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1.
Proc Natl Acad Sci U S A ; 98(1): 241-6, 2001 Jan 02.
Article in English | MEDLINE | ID: mdl-11120881

ABSTRACT

The mechanisms controlling induction of anergy at the level of naive CD4+ T cells are poorly understood but thought to reflect limited contact with costimulatory molecules during T cell antigen receptor (TCR) ligation. To clarify this question, naive TCR transgenic CD4+ cells were exposed to specific peptide presented by transfected antigen-presenting cells (APC) expressing MHC class II molecules with defined accessory molecules. Significantly, culturing CD4(+) cells with APC expressing MHC II plus peptide alone elicited early TCR signaling but failed to induce either proliferation or anergy. Culture with APC expressing MHC II plus B7 molecules led to strong proliferation and T cell priming but no anergy. In marked contrast, conspicuous induction of anergy occurred after T cell culture with APC expressing MHC class II and intercellular adhesion molecule-1 (ICAM-1). Thus, at the level of naive CD4(+) cells, anergy induction appears to reflect selective contact with APC expressing ICAM-1 in the absence of B7.


Subject(s)
CD28 Antigens/immunology , CD4-Positive T-Lymphocytes/immunology , Clonal Anergy/immunology , Lymphocyte Function-Associated Antigen-1/immunology , Animals , Antigen-Presenting Cells/immunology , Antigen-Presenting Cells/metabolism , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, CD/metabolism , Antigens, Differentiation, T-Lymphocyte/metabolism , B7-2 Antigen , CD4-Positive T-Lymphocytes/drug effects , Cell Division , Cell Line , Clonal Anergy/drug effects , Drosophila/cytology , Flow Cytometry , Histocompatibility Antigens Class II/genetics , Histocompatibility Antigens Class II/immunology , Intercellular Adhesion Molecule-1/genetics , Intercellular Adhesion Molecule-1/immunology , Interleukins/immunology , Interleukins/pharmacology , Lectins, C-Type , Lymphocyte Activation/drug effects , Lymphocyte Activation/immunology , Membrane Glycoproteins/genetics , Membrane Glycoproteins/immunology , Mice , Mice, Inbred BALB C , Mice, Transgenic , Precipitin Tests , Receptor Aggregation , Receptors, Interleukin-2/metabolism , Transfection
2.
Immunol Res ; 21(2-3): 345-55, 2000.
Article in English | MEDLINE | ID: mdl-10852136

ABSTRACT

Immune responses are by necessity highly regulated to achieve the appropriate balance of aggression and restraint. Among the many factors involved in maintaining this balance are the interactions between accessory molecule receptors expressed on T cells and their ligands on antigen-presenting cells. Our studies during the past several years have focused on defining how particular accessory molecule interactions influence the activation of naïve CD4+ T cells and the subsequent development of effector function. In this article, we discuss our findings on the effects of distinct accessory molecules with particular attention to the unique roles of LFA-1 and CD28 during different phases of the naïve CD4+ cell response.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , Cytotoxicity, Immunologic , Animals , CD28 Antigens/immunology , Humans , Lymphocyte Function-Associated Antigen-1/immunology
3.
J Immunol ; 163(10): 5250-6, 1999 Nov 15.
Article in English | MEDLINE | ID: mdl-10553046

ABSTRACT

During T-APC interactions in vivo, interfering with CD40-CD154 interactions leads to reduced T cell priming, defects in effector function, and, in some cases, T cell tolerance. As shown here, however, presentation of conventional peptide Ags by CD40-deficient spleen APC in vitro leads to normal CD4+ T cell proliferative responses. By contrast, responses to the same peptides presented by purified B cells were markedly reduced in the absence of CD40. Thus, the requirement for CD40-CD154 interactions appears to be strongly influenced by the type of APC involved. Analysis of responses to endogenous superantigens, which are known to be strongly dependent on B cells for presentation, indicated that CD4+ responses to strong Ags are less dependent on CD40 than are responses to weak Ags. Similar findings applied to negative selection in the thymus. Thus, deletion of potentially autoreactive cells depended on CD40 expression when B APC were involved, and this requirement was most pronounced when negative selection was directed to weak Ags.


