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1.
Clin Genet ; 81(5): 479-84, 2012 May.
Article in English | MEDLINE | ID: mdl-21391991

ABSTRACT

Hereditary hyperekplexia (HH) is a disorder of the inhibitory glycinergic neurotransmitter system. Mutations in five genes have been reported to cause the disease. However, only single mutation in GLRB, the gene encoding beta-subunit of the glycine receptor, in a singleton patient with HH has been found to date. In this study, 13 patients with HH were identified through neurology and genetic clinics. Formal clinical examinations, linkage analysis, homozygosity mapping, in-mutation screening of GLRB and in silico functional analyses were carried out. A novel mutation in GLRB among nine patients was identified. This c.596 T>G perturbation results in the change of the highly conserved methionine at position 177 to arginine. Besides the classical HH phenotype, seven patients had esotropia and few of them had behavioral problems. This study presents a large family with HH as a result of homozygous mutation in GLRB and expands the clinical spectrum of HH to include eye misalignment disorder. Moreover, the report of these familial cases supports the previous evidence in a single patient of an autosomal recessive inheritance of HH because of defects in GLRB.


Subject(s)
Muscle Rigidity/diagnosis , Muscle Rigidity/genetics , Mutation , Receptors, Glycine/genetics , Adolescent , Adult , Amino Acid Sequence , Base Sequence , Child , Family , Female , Genotype , Humans , Lod Score , Male , Models, Molecular , Molecular Sequence Data , Pedigree , Protein Structure, Secondary , Receptors, Glycine/chemistry , Young Adult
2.
Childs Nerv Syst ; 22(3): 249-52, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16133273

ABSTRACT

GOALS: The aim of this study is to evaluate the benefits of early intervention in two major spondyloepiphyseal dysplasias of Saudi Arabia, namely, multiple sulfatase deficiency (MSD, Austin's disease) and Morquio's disease. The MSD is encountered frequently in the Kingdom and poses significant health risk to the child because of cord compression. The clinics of this hospital have several Austin's patients. RESULTS: This study indicates that early intervention before serious irreversible damage to the cervical cord occurs improves the neurological course of the patient; no patient had a worse outcome. On the other hand, neurosurgical intervention after the neurological symptoms of cord compression occurs was not as rewarding. CONCLUSION: Morquio's disease is more common outside the Kingdom. The results in this study also confirm that early intervention in this disease is beneficial. Other surgeons make a similar recommendation for Morquio's disease. However, their experience with Austin's disease is not reported due to the rarity of this disease elsewhere.


Subject(s)
Mucopolysaccharidosis IV/complications , Multiple Sulfatase Deficiency Disease/complications , Osteochondrodysplasias/surgery , Spinal Cord Compression/surgery , Age Factors , Cervical Vertebrae , Child , Child, Preschool , Decompression, Surgical/methods , Female , Follow-Up Studies , Humans , Male , Odontoid Process/pathology , Osteochondrodysplasias/enzymology , Osteochondrodysplasias/etiology , Saudi Arabia , Spinal Cord Compression/etiology , Spinal Cord Compression/prevention & control , Time Factors , Treatment Outcome
6.
Hum Mutat ; 24(4): 352, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15365997

ABSTRACT

GM1-gangliosidosis is a lysosomal storage disorder caused by a deficiency of beta-galactosidase. It is mainly characterized by progressive neurodegeneration and in its most severe infantile form it leads to death before the age of four. We have performed molecular analysis of five patients with the infantile form of GM1-gangliosidosis originating from the Middle East (two from Saudi Arabia and three from the United Arab Emirates). We have identified four novel mutations and one previously reported mutation in the GLB1 gene. The first novel mutation found in the homoallelic state in a patient from Saudi Arabia, is a c.171C>G transversion in exon 2 which creates a premature stop codon. Northern blot analysis in fibroblasts from the patient showed no mRNA and expression studies in COS-1 cells showed complete absence of the 85kDa precursor protein and no catalytic activity. The second novel mutation is a splicing error in intron 2, c.245+1G>A. This mutation was found in the heteroallelic state in a patient from Saudi Arabia, the second mutation being the previously described c.145C>T mutation. The third novel mutation is a missense mutation in exon 4, c.451G>T, found in the homoallelic state in a patient from the United Arab Emirates. Expression studies of this mutation in COS-1 cells showed complete absence of the 85kDa precursor protein and no catalytic activity. The fourth novel mutation is a splicing mutation in intron 8, c.914+4A>G, found in the homoallelic state in two siblings from the United Arab Emirates. This study has revealed genetic heterogeneity of the beta-galactosidase deficiency in the Arabic population [corrected]


