Correction: Dissolved organic matter protects mosquito larvae from damaging solar UV radiation.

[This corrects the article DOI: 10.1371/journal.pone.0240261.].

Dissolved organic matter protects mosquito larvae from damaging solar UV radiation.

Mosquitoes have increased in their abundance and geographic distribution in northeastern North America, coinciding with an increase in extreme precipitation events and up to a doubling of dissolved organic matter (DOM) concentrations in some inland waters. Increases in DOM can reduce exposure of mosquito larvae to solar ultraviolet (UV) radiation. Although mosquito larvae are most common in shaded habitats, almost nothing is known about their susceptibility to damage by solar UV radiation, or the ability of DOM to create a refuge from damaging UV in their shallow-water habitats. We hypothesize that 1) exposure to solar UV radiation is lethal to mosquito larvae, 2) larvae lack photo-enzymatic repair to fix UV-damaged DNA, and 3) DOM shades larvae from lethal solar UV radiation. We tested these hypotheses with experiments that manipulated UV radiation, the photo-repair radiation necessary for photo-enzymatic DNA repair, and DOM. Exposure to solar UV radiation significantly decreased larval survivorship, while DOM significantly increased it. There was no evidence of photo-enzymatic DNA repair. Our findings confirm that solar UV radiation decreases habitat suitability for mosquito larvae, but DOM provides a refuge from UV. This highlights the need for vector control managers to prioritize high DOM and shaded habitats in their efforts to reduce mosquito populations.

Shedding light on environmentally transmitted parasites: lighter conditions within lakes restrict epidemic size.

Parasite fitness depends on a successful journey from one host to another. For parasites that are transmitted environmentally, abiotic conditions might modulate the success of this journey. Here we evaluate how light, a key abiotic factor, influences spatiotemporal patterns of zooplankton disease where light varies seasonally, across lakes, and with depth in a lake. In an in situ experiment using those three sources of variation, we tested sensitivity of spores of two parasites to ambient light. Infectivity of both parasites was lower when exposed to ambient light in comparison to parasites exposed to otherwise similar conditions in the dark. The more sensitive parasite (the fungus, Metschnikowia) was damaged even under lower ambient light during late fall (November). With this differential sensitivity established, we evaluated links between light environment and natural outbreaks in lakes. Consistent with the incubations, epidemics of the less sensitive parasite (the bacterium, Pasteuria) started earlier in the fall (under higher ambient light), and both parasites had smaller outbreaks in more transparent lakes. Overall, light environment may impact the timing and size of disease outbreaks. Outbreaks could thus become exacerbated by human activities that darken waters, including lake browning associated with climate change and eutrophication.

Climate change-induced increases in precipitation are reducing the potential for solar ultraviolet radiation to inactivate pathogens in surface waters.

Climate change is accelerating the release of dissolved organic matter (DOM) to inland and coastal waters through increases in precipitation, thawing of permafrost, and changes in vegetation. Our modeling approach suggests that the selective absorption of ultraviolet radiation (UV) by DOM decreases the valuable ecosystem service wherein sunlight inactivates waterborne pathogens. Here we highlight the sensitivity of waterborne pathogens of humans and wildlife to solar UV, and use the DNA action spectrum to model how differences in water transparency and incident sunlight alter the ability of UV to inactivate waterborne pathogens. A case study demonstrates how heavy precipitation events can reduce the solar inactivation potential in Lake Michigan, which provides drinking water to over 10 million people. These data suggest that widespread increases in DOM and consequent browning of surface waters reduce the potential for solar UV inactivation of pathogens, and increase exposure to infectious diseases in humans and wildlife.

Ecological consequences of long-term browning in lakes.

Increases in terrestrially-derived dissolved organic matter (DOM) have led to the browning of inland waters across regions of northeastern North America and Europe. Short-term experimental and comparative studies highlight the important ecological consequences of browning. These range from transparency-induced increases in thermal stratification and oxygen (O2) depletion to changes in pelagic food web structure and alteration of the important role of inland waters in the global carbon cycle. However, multi-decadal studies that document the net ecological consequences of long-term browning are lacking. Here we show that browning over a 27 year period in two lakes of differing transparency resulted in fundamental changes in vertical habitat gradients and food web structure, and that these responses were stronger in the more transparent lake. Surface water temperatures increased by 2-3 °C in both lakes in the absence of any changes in air temperature. Water transparency to ultraviolet (UV) radiation showed a fivefold decrease in the more transparent lake. The primary zooplankton grazers decreased, and in the more transparent lake were largely replaced by a two trophic level zooplankton community. These findings provide new insights into the net effects of the complex and contrasting mechanisms that underlie the ecosystem consequences of browning.

Solar radiation decreases parasitism in Daphnia.

Climate change and variation in atmospheric ozone are influencing the intensity of ultraviolet radiation (UVR) reaching ecosystems. Changing UVR regimes, in turn, may alter epidemics of infectious disease. This possibility hinges on the sensitivity of epidemiologically relevant traits of host and parasite to UVR. We address this issue using a planktonic system (a zooplankton host, Daphnia dentifera, and its virulent fungal parasite, Metschnikowia bicuspidata). Controlled laboratory experiments, coupled with in situ field incubations of spores, revealed that quite low levels of UVR (as well as longer wavelength light) sharply reduced the infectivity of fungal spores but did not affect host susceptibility to infection. The parasite's sensitivity to solar radiation may underlie patterns in a lake survey: higher penetration of solar radiation into lakes correlated with smaller epidemics that started later in autumn (as incident sunlight declined). Thus, solar radiation, by diminishing infectivity of the parasite, may potently reduce disease.