Prevalence and risk factors of musculoskeletal disorders among Tunisian nurses.

BACKGROUND: Musculoskeletal problems depict essentially the most worrying difficulties inside work health today. AIMS: To evaluate the prevalence of musculoskeltal issues and to search relationships with individual characteristics and work-related risk factors among two public hospitals nurses in Sousse. METHODS: It is a descriptive-correlational investigation based on a self-administered questionnaire destined for all nurses working in two public hospitals of Sousse. The potential risk of musculoskeletal issues was examined by logistical regression. RESULTS: The prevalence of musculoskeletal disorders (MSD) over the last twelve months was 48.1%. Musculoskeletal problems developed mainly in low back (68.5%), upper back (36.9%), and knees (34.5%). The Chi-square test indicated that MSD is associated with female gender (p = 0.01), being single (p = 0.013), high BMI (p <0.001), seniority (p =0.001), repetitive work (p = 0.004), prolonged standing position (p = 0.007), intense physical effort (p <0.001), lifting heavy loads (p = 0.002), uncomfortable position (p = 0.008) and low social support (p <0.001). The logistic regression analysis showed that intense physical exertion (OR= 7.72, 95% CI: 2.98 - 19.97) and job-strain (OR= 3.24, 95% CI: 2.98 - 19.97) represent risk factors related to MSD. CONCLUSION: A high amount of Tunisian nursing staff in this survey complained of musculoskeletal issues considering the low back pain most often affected. Education courses on prevention and coping approaches for musculoskeletal problems are appropriate for nurses as a way to minimize the rate of work risks and even promote performance patient care.

The P2 promoter of the IGF1 gene is a major epigenetic locus for GH responsiveness.

Short children using growth hormone (GH) to accelerate their growth respond to this treatment with a variable efficacy. The causes of this individual variability are multifactorial and could involve epigenetics. Quantifying the impact of epigenetic variation on response to treatments is an emerging challenge. Here we show that methylation of a cluster of CGs located within the P2 promoter of the insulin-like growth factor 1 (IGF1) gene, notably CG-137, is inversely closely correlated with the response of growth and circulating IGF1 to GH administration. For example, variability in CG-137 methylation contributes 25% to variance of growth response to GH. Methylation of CGs in the P2 promoter is negatively associated with the increased transcriptional activity of P2 promoter in patients' mononuclear blood cells following GH administration. Our observation indicates that epigenetics is a major determinant of GH signaling (physiology) and of individual responsiveness to GH treatment (pharmacoepigenetics).

Technical note: Advantages and limitations of authenticating Palmera goat dairy products by pyrosequencing the melanocortin 1 receptor (MC1R) gene.

Inferring the breed of origin of dairy products can be achieved through molecular analysis of genetic markers with a population-specific pattern of segregation. The goal of the current work was to generate such markers in goats by resequencing several pigmentation genes [melanocortin 1 receptor (MC1R), v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), tyrosinase (TYR), and tyrosinase-related protein 2 (TYRP2)]. This experiment revealed 10 single nucleotide polymorphisms (SNP), including 5 missense mutations and 1 nonsense mutation. These markers were genotyped in 560 goats from 18 breeds originally from Italy, the Iberian Peninsula, the Canary Islands, and North Africa. Although the majority of SNP segregated at moderate frequencies in all populations (including 2 additional markers that were used as a source of information), we identified a c.764G>A SNP in MC1R that displayed highly divergent allelic frequencies in the Palmera breed compared with the Majorera and Tinerfeña breeds from the Canary Islands. Thus, we optimized a pyrosequencing-based technique that allowed us to estimate, very accurately, the allele frequencies of this marker in complex DNA mixtures from different individuals. Once validated, we applied this method to generating breed-specific DNA profiles that made it possible to detect fraudulent cheeses in which Palmero cheese was manufactured with milk from Majorera goats. One limitation of this approach, however, is that it cannot be used to detect illegal manufacturing where Palmero dairy products are produced by mixing milk from Palmera and Majorera goats, because the c.764G>A SNP segregates in both breeds.

[Genotypes of echovirus 6 in Monastir region]. / Génotypes d'échovirus 6 circulant dans la région de Monastir.

UNLABELLED: OBJECTIVE OF THE WORK: Echoviruses of serotype 6 were reported to be endemic in Tunisia and even in other country over the world. they are associated with many outbreak meningitis. The Objective of this study was to genetically characterize echovirus 6 fields isolates. It gives a first approach on the molecular epidemiology of this serotype. MATERIAL AND METHODS: Phylogenetic analysis of partial sequence in the 3'half of the VP1 region (2874-3529) from 25 strains of echovirus 6. RESULTS: 9 genotypes of echovirus 6 were individualized. Study area was Monastir, a touristic tunisian city. Strains were isolated from wastewater during one year, may correspond to three genotypes. CONCLUSION: Many genotype could circulating during the same time and in the same region. This phenomena was reported to be atypic in the case of poliovirus.

A taxonomic reevaluation of Phrynops (Testudines: Chelidae) with the description of two new genera and a new species of Batrachemys.

Relationships among turtle species loosely categorized within the South American genus Phrynops are explored. Three once recognized genera (Batrachemys, Mesoclemmys and Phrynops) that were demoted to subgenera, and then synonymized with Phrynops, are demonstrated to warrant full recognition based on morphometric analysis, skull osteology, and mitochondrial and nuclear gene sequencing. Mesoclemmys is resurrected from the synonymy of Phrynops as a monotypic genus including M. gibba. The genus Rhinemys, previously a synonym of Phrynops, is resurrected for the species R. rufipes. Ranacephala gen. nov. is described to include the species R. hogei. The genus Batrachemys is resurrected from the synonymy of Phrynops and includes B. dahli, B. nasuta, B. raniceps, B. tuberculata, and B. zuliae. The taxon vanderhaegei is placed in Bufocephala gen. nov. The genus Phrynops is redefined to include the taxa P. geoffroanus, P. hilarii, P. tuberosus, and P. williamsi. Cladistic analysis of morphological data supports this taxonomy. A new species of Batrachemys is described from the western Amazon region, and is distinguished by having facial markings in juveniles, a relatively wide head, and a flattened shell. The new species, B. heliostemma sp. nov., is sympatric with and most similar to the recently resurrected form Batrachemys raniceps in the upper Amazonian region of Peru and adjacent Brazil, Ecuador, Venezuela, and Colombia. Lastly, morphometric data from living and museum specimens of all species of Batrachemys are presented.