Adaptive and Robust Operation with Active Fuzzy Harvester under Nonstationary and Random Disturbance Conditions.

The objective of this paper is to amplify the output voltage magnitude from a piezoelectric vibration energy harvester under nonstationary and broadband vibration conditions. Improving the transferred energy, which is converted from mechanical energy to electrical energy through a piezoelectric transducer, achieved a high output voltage and effective harvesting. A threshold-based switching strategy is used to improve the total transferred energy with consideration of the signs and amplitudes of the electromechanical conditions of the harvester. A time-invariant threshold cannot accomplish effective harvesting under nonstationary vibration conditions because the assessment criterion for desirable control changes in accordance with the disturbance scale. To solve this problem, we developed a switching strategy for the active harvester, namely, adaptive switching considering vibration suppression-threshold strategy. The strategy adopts a tuning algorithm for the time-varying threshold and implements appropriate intermittent switching without pre-tuning by means of the fuzzy control theory. We evaluated the proposed strategy under three realistic vibration conditions: a frequency sweep, a change in the number of dominant frequencies, and wideband frequency vibration. Experimental comparisons were conducted with existing strategies, which consider only the signs of the harvester electromechanical conditions. The results confirm that the presented strategy achieves a greater output voltage than the existing strategies under all nonstationary vibration conditions. The average amplification rate of output voltage for the proposed strategy is 203% compared with the output voltage by noncontrolled harvesting.

Nitrate salts suppress sporulation and production of enterotoxin in Clostridium perfringens strain NCTC8239.

Clostridium perfringens type A is a common source of food-borne illness in humans. Ingested vegetative cells sporulate in the small intestinal tract and in the process produce C. perfringens enterotoxin (CPE). Although sporulation plays a critical role in the pathogenesis of food-borne illness, the molecules triggering/inhibiting sporulation are still largely unknown. It has previously been reported by our group that sporulation is induced in C. perfringens strain NCTC8239 co-cultured with Caco-2 cells in Dulbecco's Modified Eagle Medium (DMEM). In contrast, an equivalent amount of spores was not observed when bacteria were co-cultured in Roswell Park Memorial Institute-1640 medium (RPMI). In the present study it was found that, when these two media are mixed, RPMI inhibits sporulation and CPE production induced in DMEM. When a component of RPMI was added to DMEM, it was found that calcium nitrate (Ca[NO3 ]2 ) significantly inhibits sporulation and CPE production. The number of spores increased when Ca(NO3 )2 -deficient RPMI was used. The other nitrate salts significantly suppressed sporulation, whereas the calcium salts used did not. qPCR revealed that nitrate salts increased expression of bacterial nitrate/nitrite reductase. Furthermore, it was found that nitrite and nitric oxide suppress sporulation. In the sporulation stages, Ca(NO3 )2 down-regulated the genes controlled by Spo0A, a master regulator of sporulation, but not spo0A itself. Collectively, these results indicate that nitrate salts suppress sporulation and CPE production by down-regulating Spo0A-regulated genes in C. perfringens strain NCTC8239. Nitrate reduction may be associated with inhibition of sporulation.

Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.

In order to clone and analyse the avirulence gene AVR-Pia from Japanese field isolates of Magnaporthe oryzae, a mutant of the M. oryzae strain Ina168 was isolated. This mutant, which was named Ina168m95-1, gained virulence towards the rice cultivar Aichi-asahi, which contains the resistance gene Pia. A DNA fragment (named PM01) that was deleted in the mutant and that co-segregated with avirulence towards Aichi-asahi was isolated. Three cosmid clones that included the regions that flanked PM01 were isolated from a genomic DNA library. One of these clones (46F3) complemented the mutant phenotype, which indicated clearly that this clone contained the avirulence gene AVR-Pia. Clone 46F3 contained insertions of transposable elements. The 46F3 insert was divided into fragments I-VI, and these were cloned individually into a hygromycin-resistant vector for the transformation of the mutant Ina168m95-1. An inoculation assay of the transformants revealed that fragment V (3.5 kb) contained AVR-Pia. By deletion analysis of fragment V, AVR-Pia was localized to an 1199-bp DNA fragment, which included a 255-bp open reading frame with weak homology to a bacterial cytochrome-c-like protein. Restriction fragment length polymorphism analysis of this region revealed that this DNA sequence co-segregated with the AVR-Pia locus in a genetic map that was constructed using Chinese isolates.