[The features of bulbo-spinal serotoninergic reaction in the young rats under the condition of acute somatic paiN].

Postnatal changes of background and stress-induced (by electric skin nociceptive irritation) concentrations of serotonin (S) and 5-oxyindolacetic acid (5-OIAA)in medulla oblongata and in spinal marrow was investigated using the male white rats (2-4, 17-18 and 30-35 days old). In the intact rats of these three groups the differences of S concentration in medulla oblongata were not revealed. S concentration in spinal marrow and 5-OIAA concentrations in both structures were changed in phase: decreased during vision-appearing period and increased during 1 month age. Rats of three groups showed the following reactions to the pain: 2-4 day rats - increase of spinal pool of S and 5-OIAA and decrease of 5-OIAA fraction in medulla oblongata; 17-18 day rats - hypo-S-shift in both structures, decrease 5-OIAA in spinal marrow and its increase in medulla oblongata; 30-35 day rats - pronounced reduction of both S and 5-OIAA at a bulbo-spinal level. Mechanisms of revealed postnatal changes of S metabolism in CNS and their role in age features of pain sensitivity formation in early ontogenesis were discussed.

[Modeling of the polycystic ovary syndrome].

The objective of the study was to create a model of the polycystic ovary syndrome (PCOS) developing in puberty. 17beta-estradiol 1.5 mg as gel was once applied to the preshaved abdominal skin of 15 female Wistar rats aged 30 days weighing 30-35 g, who had no regular 4-day estrous cycles. Then the animals were fixed for 10-15 minutes until the agent was completely absorbed. For a month, the animals were daily immobilized for 15 minutes. After achieving their sexual maturity, the animals were slaughtered and their ovarian tissue was sampled for histological analysis. The performed study revealed that all 15 (100%) animals had characteristic morphological changes suggesting the development of the polycystic ovary syndrome.

[Adenine nucleotide system in adult and old rat liver during immobilization stress].

Adenine nucleotide and inorganic phosphate concentrations were studied in livers of adult (10-12 month) and old (22-25 month) Wistar male rats during immobilization stress. Study revealed that level of liver energy provision supply of old rats was lower than in adults. Stress caused reduction of adenine nucleotide concentration in the liver of both adult and old animals.

[Age-specific changes of carbohydrate metabolism in rat liver in immobilization stress].

We have studied peculiarities of the main pathways of glucose utilization in the liver of adult and old rats under immobilization stress. Our data indicate that in both adult and old rats stress is accompanied by an increase in glycolysis intensity in the liver. In stressed adult rats stimulation of glycolysis is associated with metabolism changes characteristic of decreased oxidative decarboxylation of pyruvate in the liver. In immobilized old rats glycolysis stimulation took place in maintanence of effective oxidation of pyruvic acid and enhancement of glucose catabolism in pentose phosphate pathway.

[Role of free-radical processes in age-dependent change of energy provision in the liver of rats in stress]. / Rol' svobodnoradikal'nykh protsessov v vozrastnom izmenenii énergeticheskogo obespecheniia pecheni krys pri stresse.

This study was designed to assay the effect of dimethyl sulfoxide to adenine nucleotide and inorganic phosphate concentration in the liver of adult (10-12 month) and old (22-25 month) Wistar male rats during immobilization stress. Investigation have shown that dimethyl sulfoxide injection prevented of AMP concentration decreasing and has no effect to magnitude of ATP decreasing in the both adult and old rats during stress. This antioxidant prevents reduction of total adenine nucleotides level in the liver of old rats during immobilization also. The role of free radical processes stimulation in the adenylate system changes in the liver of adult and old rats during stress have been discussed.

[Comparative characteristics of microcirculation disorders in the serous membrane of the uterus in intact and pregnant rats treated with oxytocin]. / Sravnitel'naia kharakteristika rasstroistv mikrotsirkuliatsii v seroznom pokrove matki intaktnykh i beremennykh krys pri deistvii oksitotsina.

The studies were performed on intact and pregnant rats after the administration of 10(-2) U of oxytocin. Oxytocin-induced increase in spasm duration and microvascular dilatation was noted both in intact and pregnant animals. However, in the former case spasm duration was markedly increased, while in the latter case there was an equal increase both in spasm duration and dilatation. Dilatation phase was characterized by an increase in microvessel diameter, as compared to the initial one. Maximal spasm phase after oxytocin administration in intact rats was characterized by hemostasis. In pregnant animals blood flow was delayed, with no marked spasm present. The initial level of microcirculation was restored 38-40 minutes later.

[Features of microcirculation in the liver during anaphylactic shock]. / Osobennosti mikrotsirkuliatsii v pecheni pri anafilakticheskom shoke

Microcirculation in the liver was studied in rats and dogs with anaphylactic shock. Injection of a reacting dose of the horse serum led to elevation of systemic arterial pressure by an average of 20 mm Hg; there also occurred spasm of the sinusoids, hepatic venules and an acceleration of blood flow in them With the following blood pressure reduction to 60-40 mm Hg there was blood congestion in the vessels, retarded circulation, intravascular aggregation of the blood formed elements, hemorrhages and ischemic changes. Restoration of systemic arterial pressure and of microcirculation occurred in parallel; no complete normalization of microcirculation in the liver ensued, yet.