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1.
Dev Biol ; 462(2): 119-128, 2020 06 15.
Article in English | MEDLINE | ID: mdl-32169553

ABSTRACT

Arl13b is a gene known to regulate ciliogenesis. Functional alterations in this gene's activity have been associated with Joubert syndrome. We found that in Arl13 null mouse embryos the orientation of the optic cup is inverted, such that the lens is abnormally surrounded by an inverted optic cup whose retina pigmented epithelium is oddly facing the surface ectoderm. Loss of Arl13b leads to the disruption of optic vesicle's patterning and expansion of ventral fates. We show that this phenotype is consequence of miss-regulation of Sonic hedgehog (Shh) signaling and demonstrate that the Arl13b-/- eye phenotype can be rescued by deletion of Gli2, a downstream effector of the Shh pathway. This work identified an unexpected role of primary cilia during the morphogenetic movements required for the formation of the eye.


Subject(s)
ADP-Ribosylation Factors/metabolism , Cilia/metabolism , Eye/embryology , ADP-Ribosylation Factors/genetics , Animals , Body Patterning/genetics , Bone Morphogenetic Protein 4/metabolism , Cilia/genetics , Embryonic Development , Eye/metabolism , Eye Proteins/genetics , Eye Proteins/metabolism , Gene Expression Regulation, Developmental/genetics , Hedgehog Proteins/metabolism , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Humans , Lens, Crystalline/embryology , Lens, Crystalline/metabolism , Male , Mice , Mice, Knockout , Morphogenesis , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Organogenesis , Retinal Pigment Epithelium/embryology , Retinal Pigment Epithelium/metabolism , Signal Transduction/genetics , Wnt1 Protein/genetics , Wnt1 Protein/metabolism , Zinc Finger Protein Gli2/genetics , Zinc Finger Protein Gli2/metabolism , Homeobox Protein SIX3
2.
Dev Cell ; 52(3): 350-363.e6, 2020 02 10.
Article in English | MEDLINE | ID: mdl-31928974

ABSTRACT

The mammalian heart contains multiple cell types that appear progressively during embryonic development. Advance in determining cardiac lineage diversification has often been limited by the unreliability of genetic tracers. Here we combine clonal analysis, genetic lineage tracing, tissue transplantation, and mutant characterization to investigate the lineage relationships between epicardium, arterial mesothelial cells (AMCs), and the coronary vasculature. We report a contribution of the second heart field (SHF) to a vasculogenic niche composed of AMCs and sub-mesothelial cells at the base of the pulmonary artery. Sub-mesothelial cells from this niche differentiate into lymphatic endothelial cells and, in close association with AMC-derived cells, contribute to and are essential for the development of ventral cardiac lymphatics. In addition, regionalized epicardial/mesothelial retinoic acid signaling regulates lymphangiogenesis, contributing to the niche properties. These results uncover a SHF vasculogenic contribution to coronary lymphatic development through a local niche at the base of the great arteries.


Subject(s)
Cell Differentiation , Coronary Vessels/physiology , Endothelium, Vascular/physiology , Heart/physiology , Lymphangiogenesis , Lymphatic Vessels/physiology , Pericardium/physiology , Animals , Cell Lineage , Coronary Vessels/cytology , Endothelium, Vascular/cytology , Epithelium/physiology , Female , Heart/embryology , Lymphatic Vessels/cytology , Male , Mice , Pericardium/cytology , Signal Transduction
3.
J Clin Invest ; 129(12): 5489-5500, 2019 12 02.
Article in English | MEDLINE | ID: mdl-31710307

ABSTRACT

Deep venous thrombosis (DVT) and secondary pulmonary embolism cause approximately 100,000 deaths per year in the United States. Physical immobility is the most significant risk factor for DVT, but a molecular and cellular basis for this link has not been defined. We found that the endothelial cells surrounding the venous valve, where DVTs originate, express high levels of FOXC2 and PROX1, transcription factors known to be activated by oscillatory shear stress. The perivalvular venous endothelial cells exhibited a powerful antithrombotic phenotype characterized by low levels of the prothrombotic proteins vWF, P-selectin, and ICAM1 and high levels of the antithrombotic proteins thrombomodulin (THBD), endothelial protein C receptor (EPCR), and tissue factor pathway inhibitor (TFPI). The perivalvular antithrombotic phenotype was lost following genetic deletion of FOXC2 or femoral artery ligation to reduce venous flow in mice, and at the site of origin of human DVT associated with fatal pulmonary embolism. Oscillatory blood flow was detected at perivalvular sites in human veins following muscular activity, but not in the immobile state or after activation of an intermittent compression device designed to prevent DVT. These findings support a mechanism of DVT pathogenesis in which loss of muscular activity results in loss of oscillatory shear-dependent transcriptional and antithrombotic phenotypes in perivalvular venous endothelial cells, and suggest that prevention of DVT and pulmonary embolism may be improved by mechanical devices specifically designed to restore perivalvular oscillatory flow.