Subject(s)
Antigen-Presenting Cells/immunology , B-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/immunology , CD40 Antigens/physiology , Lymphocyte Activation , Animals , Antigen-Presenting Cells/cytology , Antigen-Presenting Cells/metabolism , Antigens, Viral/immunology , B-Lymphocytes/cytology , B-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/cytology , CD4-Positive T-Lymphocytes/metabolism , CD40 Antigens/genetics , CD40 Ligand , Dose-Response Relationship, Immunologic , Interphase/immunology , Ligands , Lymphocyte Activation/genetics , Membrane Glycoproteins/immunology , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred AKR , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Peptides/immunology , Spleen/cytology , Spleen/immunology , Superantigens/immunology
4.
Proc Natl Acad Sci U S A ; 96(6): 3023-8, 1999 Mar 16.
Article in English | MEDLINE | ID: mdl-10077630

ABSTRACT

The type of cytokines produced during T cell responses determines susceptibility or resistance to many pathogens and influences the development of autoimmunity and allergy. To define the role of individual accessory molecules in cytokine production during primary immune responses, Drosophila cell lines expressing murine major histocompatibility complex class II molecules with defined combinations of accessory molecules were used to present peptide antigen to naive T cell receptor transgenic T cells. Significantly, expression of B7.1 or B7.2 without additional accessory molecules led to very high production of interleukin (IL)-4, which contrasted with minimal IL-4 production elicited by conventional antigen presenting cells (APC). However, coexpression of ICAM-1 and B7 on Drosophila APC induced little IL-4, suggesting an inhibitory role for intercellular adhesion molecule-1 (ICAM-1). In support of this idea, stimulation of T cell receptor transgenic T cells with peptide presented by splenic APC devoid of ICAM-1 (from ICAM-1-deficient mice) led to high IL-4 production. Thus, the level of IL-4 production by naive CD4(+) T cells during typical primary responses appears to be controlled, at least in part, by T-APC interactions involving ICAM-1.


Subject(s)
Intercellular Adhesion Molecule-1/immunology , Interleukin-4/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocytes/immunology , Animals , Antigen Presentation/genetics , Antigen Presentation/immunology , B7-1 Antigen/immunology , Cell Line , Drosophila , Gene Expression Regulation/immunology , Intercellular Adhesion Molecule-1/genetics , Interleukin-4/genetics , Lymphocyte Activation , Mice , Mice, Transgenic , Receptors, Antigen, T-Cell/genetics , Transfection
5.
J Immunol ; 159(1): 214-21, 1997 Jul 01.
Article in English | MEDLINE | ID: mdl-9200457

ABSTRACT

CD40 ligand (CD40L) expression on T cells is known to play a crucial role in B cell responses. Some evidence also supports a role for CD40L-CD40 interactions in T cell responses, at least in vivo. Whether the T cell requirement for these interactions is an invariable finding, however, is less clear. Here, we provide evidence that the Ag specificity of T cells influences the requirement for CD40L. T cell hybridomas with dual reactivity for two different Ags, allo-H2-Ap and Mls(a) superantigens, display a differential requirement for CD40L expression. Whereas the response to splenic APC expressing Mls(a) Ags requires CD40L expression, the response to alloantigen-bearing APC does not. The requirement for CD40L expression for the Mls(a) response appears to reflect a strong dependence of this response on ICAM-1 (intercellular adhesion molecule-1) and the ability of CD40-mediated signals to regulate ICAM-1 expression. These findings demonstrate that CD40L-CD40-mediated cross-talk is important for some but not all T cell responses and is influenced by both the type of Ag recognized and the type of APC.


Subject(s)
Antibodies, Bispecific/immunology , Antigens, Differentiation, T-Lymphocyte/immunology , CD40 Antigens/immunology , Hybridomas/immunology , Membrane Glycoproteins/immunology , T-Lymphocytes/immunology , Animals , Antibody Specificity , CD40 Ligand , Flow Cytometry , Mice , Superantigens/immunology
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