ABSTRACT
The survival of Chinese hamster cell V79-4 after hyperthermic treatment (42 degrees C, 40 minutes) in the exponential growth phase considerably increases with the duration of holding them in the growth medium at 37 degrees C before hypertonic salt treatment (1.5 M NaCl, 15 minutes). The experimental data are interpreted as a recovery of mammalian cells from thermal lesions, whose lethal action manifests itself at high salt concentrations.
Subject(s)
Hot Temperature/adverse effects , Saline Solution, Hypertonic/pharmacology , Sodium Chloride/pharmacology , Animals , Cell Survival/drug effects , Cells, Cultured , Cricetinae , Cricetulus , Temperature , Time FactorsSubject(s)
Carcinoma, Ehrlich Tumor/radiotherapy , Hyperthermia, Induced , Animals , Carcinoma, Ehrlich Tumor/metabolism , Carcinoma, Ehrlich Tumor/mortality , Cell Survival , Cells, Cultured , Combined Modality Therapy , Culture Media/metabolism , Glucose/administration & dosage , Glycolysis , Hydrogen-Ion Concentration , Hyperglycemia/metabolism , Mice , Neoplasm Transplantation , TemperatureABSTRACT
The influence of elevated temperatures (38-41 degrees C) on chromosomes of human lymphocytes on different phases of the cell cycle was studied. A high thermosensitivity of chromosomes was demonstrated during (S + G2)-phases of the cell cycle. There was a significant increase in the number of aberrant cells at t greater than 38.5%. The main types of chromosome aberrations were chromatid and chromosome deletions. Cells with 3-5 aberrations and induction of chromosome aberrations due to breaks in the centromere region were noticed at high temperatures (40-41 degrees).
Subject(s)
Chromosomes, Human/ultrastructure , Lymphocytes/ultrastructure , Temperature , Adult , Cell Cycle , Cells, Cultured , Chromosome Aberrations , Female , Humans , Male , Middle Aged , Time FactorsABSTRACT
Dependence of optic density (lambda = 260 nm) of human lymphocyte nuclear chromatin on temperature under normal conditions and in Down's disease was studied. The low temperature (t approximately 70 degrees C) absorption maximum, absent in the nuclei of lymphocytes in patients with Down's disease, was characteristic of the lymphocyte nuclei of healthy donors. Analysis of the mentioned correlation for individual regions of the nucleus demonstrated the presence of at least two types of regions, i.e. with and without the low temperature absorption maximum. There were more regions of the latter type in Down's disease in comparison with the normal.
