ABSTRACT
Abstract Mass production of predatory stinkbugs in the laboratory is prioritized to release them into the field as part of IPM programs. Therefore, the aim of this study was to identify the best prey for rearing the predator Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae) among five different species of insect (three of Lepidoptera, one of Coleoptera, and one of Diptera). Second-instar P. nigrispinus nymphs were conditioned in transparent 1000-mL plastic pots, adults were placed in Petri dishes for mating, and both stages were maintained under controlled conditions (25 ± 1°C, 12 hours of photophase, 70 ± 10% RH). Nymphs and adults of P. nigrispinus consumed more Musca domestica (Linnaeus, 1758) (Diptera: Muscidae) larvae than the other tested prey. The consumption of fly larvae was 1.5 larvae/day/nymph and adults 1.7 larvae/day/adult. However, the number of eggs per female was less when the predator consumed M. domestica larvae (407.8 eggs/female) and most when consumed the Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) larvae (797.7 eggs/female). Furthermore, the percentage of hatched eggs was greater when the predator females consumed D. saccharalis larvae (90.0%). D. saccharalis larvae is the best prey to rearing P. nigrispinus.
Resumo A produção massal de percevejos predadores no laboratório é priorizada para liberá-los no campo, como parte de programas de MIP. Portanto, o objetivo deste estudo foi identificar a melhor presa para a criação do predador Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae) entre cinco espécies diferentes de insetos (três de Lepidoptera, uma de Coleoptera e uma de Diptera). Ninfas de segundo ínstar de P. nigrispinus foram acondicionadas em recipientes plásticos transparentes de 1000 ml, adultos foram colocados em placas de Petri para o acasalamento e, ambos os estágios foram mantidos sob condições controladas (25 ± 1°C, fotofase de 12 horas e 70 ± 10% UR). Ninfas e adultos de P. nigrispinus consumiram mais larvas de Musca domestica (Linnaeus, 1758) (Diptera: Muscidae) do que as outras presas testadas. O consumo de larvas de mosca foi de 1,5 larvas/dia/ninfa e adultos 1,7 larvas/dia/adulto. No entanto, o número de ovos por fêmea foi menor quando o predador consumiu larvas de M. domestica (407,8 ovos/fêmea) e maior quando consumiu larvas de Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) (797,7 ovos/fêmea). Além disso, a percentagem de eclosão de ninfas foi maior quando as fêmeas do predador consumiram larvas de D. saccharalis larvas (90,0%). Larvas de D. saccharalis é a melhor presa para a criação de P. nigrispinus.
Subject(s)
Animals , Female , Sexual Behavior, Animal/physiology , Coleoptera/physiology , Food Chain , Hemiptera/physiology , Lepidoptera/physiology , Reproduction/physiology , Diptera/physiology , Life Cycle Stages/physiologyABSTRACT
Mass production of predatory stinkbugs in the laboratory is prioritized to release them into the field as part of IPM programs. Therefore, the aim of this study was to identify the best prey for rearing the predator Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae) among five different species of insect (three of Lepidoptera, one of Coleoptera, and one of Diptera). Second-instar P. nigrispinus nymphs were conditioned in transparent 1000-mL plastic pots, adults were placed in Petri dishes for mating, and both stages were maintained under controlled conditions (25 ± 1°C, 12 hours of photophase, 70 ± 10% RH). Nymphs and adults of P. nigrispinus consumed more Musca domestica (Linnaeus, 1758) (Diptera: Muscidae) larvae than the other tested prey. The consumption of fly larvae was 1.5 larvae/day/nymph and adults 1.7 larvae/day/adult. However, the number of eggs per female was less when the predator consumed M. domestica larvae (407.8 eggs/female) and most when consumed the Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) larvae (797.7 eggs/female). Furthermore, the percentage of hatched eggs was greater when the predator females consumed D. saccharalis larvae (90.0%). D. saccharalis larvae is the best prey to rearing P. nigrispinus.
Subject(s)
Coleoptera/physiology , Food Chain , Hemiptera/physiology , Lepidoptera/physiology , Sexual Behavior, Animal/physiology , Animals , Diptera/physiology , Female , Life Cycle Stages/physiology , Reproduction/physiologyABSTRACT
We evaluated the effects of constant low-temperature storage on Diaeretiella rapae (McIntosh) (Braconidae, Aphidiinae). Diaeretiella rapae mummies were stored at 5 ± 1°C for 0-36 days. The percentage of D. rapae emergence varied (100-83%) after 0-32 days of storage. After 32 days, emergence reduced to 55%. According to our Probit analysis, 50% mortality (LT50) of the population of D. rapae was reached after 40 days of storage at 5°C. Storage for up to 32 days did not negatively affect emergence and survival. Cold exposure of D. rapae for 36 days did not influence morphological malformations, sex ratio, and emergence of the F1 generation. After 4-36 days of storage, D. rapae showed a gradual decrease in emergence, longevity, reproductive capacity, and F1 sex ratio. Diaeretiella rapae can be stored for up to 24 days at 5°C, at which time the percentage of parasitism and the F1 sex ratio remain above 38% and at 0.50, respectively.