Subject(s)
Gangliosidosis, GM1/genetics , Mutation , beta-Galactosidase/genetics , Animals , COS Cells , Catalysis , Chlorocebus aethiops , Codon, Nonsense , DNA Mutational Analysis , Exons/genetics , Female , Gangliosidosis, GM1/epidemiology , Genetic Heterogeneity , Humans , Introns/genetics , Male , Mutation, Missense , Recombinant Fusion Proteins/metabolism , Saudi Arabia/epidemiology , United Arab Emirates/epidemiology , beta-Galactosidase/deficiency
7.
J Hum Genet ; 45(4): 200-6, 2000.
Article in English | MEDLINE | ID: mdl-10944848

ABSTRACT

To clarify the molecular basis of the late infantile form of galactosialidosis, we characterized a defective protective protein/cathepsin A (PPCA) gene product with the K453E mutation newly found in an Arabic patient with this disease. Immunocytochemical, expression, and metabolic studies revealed that the precursor PPCA was synthesized but not processed to the mature form, and it was degraded in the mutant. A structural model of the mutant PPCA was constructed by amino acid substitution of 453glutamic acid for lysine in the crystal structure of the wild type PPCA precursor reported. The results show that the K453E mutation is located at the dimer interface of the PPCA and reduces the hydrogen bond formation in the dimer. This structural change may cause instability of the PPCA dimer.


Subject(s)
Carboxypeptidases/chemistry , Metabolism, Inborn Errors/enzymology , Neuraminidase/deficiency , beta-Galactosidase/deficiency , Amino Acid Substitution , Blotting, Northern , Carboxypeptidases/deficiency , Carboxypeptidases/genetics , Cathepsin A , Child , Crystallography, X-Ray , Female , Glutamic Acid/chemistry , Humans , Immunohistochemistry , Lysine/chemistry , Metabolism, Inborn Errors/genetics , Models, Molecular , Mutation, Missense , Protein Structure, Quaternary , White People/genetics
8.
Semin Perinatol ; 24(2): 172-93, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10805172

ABSTRACT

A number of organic and amino acidemias, particularly those that involve the oxidation of fatty acids, cause hypoglycemia intermittently. This may be associated with distrubances of acid base equilibrium and accumulation of lactic acid and/or ketone bodies. When such diseases are not diagnosed rapidly, they might lead to neurological crippling and, at times, death. As a group, these disorders involve more than 1 organ and their phenotypic expression may include all or a single system. The symptoms may appear soon after birth or as late as 1 year of age. Their early recognition and rapid intervention provide rewarding clinical outcome. With the recent advances in diagnostic techniques, such as the introduction of tandem mass spectrometry (MS), screening for these diseases now can be performed because rapid identification on a large scale is possible. The phenotypes, mutations involved, pathognomonic laboratory findings, prognosis, and treatment procedures available have been reviewed for major diseases.


