ABSTRACT
Here we present a protocol to engineer apical-out airway organoids (AOAOs) directly from human airway basal stem cells (hABSCs) using suspension culture of hABSC aggregates on a cell-repellent surface. We describe steps to produce spherical AOAOs with homogenous presentation of exterior-facing motile cilia and of tunable sizes. We then detail procedures to analyze AOAO cellular composition via wholemount staining and assess cilia motility via 3D AOAO rotation upon Matrigel embedding. The protocol offers an effective model for investigating human airway pathophysiology. For complete details on the use and execution of this protocol, please refer to Wijesekara et al. (2022).1.
ABSTRACT
BACKGROUND: According to the U.S. Census, 63.7% of the population is Caucasian, whereas 36.3% are minorities. In the United States, 33.1% of RNs are considered ethnic minorities whereas in Nebraska only 6.3% of RNs are minorities. Specific measures are needed to prepare a more diverse nursing workforce. The use of specific supportive methods directed toward educationally disadvantaged and minority nursing students may improve retention. METHOD: The University of Nebraska Medical Center College of Nursing-implemented the Health Resources and Services Administration funded Generation Link to Learn (LTL) to help educationally disadvantaged students to be successful in a baccalaureate nursing degree (BSN) program of study. RESULTS: Students in the LTL program were successful in the BSN program, with 88% (24 of 27) of students graduating. CONCLUSION: Multiple supports, including robust academic coaching and scholarships, are effective strategies to help educationally disadvantaged nursing students. [J Nurs Educ. 2019;58(11):661-664.].
