ABSTRACT
The aim of this study was to assess patterns of CCND3 gene amplification in bladder cancer and correlate gene status with recurrence-free and progression-free survival. A sequential cohort series of 102 primary bladder tumor samples in which there was enough tissue material to assess CCND3 gene status by fluorescent in situ hybridization (FISH) was the study group. CCND3 gene FISH amplification present in 31.4 percent of bladder carcinomas, was related to tumor progression (p=0.021) and lower time to progression (mean+-SD; 25.75+-15.25 months) as compared to 33.29+-11.0 months in the CCND3 not amplified group (p=0.05). By immunohistochemistry, Cyclin D3 labeling index was higher in the CCND3 amplified group (mean+-SD, 76.69+-27.51) than in not amplified (mean+-SD, 21.57+-7.02) (p less than 0.0001). The univariate survival analysis showed CCND3 gene amplification to be associated to a shorter progression-free survival (p=0.020) together with WHO histological grade (p=0.001) and pT stage category (p less than 0.0001). Coxs regression analysis selected CCND3 amplification as an independent predictor of progression-free survival (p= 0.030, RR3.561, 95 percent CI 1.128-11.236) together with pT category (p less than 0.0001, RR5.834, 95 percent CI 2.364-14.395). Our FISH analysis suggests that CCND3 gene amplification is a marker of aggressiveness and might be a predictor of tumor progression in bladder urothelial carcinoma.
Subject(s)
Biomarkers, Tumor/genetics , Cyclin D3/genetics , In Situ Hybridization, Fluorescence/methods , Urinary Bladder Neoplasms/genetics , Aged , Disease Progression , Female , Humans , Male , Middle Aged , Urinary Bladder Neoplasms/mortalityABSTRACT
CONTEXT: C-reactive protein (CRP), predicts coronary heart disease incidence in healthy subjects and has been associated with decreased endothelium-dependent relaxation, a potential risk factor for hypertension. However, the relationship between CRP and hypertension has not been studied. OBJECTIVE: To assess whether circulating levels of CRP are independently related to essential hypertension. DESIGN: Cross-sectional population survey. We measured circulating levels of CRP, blood pressure and cardiovascular risk factors among participants. Binomial regression was used to calculate the adjusted effect of CRP on the prevalence of hypertension. SETTING: General community of Bucaramanga, Colombia. PARTICIPANTS: A random sample of 300 subjects > or = 30 years old. MAIN OUTCOME MEASURE: Arterial blood pressure. RESULTS: Overall hypertension prevalence was 46.0%. The unadjusted prevalence of hypertension was 58.7% in the highest quartile of CRP, but only 34.7% in the lowest quartile. After adjustment for age, sex, body mass index, family history of hypertension, fasting glycemia, sedentary behaviour, and alcohol consumption, the prevalence of hypertension was 1.14 [95% confidence interval (CI), 0.82, 1.58; P= 0.442], 1.36 (95% CI, 0.99, 1.87; P= 0.057) and 1.56 (95% CI, 1.14, 2.13; P = 0.005) times higher in subjects in the second, third and fourth quartiles of CRP, as compared to subjects in the first quartile. CONCLUSIONS: Our results suggest, for the first time, that CRP level may be an independent risk factor for the development of hypertension. However, because of the cross-sectional nature of our study, this finding should be confirmed in prospective cohort studies, aimed at elucidating the role of CRP in the prediction, diagnosis and management of hypertension.
Subject(s)
C-Reactive Protein/analysis , Hypertension/etiology , Aged , Cross-Sectional Studies , Female , Humans , Hypertension/epidemiology , Italy , Male , Middle Aged , Prevalence , Risk FactorsABSTRACT
Se presenta un caso de meningocele intratoracico tratado quirurgicamente por el metodo de obturacion del antro medular por imbricacion de colgajos de las paredes. Se comenta la extrema infrecuencia de esta localizacion en la bibliografia universal.En la nacional, este seria el primer caso observado. Se advierte sobre la concomitancia con afecciones vertebrales por defecto oseo congenito y con la neurofibromatosis de Recklinghausen. Se establece que la reseccion quirurgica puede ser fuente de complicaciones neurologicas, tales como la infeccion y la hidrocefalia
Subject(s)
Middle Aged , Humans , Male , MeningoceleABSTRACT
Se presenta un caso de meningocele intratoracico tratado quirurgicamente por el metodo de obturacion del antro medular por imbricacion de colgajos de las paredes. Se comenta la extrema infrecuencia de esta localizacion en la bibliografia universal.En la nacional, este seria el primer caso observado. Se advierte sobre la concomitancia con afecciones vertebrales por defecto oseo congenito y con la neurofibromatosis de Recklinghausen. Se establece que la reseccion quirurgica puede ser fuente de complicaciones neurologicas, tales como la infeccion y la hidrocefalia