Subject(s)
Endothelial Cells/metabolism , Gene Expression Regulation , Hemodynamics/physiology , Venous Thrombosis/prevention & control , Adult , Animals , Female , Forkhead Transcription Factors/physiology , Homeodomain Proteins/physiology , Humans , Male , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Middle Aged , Regional Blood Flow , Tumor Suppressor Proteins/physiology
4.
Development ; 145(17)2018 08 20.
Article in English | MEDLINE | ID: mdl-30042182

ABSTRACT

Although major progress in our understanding of the genes and mechanisms that regulate lymphatic vasculature development has been made, we still do not know how lumen formation and maintenance occurs. Here, we identify the Ras-interacting protein Rasip1 as a key player in this process. We show that lymphatic endothelial cell-specific Rasip1-deficient mouse embryos exhibit enlarged and blood-filled lymphatics at embryonic day 14.5. These vessels have patent lumens with disorganized junctions. Later on, as those vessels become fragmented and lumens collapse, cell junctions become irregular. In addition, Rasip1 deletion at later stages impairs lymphatic valve formation. We determined that Rasip1 is essential for lymphatic lumen maintenance during embryonic development by regulating junction integrity, as Rasip1 loss results in reduced levels of junction molecules and defective cytoskeleton organization in vitro and in vivo We determined that Rasip1 regulates Cdc42 activity, as deletion of Cdc42 results in similar phenotypes to those seen following the loss of Rasip1 Furthermore, ectopic Cdc42 expression rescues the phenotypes in Rasip1-deficient lymphatic endothelial cells, supporting the suggestion that Rasip1 regulates Cdc42 activity to regulate cell junctions and cytoskeleton organization, which are both activities required for lymphatic lumen maintenance.


Subject(s)
Carrier Proteins/metabolism , Cytoskeleton/metabolism , Embryo, Mammalian/embryology , Endothelial Cells/metabolism , Lymphatic Vessels/embryology , Tight Junctions/metabolism , Animals , Carrier Proteins/genetics , Cytoskeleton/genetics , Embryo, Mammalian/cytology , Endothelial Cells/cytology , Intracellular Signaling Peptides and Proteins , Lymphatic Vessels/cytology , Mice , Mice, Transgenic , Tight Junctions/genetics , cdc42 GTP-Binding Protein/genetics , cdc42 GTP-Binding Protein/metabolism
5.
Appl Physiol Nutr Metab ; 42(9): 963-972, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28538106

ABSTRACT

The incidence of type 2 diabetes is highly correlated with obesity; however, there is a lack of research elucidating the temporal progression. Transgenic FVB/N UCP-dta mice, which develop a diabetic phenotype, and their nontransgenic littermates were fed either a high-fat or normal-chow diet and were studied at 6, 9, 12, 15, 18, 21, and 24 weeks of age to test the hypothesis that increased lipid accumulation in skeletal muscle causes mitochondrial dysfunction, leading to the development of insulin resistance. Body composition, intramuscular triglyceride (IMTG) content, glucose metabolism, and mitochondrial function were measured to determine if IMTG drove mitochondrial dysfunction, leading to the development of type 2 diabetes. High-fat-fed transgenic mice had a significantly greater body mass, lipid mass, and IMTG content beginning early in the experiment. Glucose tolerance tests revealed that high-fat-fed transgenic mice developed a significantly insulin resistant response compared with the other 3 groups toward the end of the time course while plasma insulin was elevated very early in the time course. There was no significant difference in several measures of metabolic function throughout the time course. Long-term high-fat feeding in transgenic mice produced increases in IMTG, adiposity, body mass, and plasma insulin accompanied by decreases in glucose metabolism, but did not reveal any deficits in mitochondrial function or regulation during the early stage of the development of type 2 diabetes. It does not appear that lipotoxicity is driving defects in mitochondrial function prior to the onset of insulin resistance.


Subject(s)
Insulin Resistance , Mitochondria, Muscle/metabolism , Muscle, Skeletal/metabolism , Prediabetic State/metabolism , Triglycerides/metabolism , Adiposity , Animals , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/etiology , Diabetes Mellitus, Type 2/metabolism , Diet, High-Fat/adverse effects , Disease Progression , Hyperinsulinism/blood , Hyperinsulinism/etiology , Hyperinsulinism/metabolism , Hyperinsulinism/physiopathology , Male , Mice, Transgenic , Mitochondria, Muscle/enzymology , Mitochondrial Dynamics , Muscle, Skeletal/enzymology , Obesity/blood , Obesity/etiology , Obesity/metabolism , Obesity/physiopathology , Prediabetic State/blood , Prediabetic State/etiology , Prediabetic State/physiopathology , Random Allocation , Reproducibility of Results , Time Factors , Weaning , Weight Gain
6.
Physiol Rep ; 3(4)2015 Apr.
Article in English | MEDLINE | ID: mdl-25896980

ABSTRACT

We investigated the role of muscle activity in maintaining normal glucose homeostasis via transection of the sciatic nerve, an extreme model of disuse atrophy. Mice were killed 3, 10, 28, or 56 days after transection or sham surgery. There was no difference in muscle weight between sham and transected limbs at 3 days post surgery, but it was significantly lower following transection at the other three time points. Transected muscle weight stabilized by 28 days post surgery with no further loss. Myocellular cross-sectional area was significantly smaller at 10, 28, and 56 days post transection surgery. Additionally, muscle fibrosis area was significantly greater at 56 days post transection. In transected muscle there was reduced expression of genes encoding transcriptional regulators of metabolism (PPARα, PGC-1α, PGC-1ß, PPARδ), a glycolytic enzyme (PFK), a fatty acid transporter (M-CPT 1), and an enzyme of mitochondrial oxidation (CS) with transection. In denervated muscle, glucose uptake was significantly lower at 3 days but was greater at 56 days under basal and insulin-stimulated conditions. Although GLUT 4 mRNA was significantly lower at all time points in transected muscle, Western blot analysis showed greater expression of GLUT4 at 28 and 56 days post surgery. GLUT1 mRNA was unchanged; however, GLUT1 protein expression was also greater in transected muscles. Surgery led to significantly higher protein expression for Akt2 as well as higher phosphorylation of Akt. While denervation may initially lead to reduced glucose sensitivity, compensatory responses of insulin signaling appeared to restore and improve glucose uptake in long-term-transected muscle.

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