , Wound Healing/physiology
13.
South Med J ; 96(7): 689-91, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12940322

ABSTRACT

In obese patients, lymphodystrophic tissue can create large masses that are microscopically indistinguishable from chronic lymphedema. This tissue can be disabling, especially in the lower extremities. The tissue is refractory to conservative therapy and is prone to cellulitis and abscess formation. The patients are regarded as being at high risk for surgical complications due to obesity and related illnesses. We report two cases of patients with lesions severely limiting walking. The two patients weighed 490 and 520 lb, respectively. One patient had hypertension and asthma; the other had arrhythmias and chronic venous thrombosis. One patient had wedge resection of the right groin (23 lb) and knee (5 lb), and the second patient had resection of the right thigh (65 lb) and left thigh (84 lb). All procedures were done separately. Both patients reported improvement in walking. Simple wedge excision was an effective, minimally complicated treatment for these patients.


Subject(s)
Angiolipoma/surgery , Leg/surgery , Lymphedema/surgery , Obesity, Morbid/complications , Skin Neoplasms/surgery , Soft Tissue Neoplasms/surgery , Adult , Angiolipoma/pathology , Arrhythmias, Cardiac/complications , Connective Tissue/pathology , Female , Humans , Leg/pathology , Lymphedema/pathology , Male , Obesity, Morbid/pathology , Obesity, Morbid/surgery , Risk , Skin/pathology , Skin Neoplasms/pathology , Soft Tissue Neoplasms/pathology , Venous Thrombosis/complications , Walking
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