Impact of the contraction of medial forearm muscles during grip tasks in different forearm positions on medial support at the elbow joint.

We measured the medial joint distance (MJD), activity of muscles involved in medial elbow-joint support, and grip strength, aiming to examine the supportfunction of muscles. MJD was measured in supinated and pronated positions of the forearm of 10 participants under three conditions: at rest (R), under valgus load on the elbow joint (L), and under valgus load on the elbow joint during the grip task (L-grip). Under the L-grip condition, electromyography was performed on flexor digitorum superficialis (FDS), pronator teres (PT), flexor carpi radialis (FCR), and flexor carpi ulnaris (FCU); subsequently, normalized integrated electromyograms (NIEMG) were calculated. Under the L-grip condition, MJD was shorter in the pronated position than in the supinated position (p < 0.001); however, grip strength was lower in the pronated position. NIEMG of FDS was 90% in both positions, and those of FCR and FCU were low at 10%. However, that of PT was 3.6% in the supinated position and 40.9% in the pronated position, showing higher NIEMG in the pronated position (p < 0.001). Medial support during grip tasks was higher in the pronated position probably because PT activity compensates for the decreased FDS activity.

Time-Dependent Changes in the Structure of Calcified Fibrocartilage in the Rat Achilles Tendon-Bone Interface With Sciatic Denervation.

The enthesis transmits a physiological load from soft to hard tissue via fibrocartilage. The histological alterations induced by this physiological loading remain unclear. This study was performed to examine the histomorphological alterations in the collagen fiber bundle alignment and depth of collagen interdigitation between the calcified fibrocartilage and the bone. We examined the Achilles enthesis of rats with sciatic denervation to explore the mechanical effects of structural changes in the enthesis. The parallelism of the collagen fiber bundles was significantly reduced 8 weeks after denervation. However, the depth of collagen interdigitation significantly increased at 2 and 4 weeks after denervation and then significantly decreased 8 weeks after denervation. In conclusion, a lack of muscle loading induced structural alterations in the distal calcified fibrocartilage. These findings suggest that while structural changes in the enthesis are necessary for the development of physiological loading, structural deformities are required in the long term. Anat Rec, 300:2166-2174, 2017. © 2017 Wiley Periodicals, Inc.

Effect of transcranial static magnetic field stimulation over the sensorimotor cortex on somatosensory evoked potentials in humans.

BACKGROUND: The motor cortex in the human brain can be modulated by the application of transcranial static magnetic field stimulation (tSMS) through the scalp. However, the effect of tSMS on the excitability of the primary somatosensory cortex (S1) in humans has never been examined. OBJECTIVE: This study was performed to investigate the possibility of non-invasive modulation of S1 excitability by the application of tSMS in healthy humans. METHODS: tSMS and sham stimulation over the sensorimotor cortex were applied to 10 subjects for periods of 10 and 15 min. Somatosensory evoked potentials (SEPs) following right median nerve stimulation were recorded before and immediately after, 5 min after, and 10 min after tSMS from sites C3' and F3 of the international 10-20 system of electrode placement. In another session, SEPs were recorded from 6 of the 10 subjects every 3 min during 15 min of tSMS. RESULTS: Amplitudes of the N20 component of SEPs at C3' significantly decreased immediately after 10 and 15 min of tSMS by up to 20%, returning to baseline by 10 min after intervention. tSMS applied while recording SEPs every 3 min and sham stimulation had no effect on SEP. CONCLUSIONS: tSMS is able to modulate cortical somatosensory processing in humans, and thus might be a useful tool for inducing plasticity in cortical somatosensory processing. Lack of change in the amplitude of SEPs with tSMS implies that use of peripheral nerve stimulation to cause SEPs antagonizes alteration of the function of membrane ion channels during exposure to static magnetic fields.

Motor cortex-evoked activity in reciprocal muscles is modulated by reward probability.