Subject(s)
Amino Acid Metabolism, Inborn Errors/complications , Hypoglycemia/etiology , Lipid Metabolism, Inborn Errors/complications , Amino Acids, Branched-Chain/metabolism , Fatty Acids/metabolism , Humans , Infant, Newborn
10.
J Perinatol ; 20(2): 120-8, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10785889

ABSTRACT

The clinical, biochemical, and neuroradiologic findings and clinical follow-up of seven patients with glutaric aciduria type II are reported. Three phenotypes of the disease are encountered: neonatal-onset form with congenital anomalies (two patients) or without congenital anomalies (three patients) and late-onset form (two patients). The neonatal-onset form presents as an overwhelming illness, with severe hypoglycemia and metabolic acidosis leading to rapid death. Frequently it is associated with perinatal energy deprivation, a neonate with low birth weight and prematurity. The late-onset form presents with intermittent episodes of vomiting, hypoglycemia, and acidosis especially after meals rich in fat and/or proteins. All parents are consanguineous and have a first- or second-degree relationship. Initially, in the two phenotypes with neonatal onset and during crisis in the late-onset phenotype, routine laboratory evaluation showed severe metabolic acidosis, with an increased anion gap, hypoglycemia without ketonuria, and disturbed liver function tests. In the majority of patients with neonatal-onset forms, the kidneys, liver, and at times the spleen are enlarged with an increased echogenic pattern; however, no hepatic or renal cysts are detected. Cardiomegaly is observed in most patients. The diagnosis can be easily and rapidly reached through tandem mass spectrometry study of the blood and can further be confirmed by gas chromatography/mass spectrometry analysis of the urine organic acids. In this report, the magnetic resonance imaging/computed tomography brain studies showed brain atrophy, white matter disease, and in one patient, fluid-filled cavities in the periventricular area and putamina. Fluorine-18-labeled 2-fluoro-2-deoxyglucose positron emission tomographic (FDG PET) brain studies in two patients with late-onset disease showed slightly decreased activity in the cerebral cortex in one and in the caudate nuclei in the other. Brain FDG PET scan and magnetic resonance spectroscopy were normal in one patient with neonatal-onset disease. All patients were treated with a diet low in fat and protein, oral riboflavin, and carnitine. The results were promising for the late-onset disease. Intravenous carnitine gave rewarding results in one patient with neonatal-onset disease.


Subject(s)
Acidosis , Glutarates/urine , Metabolism, Inborn Errors/urine , Acidosis/diagnosis , Acidosis/epidemiology , Acidosis/therapy , Age of Onset , Brain/diagnostic imaging , Brain/pathology , Carnitine/analogs & derivatives , Carnitine/cerebrospinal fluid , Carnitine/therapeutic use , Child , Consanguinity , Female , Humans , Infant , Infant, Newborn , Magnetic Resonance Imaging , Male , Mass Spectrometry , Metabolism, Inborn Errors/diagnosis , Metabolism, Inborn Errors/epidemiology , Metabolism, Inborn Errors/therapy , Tomography, Emission-Computed
11.
Brain Dev ; 22(2): 127-31, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10722966

ABSTRACT

We report a five-year-old boy with 4-hydroxybutyric aciduria. The child presented with global developmental delay, severe hypotonia and myoclonic seizures. The urine 4-hydroxybutyric acid was 1038 times that of normal, and other organic acids related to its further metabolism were also increased. Electroencephalography showed findings indicative of cerebral dysfunction. However, other neurophysiological studies were normal. Clinical improvement was observed after the administration of vigabatrin and dextromethorphan. Magnetic resonance imaging of the brain revealed cerebellar vermin atrophy and subtle white matter changes in the cerebral hemispheres. Fluorine-18 labeled 2-fluoro-2-deoxyglucose positron emission tomographic (FDG PET) scan of the brain showed a marked decrease in the cerebellar metabolism, probably related to atrophy of cerebellar vermis and secondary cerebellar deafferentation. FDG PET scan is found to be of value in the understanding and assessment of brain functional alterations. It may be useful in monitoring and optimizing treatment strategies of this rare disease.


