ABSTRACT
Thiazomycin is a novel thiazolyl peptide closely related to nocathiacin I. It was isolated from Amycolatopsis fastidiosa by chemical and biological screening. Thiazomycin showed highly potent bactericidal activity against Gram-positive pathogens (MIC range 0.002 approximately 0.064 microg/ml) and did not show cross-resistance to clinically relevant antibiotic classes such as beta-lactams, vancomycin, oxazolidinone and quinolones. It was highly efficacious against Staphylococcus aureus infection in mice exhibiting an ED(99) value of 0.15 mg/kg by subcutaneous administration. It inhibited bacterial growth by selective inhibition of protein synthesis and it was thought to interact with L11 protein and 23S rRNA of the 50S ribosome. Structurally, it possesses an oxazolidine ring in the amino-sugar residue that provides further opportunities for selective chemical modifications that are not feasible with other thiazolyl peptides. More importantly such a modification can potentially lead to semi-synthetic compounds that overcome problems that have hampered clinical development of this class of compounds. Despite its positive attributes, emergence of an unacceptable frequency of resistance poses significant challenges for further development of thiazomycin and this class of molecules for therapeutic use.
Subject(s)
Actinomycetales/chemistry , Anti-Bacterial Agents/pharmacology , Peptides, Cyclic/pharmacology , Protein Synthesis Inhibitors/pharmacology , Staphylococcal Infections/drug therapy , Thiazoles/pharmacology , Animals , Anti-Bacterial Agents/isolation & purification , Drug Resistance, Bacterial , Mice , Microbial Sensitivity Tests , Mutation , Peptides/isolation & purification , Peptides/pharmacology , Peptides, Cyclic/isolation & purification , Protein Synthesis Inhibitors/isolation & purification , RNA, Ribosomal/drug effects , Staphylococcus aureus/drug effects , Thiazoles/isolation & purificationABSTRACT
In the late 1960s, the medical need for new antibiotics began to be questioned, and the pharmaceutical industry shifted its emphasis of antibacterials from that of a therapeutic leader to a low-priority research area. Although infectious diseases, in particular those caused by bacterial infections, are still among the top causes of mortality in the world, industrial support continues to wane. The shift from this important area of antimicrobial research has been attributed to a combination of science, medical, marketing and business reasons. This decline in antibacterial drug discovery, coupled with increasing risk as a result of infections caused by drug-resistant bacterial pathogens, represents a clear public health threat.
