ABSTRACT
AIMS: To identify lactic acid bacteria (LAB) present in Moroccan dairy products to establish and preserve their microbial species diversity. METHODS AND RESULTS: Thirty-seven samples were collected from different farms. A total of 146 LAB were isolated and subjected to (GTG)(5)-PCR analysis. Comparison of the profiles with data available at the Moroccan Coordinated Collections of Micro-organisms allowed identification of 85 isolates. The remaining 61 were subjected to SDS-PAGE analysis of whole cell proteins. Comparison of the profiles with data available at the Belgian Coordinated Collections of Micro-organisms allowed identification of 43 isolates. Several of the remaining 18 isolates exhibited identical protein electrophoretic fingerprints. Therefore, eight representatives of them were subjected to partial pheS gene sequencing which allowed identification of all remaining isolates. In raw milk, six genera were found while in 'lben', three were found. This is the first report of Leuconostoc kimchii in dairy products. CONCLUSIONS: LAB diversity was established using a stepwise polyphasic identification approach. It used the expertise of both research bodies involved in this study and proved to be cost-effective for the identification of all isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: To establish LAB diversity in Moroccan dairy products which could be a source of strains with specific properties.
Subject(s)
Food Microbiology , Lactobacillaceae/isolation & purification , Milk/microbiology , Animals , Bacterial Typing Techniques , Cultured Milk Products/microbiology , DNA Fingerprinting , DNA, Bacterial/genetics , Electrophoresis, Polyacrylamide Gel , Lactobacillaceae/classification , Lactobacillaceae/genetics , Leuconostoc/classification , Leuconostoc/genetics , Leuconostoc/isolation & purification , MoroccoABSTRACT
PURPOSE OF THE STUDY: We present our experience with a rare localization of tuberculosis, the wrist, focusing on symptoms and outcome after treatment. MATERIAL AND METHODS: Our series included eleven patients, eight men and three women, mean age 42 Years, who presented tuberculosis of the wrist. Tuberculosis was known in four patients who were being treated and a context of tuberculosis was recognized in three others. One patient on long-term corticosteroid therapy was immunodepressed. Mean time from symptom onset to consultation was fifteen months indicating a slow and progressive disease process. An inflammatory syndrome was noted in nine patients. Based on the standard x-rays, the David-Chausse classification was: stage I n=1; stage II n=1; stage III n=3; stage IV n=4. The AP view of the wrist was normal in two patients. Diagnosis of tuberculosis was confirmed on the surgical biopsy specimen which revealed epithelio-gigantocellulary granuloma with caseous necrosis. In only five patients Koch bacilli developed in culture on Lowenstein-Jensen. Patients were given anti-tuberculous antibiotics and the wrist was immobilized in a plaster splint. RESULTS: Mean follow-up was two years. The disease course was blunted by the antituberculosis treatment. Surgical drainage was only required to clear abscesses. Wrist stiffness was frequent and had a significant functional impact. DISCUSSION: These eleven cases of a rare localization of tuberculosis illustrate the slow progressive course of clinical symptoms and radiological signs of tuberculosis, emphasizing the difficulties encountered in establishing early diagnosis of such isolated non-abscessed localizations. Anti-tuberculous treatment is effective but the functional outcome depends on early diagnosis before the development of radiological evidence of joint destruction.
Subject(s)
Carpal Bones , Tuberculoma/diagnosis , Tuberculoma/therapy , Tuberculosis, Osteoarticular/diagnosis , Tuberculosis, Osteoarticular/therapy , Wrist Joint , Abscess/classification , Abscess/diagnosis , Abscess/etiology , Abscess/therapy , Adult , Antitubercular Agents/therapeutic use , Biopsy , Combined Modality Therapy , Diagnosis, Differential , Disease Progression , Drainage , Female , Follow-Up Studies , Hand Strength , Humans , Magnetic Resonance Imaging , Male , Range of Motion, Articular , Severity of Illness Index , Splints , Time Factors , Tomography, X-Ray Computed , Treatment Outcome , Tuberculoma/classification , Tuberculoma/etiology , Tuberculosis, Osteoarticular/classification , Tuberculosis, Osteoarticular/etiologyABSTRACT
We observed a pathological fracture of the femur in a 21-year-old woman with a venous malformation. Physical examination and plain x-rays disclosed a pathological bone. Biopsy revealed the venous malformation. The extent of the lesion that involved the entire quadriceps muscle was detailed with duplex-Doppler, computed tomography and arteriography. The patient was treated with an intramedullary nail. Intra-operative bleeding necessitated transfusion (packed cells and frozen plasma). Outcome was favorable. Bone healing was achieved at seven months.
Subject(s)
Calcinosis/complications , Femoral Fractures/etiology , Fractures, Spontaneous/etiology , Leg/blood supply , Vascular Diseases/complications , Veins/abnormalities , Adult , Female , HumansABSTRACT
One case of divergent dislocation of the elbow is reported. It was associated with other lesions of the upper limb due to the violence of the initial trauma. The authors found 6 other cases reported in the literature. They discuss the mechanism, diagnosis and treatment of such lesions.