Subject(s)
Brain/diagnostic imaging , Brain/pathology , Hydroxybutyrates/urine , Metabolism, Inborn Errors/diagnostic imaging , Metabolism, Inborn Errors/pathology , Child, Preschool , Dextromethorphan/therapeutic use , Fluorodeoxyglucose F18 , Humans , Magnetic Resonance Imaging , Male , Metabolism, Inborn Errors/drug therapy , Saudi Arabia , Tomography, Emission-Computed , Vigabatrin/therapeutic use
12.
Pediatr Neurol ; 22(1): 44-7, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10669205

ABSTRACT

A 2-year, 6-month-old Saudi male with infantile Krabbe's disease was studied with fluorine-18-labeled-2-fluoro-2-deoxyglucose positron emission tomography (FDG PET) scan. The patient presented with a gradual loss of developmental milestones, irritability, and crying. At the advanced stage of the disease, he developed tonic-clonic seizures and became a microcephalic, extremely irritable, blind, spastic quadriplegic child, with no deep tendon reflexes. Laboratory studies revealed normal blood chemistry, muscle enzymes, very long chain fatty acids, and acylcarnitines. No abnormal urinary organic acids were detected. The cerebrospinal fluid protein concentration was increased. Magnetic resonance imaging of the brain revealed mild brain atrophy and white matter disease mainly in the centrum semiovale. Electroretinography was normal; however, electroencephalography and visual-evoked potentials were abnormal. Peripheral nerve conduction studies documented a demyelinating neuropathic process. The FDG PET study of the brain demonstrated a marked decrease in the metabolism of the left cerebral cortex and no uptake in the caudate heads. Normal glucose uptake was observed in the thalami, lentiform nuclei, and cerebellum. The patient did not present for subsequent clinic visits and is presumed dead.


Subject(s)
Leukodystrophy, Globoid Cell/diagnostic imaging , Tomography, Emission-Computed , Child, Preschool , Fluorodeoxyglucose F18 , Humans , Male , Nerve Degeneration/diagnostic imaging , Radiopharmaceuticals
13.
Ann Saudi Med ; 20(3-4): 214-7, 2000.
Article in English | MEDLINE | ID: mdl-17322660

ABSTRACT

BACKGROUND: Fragile X syndrome is the most common cause of inherited mental retardation. Patients with fragile X syndrome show variable mental disability, typical long and narrow facial appearance with large ears and prominent fontanelle and frequent macro-orchidism. It is generally associated with a fragile site at Xq 27.3, which can be observed in the metaphase chromosome following selective culture conditions. At the molecular level, the fragile X syndrome is associated with an amplification of CGG repeat sequence of the FMR1 gene. The prevalence estimates are reported as one per 1500 males and one per 2500 females. Estimated prevalence rates of fragile X syndrome in different ethnic groups range from 0.4-0.8 per 1000 in males and 0.2-0.6 per 1000 in females. In this study, we have determined the frequency of fragile X-positive cases in 305 preselected patients. MATERIALS AND METHODS: Three hundred and five Saudi patients with mental retardation/developmental delay/clinical suspicion of fragile X syndrome were screened for fragile X chromosome by cytogenetic methods. The majority of patients (95.59%) screened were under the age of 20 years. RESULTS: Two hundred and ninety-nine patients (98.03%) were in the category of mild to moderate mental retardation. Twenty-four males (7.86%) and two females (0.65%) were found to express fragile X site at q27.3. The frequency of fragile X-positive cells in males ranged between 7% and 58% (mean 26+/-13.11), while in the females it was between 14% and 21% (mean 12.5+/-35), respectively. CONCLUSION: The frequency of fragile X positive cases found in this study is similar to other reports of fragile X syndrome in preselected patients.