Horizontal intracortical projections for agonist and antagonist muscles exist in the primary motor cortex (M1), and reward may induce a reinforcement of transmission efficiency of intracortical circuits. We investigated reward-induced change in M1 excitability for agonist and antagonist muscles. Participants were 8 healthy volunteers. Probabilistic reward tasks comprised 3 conditions of 30 trials each: 30 trials contained 10% reward, 30 trials contained 50% reward, and 30 trials contained 90% reward. Each trial began with a cue (red fixation cross), followed by blue circle for 1 s. The subjects were instructed to perform wrist flexion and press a button with the dorsal aspect of middle finger phalanx as quickly as possible in response to disappearance of the blue circle without looking at their hand or the button. Two seconds after the button press, reward/non-reward stimulus was randomly presented for 2-s duration. The reward stimulus was a picture of Japanese 10-yen coin, and each subject received monetary reward at the end of experiment. Subjects were not informed of the reward probabilities. We delivered transcranial magnetic stimulation of the left M1 at the midpoint between center of gravities of agonist flexor carpi radialis (FCR) and antagonist extensor carpi radialis (ECR) muscles at 2 s after the red fixation cross and 1 s after the reward/non-reward stimuli. Relative motor evoked potential (MEP) amplitudes at 2 s after the red fixation cross were significantly higher for 10% reward probability than for 90% reward probability, whereas relative MEP amplitudes at 1 s after reward/non-reward stimuli were significantly higher for 90% reward probability than for 10% and 50% reward probabilities. These results implied that reward could affect the horizontal intracortical projections in M1 for agonist and antagonist muscles, and M1 excitability including the reward-related circuit before and after reward stimulus could be differently altered by reward probability.

Repeated practice of a Go/NoGo visuomotor task induces neuroplastic change in the human posterior parietal cortex: an MEG study.

The posterior parietal cortex (PPC) is strongly related to task performance by evaluating sensory cues and visually guided movements. Sensorimotor processing is improved by task repetition as indicated by reduced response time. We investigated practice-induced changes in PPC visuomotor processing during a Go/NoGo task in humans using 306-channel magnetoencephalography. Eleven healthy adult males were instructed to extend the right index finger when presented with the Go stimulus (a red circle), but not to react to the NoGo stimulus (a green circle or a red square). Magnetic fields over the visual, posterior parietal, and sensorimotor cortices were measured before and after 3 days of task practice. The first peak of the visual-evoked field (VEF) occurred at approximately 80 ms after presentation of either the Go or NoGo stimulus, while a PPC response, with latency to a peak of 175.8 ± 26.7 ms, occurred only after the Go stimulus. No significant change in the first peak of VEF was measured after 3 days of task practice, but there was a significant reduction in the latency to peak PPC activity (160.1 ± 27.6 ms) and in the time from peak PPC activity to electromyogram onset. In all participants, practice resulted in a significant reduction in reaction time. These results demonstrate that practicing a sensorimotor task induces neuroplastic changes in PPC that accelerate sensorimotor processing and reduce motor response times.

Muscle-afferent projection to the sensorimotor cortex after voluntary movement and motor-point stimulation: an MEG study.

OBJECTIVE: To investigate the projection of muscle afferents to the sensorimotor cortex after voluntary finger movement by using magnetoencephalography (MEG). METHODS: The movement-evoked magnetic fields (MEFs) after voluntary index-finger extension were recorded by a 204-channel whole-head MEG system. Somatosensory-evoked magnetic fields (SEFs) were recorded after motor-point stimulation was applied to the right extensor indicis muscle by using a pair of wire electrodes. RESULTS: The MEF waveforms were observed at 35.8±9.7 ms after movement onset (MEF1). The most concentrated SEFs were identified at 78.7±5.6 ms (M70), and the onset latency of M70 was 39.0±5.5 ms after motor-point stimulation. The mean locations of the equivalent current dipoles (ECDs) of MEF1 and M70 were significantly medial and superior to that of N20m elicited by median-nerve stimulation. The ECD locations and directions of both MEF1 and M70 were concordant in the axial, coronal and sagittal planes. CONCLUSIONS: MEF1 and M70 might be elicited by muscle-afferent feedback following muscle contraction. In addition, these ECDs may be located in area 4. SIGNIFICANCE: Motor-point stimulation is a useful tool for confirming the projection of muscle-afferent feedback to the sensorimotor cortex after voluntary movement.

Transcranial direct current stimulation over the motor association cortex induces plastic changes in ipsilateral primary motor and somatosensory cortices.

OBJECTIVE: This study was performed to elucidate whether transcranial direct current stimulation (tDCS) over the motor association cortex modifies the excitability of primary motor (M1) and somatosensory (S1) cortices via neuronal connectivity. METHODS: Anodal, cathodal, and sham tDCS (1 mA) over the left motor association cortex was applied to 10 subjects for 15 min using electrodes of two sizes (9 and 18 cm(2)). Both motor evoked potentials (MEPs) and somatosensory evoked potentials (SEPs) were recorded before, immediately after, and 15 min after tDCS. Electrode positions were confirmed by overlaying them on MRI anatomical surface images of two individuals. RESULTS: After applying anodal tDCS using the large electrode, amplitudes of MEP components significantly decreased, whereas those of early SEP components (N20 and P25) increase. Opposite effects were observed on MEPs and SEPs after cathodal tDCS. However, a small electrode did not significantly influence either MEPs or SEPs, irrespective of polarity. The small electrode covered mainly the dorsal premotor cortex (PMd) while the large electrode involved the supplementary motor area (SMA) in addition to PMd. CONCLUSIONS: These results suggest that anodal tDCS over PMd together with SMA enhanced the inhibitory input to M1 and excitatory input to S1, and that cathodal tDCS might lead to an opposite effect. SIGNIFICANCE: The finding that only the large electrode modulated M1 and S1 implies that activation of PMd together with SMA by tDCS can induce plastic changes in primary sensorimotor areas.

