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Diagn Microbiol Infect Dis ; 101(4): 115494, 2021 Dec.
Article in English | MEDLINE | ID: mdl-34391980

ABSTRACT

Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan. The developed assay could detect as low as 1 genome copy of M. avium DNA within 30 minutes. All 91 (100%) M. avium isolates from pigs were detected positive while all other tested bacterial species were negative. Interestingly, among the 137 clinical M. avium isolates, 41 (30%) were undetectable with this LAMP assay as they lacked IS1245, the absence of which was revealed by PCR and whole-genome sequencing. These findings highlighted genotypic differences in M. avium strains from humans and pigs in Japan and how this diversity can influence the applicability of a detection tool across different geographic areas and hosts.


Subject(s)
DNA Transposable Elements/genetics , Molecular Diagnostic Techniques/methods , Mycobacterium avium/genetics , Nucleic Acid Amplification Techniques/methods , Animals , Genetic Variation , Genome, Bacterial/genetics , Humans , Japan , Mycobacterium Infections/microbiology , Mycobacterium Infections/veterinary , Mycobacterium avium/classification , Mycobacterium avium/isolation & purification , Polymerase Chain Reaction , Sensitivity and Specificity , Species Specificity , Swine , Swine Diseases/microbiology
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