14.
Saudi Med J ; 21(1): 61-71, 2000 Jan.
Article in English | MEDLINE | ID: mdl-11533753

ABSTRACT

OBJECTIVES: To evaluate the clinical, biochemical, neuroradiological, and neurophysiological findings of patients with X-linked adrenoleukodystrophy. METHODS: Retrospective study evaluating the data of 10 X-linked adrenoleukodystrophy patients diagnosed at King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. RESULTS: The common presenting symptoms were deterioration in school performance, vision and hearing, behavioral changes, and seizures. Eight patients survived 1-4 years and one patient 12 years after the initial presentation, while one patient expired. Six patients had the childhood form, 3 had the adolescent form and one had the adrenomyeloneuropathy form. Six are in an advanced stage of the disease and 3 have mild to moderate spasticity. All except 2 manifested moderate to severe dementia with variable degrees of visual loss. Decreased hearing and features of adrenal insufficiency were seen in 7 patients. Very long chain fatty acids were significantly increased in seven and mildly elevated in 2 patients, however the C26 to C22 ratio was increased in all. The characteristic high-signal intensity of parieto-occipital white matter on brain magnetic resonance imaging T2-weighted images was observed in all patients. Two patients had functional study of the brain, which showed hypometabolic activity in gray and white matter of the occipital lobes. Various neurophysiological abnormalities were detected. The response to different treatment modalities was not promising. CONCLUSION: The disease is more common than had been previously recognized due to phenotypic variability and a wide spectrum of presentations. This report describes various aspects of this disorder and emphasizes the importance of early identification and treatment of asymptomatic but biochemically affected individuals, since all current therapeutic approaches are disappointing if overt neurological abnormalities have been already developed.


Subject(s)
Adrenoleukodystrophy/diagnosis , Adrenoleukodystrophy/epidemiology , Adolescent , Adrenoleukodystrophy/blood , Adrenoleukodystrophy/genetics , Child , Child Behavior Disorders/genetics , Child, Preschool , Genetic Variation , Hearing Disorders/genetics , Humans , Intellectual Disability/genetics , Learning Disabilities/genetics , Magnetic Resonance Imaging , Pedigree , Phenotype , Retrospective Studies , Saudi Arabia/epidemiology , Seizures/genetics , Survival Analysis , Tomography, Emission-Computed , Vision Disorders/genetics
15.
Neurosciences (Riyadh) ; 5(1): 35-45, 2000 Jan.
Article in English | MEDLINE | ID: mdl-24276660

ABSTRACT

OBJECTIVE: To evaluate the clinical, biochemical, neuroradiological, and neurophysiological findings of patients with X-linked adrenoleukodystrophy. METHODS: Retrospective study evaluating the data of 10 X-linked adrenoleukodystrophy patients diagnosed at King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. RESULTS: The common presenting symptoms were deterioration in school performance, vision and hearing, behavioral changes, and seizures. Eight patients survived 1-4 years and one patient 12 years after the initial presentation, while one patient expired. Six patients had the childhood form, 3 had the adolescent form and one had the adrenomyeloneuropathy form. Six are in an advanced stage of the disease and 3 have mild to moderate spasticity. All except 2 manifested moderate to severe dementia with variable degrees of visual loss. Decreased hearing and features of adrenal insufficiency were seen in 7 patients. Very long chain fatty acids were significantly increased in seven and mildly elevated in 2 patients, however the C26 to C22 ratio was increased in all. The characteristic high-signal intensity of parieto-occipital white matter on brain magnetic resonance imaging T2-weighted images was observed in all patients. Two patients had functional study of the brain, which showed hypometabolic activity in gray and white matter of the occipital lobes. Various neurophysiological abnormalities were detected. The response to different treatment modalities was not promising. CONCLUSION: The disease is more common than had been previously recognized due to phenotypic variability and a wide spectrum of presentations. This report describes various aspects of this disorder and emphasizes the importance of early identification and treatment of asymptomatic but biochemically affected individuals, since all current therapeutic approaches are disappointing if overt neurological abnormalities have been already developed.

16.
Pediatr Neurol ; 21(5): 826-9, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10593675

ABSTRACT

Pipecolic acid is a biochemical marker frequently detected in group 1 peroxisomal disorders (peroxisomal biogenesis disorders). Its presence, in addition to the constellation of particular phenotypic manifestations and pathologic findings, has led to its recent classification under disorders of peroxisomal biogenesis as a separate disease entity (hyperpipecolic acidemia or hyperpipecolatemia). The clinical, biochemical, and radiologic findings observed in three patients diagnosed with hyperpipecolic acidemia are reported.