Neuromagnetic activation of primary and secondary somatosensory cortex following tactile-on and tactile-off stimulation.

OBJECTIVE: Magnetoencephalography (MEG) recordings were performed to investigate the cortical activation following tactile-on and tactile-off stimulation. METHODS: We used a 306-ch whole-head MEG system and a tactile stimulator driven by a piezoelectric actuator. Tactile stimuli were applied to the tip of right index finger. The interstimulus interval was set at 2000 ms, which included a constant stimulus of 1000 ms duration. RESULTS: Prominent somatosensory evoked magnetic fields were recorded from the contralateral hemisphere at 57.5 ms and 133.0 ms after the onset of tactile-on stimulation and at 58.2 ms and 138.5 ms after the onset of tactile-off stimulation. All corresponding equivalent current dipoles (ECDs) were located in the primary somatosensory cortex (SI). Moreover, long-latency responses (168.7 ms after tactile-on stimulation, 169.8 ms after tactile-off stimulation) were detected from the ipsilateral hemisphere. The ECDs of these signals were identified in the secondary somatosensory cortex (SII). CONCLUSIONS: The somatosensory evoked magnetic fields waveforms elicited by the two tactile stimuli (tactile-on and tactile-off stimuli) with a mechanical stimulator were strikingly similar. These mechanical stimuli elicited both contralateral SI and ipsilateral SII activities. SIGNIFICANCE: Tactile stimulation with a mechanical stimulator provides new possibilities for experimental designs in studies of the human mechanoreceptor system.

Changes in salivary physiological stress markers induced by muscle stretching in patients with irritable bowel syndrome.

BACKGROUND: Psychophysiological processing has been reported to play a crucial role in irritable bowel syndrome (IBS) but there has been no report on modulation of the stress marker chromogranin A (CgA) resulting from muscle stretching. We hypothesized that abdominal muscle stretching as a passive operation would have a beneficial effect on a biochemical index of the activity of the sympathetic/adrenomedullary system (salivary CgA) and anxiety. METHODS: Fifteen control and eighteen untreated IBS subjects underwent experimental abdominal muscle stretching for 4 min. Subjects relaxed in a supine position with their knees fully flexed while their pelvic and trunk rotation was passively and slowly moved from 0 degrees of abdominal rotation to about 90 degrees or the point where the subject reported feeling discomfort.Changes in the Gastrointestinal Symptoms Rating Scale (GSRS), State Trait Anxiety Inventory (STAI), Self-rating Depression Scale (SDS), ordinate scale and salivary CgA levels were compared between controls and IBS subjects before and after stretching. A three-factor analysis of variance (ANOVA) with period (before vs. after) as the within-subject factor and group (IBS vs. Control), and sex (men vs. female) as the between-subject factors was carried out on salivary CgA. RESULTS: CgA showed significant interactions between period and groups (F[1, 31] = 4.89, p = 0.03), and between groups and sex (F[1, 31] = 4.73, p = 0.03). Interactions between period and sex of CgA secretion were not shown (F[1, 3] = 2.60, p = 0.12). At the baseline, salivary CgA in IBS subjects (36.7 +/- 5.9 pmol/mg) was significantly higher than in controls (19.9 +/- 5.5 pmol/mg, p < 0.05). After the stretching, salivary CgA significantly decreased in the IBS group (25.5 +/- 4.5 pmol/mg), and this value did not differ from that in controls (18.6 +/- 3.9 pmol/mg). CONCLUSION: Our results suggest the possibility of improving IBS pathophysiology by passive abdominal muscle stretching as indicated by CgA, a biochemical index of the activity of the sympathetic/adrenomedullary system.

Cortical neuromagnetic activation accompanying two types of voluntary finger extension.

