Celecoxib, a cyclooxygenase-2 inhibitor, offers chemoprevention against reproductive and neurobehavioural abnormalities induced by atrazine in male Wistar rats.

The cyclooxygenase-2/prostanoid pathway (COX-2) serves as a potential therapeutic target in various pathological conditions. Thus, the modulatory effect of celecoxib (CXB), a COX-2 inhibitor, in atrazine-induced toxicity was investigated. Five groups (n = 6 rats per group) of adult male Wistar rats received corn oil (2 ml/kg), atrazine (ATZ, 300 mg/kg) and CXB (5.7 mg/kg) respectively and their combinations via the oral route. Results obtained showed reduced (p < 0.05) sperm motility (25.8%) and counts (27.6%), testosterone (29.9%), luteinizing (33%) and follicle stimulating hormones (78.7%) plus elevated total cholesterol (112.3%), triglyceride (115.7%), malondialdehyde levels respectively in ATZ-treated rats. Similarly, ATZ administration causes reduced locomotion (33.6%), spontaneous motor activity (46.6%) and catalepsy effects (157.3%) respectively. However, CXB divided doses moderately reverse reproductive abnormalities, modulate neurobehavioural deficits and slightly preserved COX-2 elevation following ATZ intoxication. Furthermore, histopathology of testis shows improvement in treated rats. Overall, our data suggest chemopreventive actions via pharmacological inhibition of COX-2 activity during ATZ toxicity.

Effects of aqueous extract of Aspilia africana on reproductive functions of female Wistar rats.

Effects of Aspilia africana leaf extract on oestrous cycle and ovulation were studied in adult female Wistar strain rats. Cyclic female rats weighing 150 to 200 g were divided into two study groups: the oestrous study and ovulation study group. For the oestrous study, the experimental group received 500 mg kg(-1) b. wt. of the extract for 14 days while the control group received distilled water for the same period. In both groups, vaginal lavage was taken daily from the 5th day to monitor the oestrous cycle. For the ovulation study, there was a control group and two experimental groups. The control group received distilled water while group 1 and 2 received 500 and 1000 mg kg(-1) b.wt. of Aspilia africana leaf extract for 16 days, respectively. The animals were sacrificed on the estrous following the treatment. The results showed a significant decrease in the body weight of the treated rats (p = 0.01) and the oestrous cycle was altered after the commencement of extract. This was indicated by the prolonged proestrous and a reduced dioestrus and estrus. There was a dose-dependent reduction in the ovulation s shown by the reduced number of ova observed in the oviduct from the treated rats compared with control (p<0.05). The extract caused inflammation of the fallopian tube, degeneration in the ovarian cortex in the stroma cells of the ovary and disruption of the endometrium of the uterus. Results suggest that aqueous extract of Aspilia africana leaf has antifertility effect by altering oestrous cycle and causing a dose dependent adverse effect on ovulation in Wistar strain rats.

Is hypercalcemic diet a possible antidote to oral contraceptive-induced hypertension?

Administration of oral contraceptive (OC) has been associated with body fluid retention and in high doses over a long period, promotes hypertension. This present investigation tests the hypothesis that the dietary calcium supplementation increases salt and water excretion in OC (norgestre/ethinylestradiol) treated 32 female albino rats randomly distributed into four (1-4) groups of 8 rats each: Control, OC-treated, OC-treated+ Calcium diet fed and Calcium diet fed only respectively. OC was administered to the appropriate groups by gavage. Experimental diet contained 2.5% calcium supplement. Plasma and urinary [Na+] [K+] were evaluated after 8 weeks of experimentation by flame photometry and plasma [Ca2+] by colorimetric method. OC-treatment induced a significant fall in urinary [Na+]. Water excretion was significantly reduced in these animals (control, 3.1±0.56 Vs OC-treated rats, 1.47±0.16). OC-treated rats had significantly higher plasma [K+] compared to control rats. Calcium supplementation induced increases in plasma [Na+], [K+] and augmented urinary Na+ excretion (OC-treated + Ca2+ diet Vs OC-treated only). Compared with the control rats, high Ca2+ diet fed rats exhibited significant increases in plasma [Na+] and [K+] accompanied by significant decreases in urinary H20 excretion. These results strongly suggest that high dietary Ca2+ supplementation increases salt and water excretion in OC-treated rats and potentially moderates fluid retention and blood pressure in these animals, and may be of clinical significance in OC-induced abnormal fluid retention and perhaps OC-induced hypertension.