1.
Anat Histol Embryol
; 20(1): 44-7, 1991 Mar.
Article
in English
| MEDLINE
| ID: mdl-1715135
ABSTRACT
The purpose of this study was to find a combination histochemical staining technique for the evaluation of equine skeletal muscle that is reliable and effective, while offering a substantial reduction in the labor and cost involved with currently used individual histochemical methods. Several combinations under varying conditions of pH were studied. The most uniform results were obtained using an acid preincubation step at an optimal pH of 4.2 followed by reduced nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR) and the remainder of the acid-ATPase procedure.