We examined the amplitude and latency of movement-related cerebral field (MRCF) waveforms, the generator and afferent feedback of movement-evoked field 1 (MEF1), and the relationship between motor field neuromagnetic activity and electromyographic activity during performance of two types of voluntary index extension. Eight healthy, right-handed male volunteers participated in this study. Experiments for each subject consisted of recording of MRCFs following two types of finger movement. One (Task 1) involved voluntary extension of the right index finger to about 40 degrees . In the second (Task 2), an elastic band was placed on the right index fingertip, producing a resistance of about 1.5 times the electromyographic activity associated with the voluntary movement yielding extension to approximately 40 degrees . Peak amplitude and the ECD moment of the motor field differed significantly between the two tasks. In Task 2, the electromechanical delay from EMG onset to movement onset (77.8+/-16.2) was longer than in Task 1 (44.4+/-10.4). However, the latency from EMG onset to MEF1 peak was 87.6+/-8.5 ms in Task 2, and did not differ significantly from that in Task 1 (88.6+/-8.5). The ECDs of MEF1 were located significantly medial to N20 m and lateral and posterior to the motor field. These findings suggest that the ECD of MEF1 is located in area 3b, but is slightly different from N20 m, and that this MEF1 component activation is due not to the onset of joint movement but to that of muscular contraction.

Feasibility of using surface markers for assessing motion of the thumb trapeziometacarpal joint.

OBJECTIVE: The purposes of the present study were to investigate the feasibility of using a skin sensor to represent the first and third metacarpal bone, to verify that there was no significant relative motion between the skin of the third metacarpal bone and trapezium bony segment and to measure thumb metacarpal motion with respect to the hand (third metacarpal). DESIGN: Eight hands from fresh-frozen human cadavers were disarticulated 4 cm proximal to the wrist joint and used in this experiment. BACKGROUND: Recently, magnetic-tracking systems have been developed for the measurement of joint kinematics. Based on the concept of the three-dimensional space within which the thumb metacarpal bone can move, the current study proposes a 3D method for evaluating the relationship between the skin and bony segment. METHODS: An electromagnetic tracking system was used to provide quantitative measurement and evaluation of the relationship between the skin and bony segment while moving the trapeziometacarpal joint. RESULTS: The adjusted coefficient of multiple determination, R(a)(2), values of the kinematic waveforms between the sensors were larger than 0.84. The angular differences and displacements of the center of sensors between the skin and bony sensors were less than 4.9 degrees and 2.8 mm. CONCLUSION: These data revealed that the similarities of the two sensors throughout the motion cycle were high. The differences between the two sensors were also within the clinically allowable range of +/-5 degrees. Therefore, it is feasible to collect motions of the first metacarpal by attaching the skin sensors at the metacarpal head to measure the bony segment.

EMG-angle relationship of the hamstring muscles during maximum knee flexion.

The aim of the present study was to investigate the EMG-joint angle relationship during voluntary contraction with maximum effort and the differences in activity among three hamstring muscles during knee flexion. Ten healthy subjects performed maximum voluntary isometric and isokinetic knee flexion. The isometric tests were performed for 5 s at knee angles of 60 and 90 degrees. The isokinetic test, which consisted of knee flexion from 0 to 120 degrees in the prone position, was performed at an angular velocity of 30 degrees /s (0.523 rad/s). The knee flexion torque was measured using a KIN-COM isokinetic dynamometer. The individual EMG activity of the hamstrings, i.e. the semitendinosus, semimembranosus, long head of the biceps femoris and short head of the biceps femoris muscles, was detected using a bipolar fine wire electrode. With isometric testing, the knee flexion torque at 60 degrees knee flexion was greater than that at 90 degrees. The mean peak isokinetic torque occurred from 15 to 30 degrees knee flexion angle and then the torque decreased as the knee angle increased (p<0.01). The EMG activity of the hamstring muscles varied with the change in knee flexion angle except for the short head of the biceps femoris muscle under isometric condition. With isometric contraction, the integrated EMGs of the semitendinosus and semimembranosus muscles at a knee flexion angle of 60 degrees were significantly lower than that at 90 degrees. During maximum isokinetic contraction, the integrated EMGs of the semitendinosus, semimembranosus and short head of the biceps femoris muscles increased significantly as the knee angle increased from 0 to 105 degrees of knee flexion (p<0.05). On the other hand, the integrated EMG of the long head of the biceps femoris muscle at a knee angle of 60 degrees was significantly greater than that at 90 degrees knee flexion with isometric testing (p<0.01). During maximum isokinetic contraction, the integrated EMG was the greatest at a knee angle between 15 and 30 degrees, and then significantly decreased as the knee angle increased from 30 to 120 degrees (p<0.01). These results demonstrate that the EMG activity of hamstring muscles during maximum isometric and isokinetic knee flexion varies with change in muscle length or joint angle, and that the activity of the long head of the biceps femoris muscle differs considerably from the other three heads of hamstrings.