Subject(s)
Brain/diagnostic imaging , Peroxisomal Disorders/diagnostic imaging , Pipecolic Acids/urine , Tomography, X-Ray Computed , Child, Preschool , Humans , Infant, Newborn , Male , Peroxisomal Disorders/genetics , Pipecolic Acids/blood
17.
Brain Dev ; 21(8): 559-62, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10598059

ABSTRACT

The clinical, biochemical, pathological and neuroradiological findings of a 2-year-old Saudi boy with infantile G(M1) gangliosidosis are reported. The patient had a progressive neurologic deterioration, manifesting with developmental regression, sensorimotor and psychointellectual dysfunction and generalized spasticity that started at 4 months of age. Cherry-red macula, facial dysmorphia, hepatomegaly, exaggerated startle response to sounds, skeletal dysplasia, and vacuolated foamy lymphocytes that contain finely fibrillar material in addition to lamellar membranes and electron-dense rounded bodies were seen. MRI of the brain demonstrated mild diffuse brain atrophy and features of delayed dysmyelination and demyelination. Brain FDG PET scan revealed a mild decrease in the basal ganglia uptake, and moderate to severe decrease in thalamic and visual cortex uptake, and an area of increased glucose uptake in the left frontal lobe, probably representing an active seizure focus. The functional changes indicated by FDG PET scan and the structural abnormalities shown on MRI were found to be complementary in the imaging evaluation of infantile G(M1) gangliosidosis.


Subject(s)
Fluorodeoxyglucose F18 , Gangliosidosis, GM1/diagnostic imaging , Gangliosidosis, GM1/pathology , Brain/diagnostic imaging , Brain/pathology , Humans , Infant , Lymphocytes/diagnostic imaging , Lymphocytes/pathology , Magnetic Resonance Imaging , Male , Tomography, Emission-Computed
19.
Clin Genet ; 55(6): 444-9, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10450861

ABSTRACT

Pyroglutamic aciduria (5-oxoprolinuria) is a rare autosomal recessive disorder caused by either glutathione synthetase deficiency (GSSD) or 5-oxoprolinase deficiency. GSSD results in low glutathione levels in erythrocytes and may present with hemolytic anemia alone or together with pyroglutamic aciduria, metabolic acidosis, and CNS damage. Five patients with pyroglutamic aciduria were studied. All presented with hemolytic anemia and metabolic acidosis. Two (brothers) also had Fanconi nephropathy, which is not seen in pyroglutamic aciduria. Molecular analyses of the GSS gene was performed in 3 patients. RT-PCR and heteroduplex analysis identified a homozygous deletion in 1 patient and a homozygous mutation in 2 others (brothers with Fanconi nephropathy). Sequencing of glutathione synthetase (GSS) cDNA from the first patient showed a 141-bp deletion corresponding to the entire exon 4, whilst the corresponding genomic DNA showed a G491 --> A homozygous splice site mutation. Sequencing of GSS cDNA from the Fanconi nephropathy patients showed a C847 --> T [ARG283 --> CYS] mutation in exon 9.


Subject(s)
Glutathione Synthase/deficiency , Female , Gas Chromatography-Mass Spectrometry , Gene Deletion , Genes, Recessive , Glutathione Synthase/genetics , Humans , Infant , Infant, Newborn , Male , Point Mutation , Reverse Transcriptase Polymerase Chain Reaction
20.
Eur J Paediatr Neurol ; 3(3): 125-7, 1999.
Article in English | MEDLINE | ID: mdl-10461568

ABSTRACT

We report a 2-year-old boy with ethylmalonic aciduria and vasculopathy syndrome evaluated by 18fluoro-2-deoxyglucose positron emission tomographic (18FDG PET) brain scan, with intense uptake of 18FDG in the caudate nucleus and putamen bilaterally but with no morphological changes on magnetic resonance imaging (MRI). A repeat 18FDG PET brain scan 1 year later showed a significant bilateral decreased uptake of glucose in the putamen and the head of the caudate nucleus as well as a decreased uptake in the frontal lobes. On MRI, there was atrophy and watershed infarcts in the basal ganglia, explaining the loss of glucose uptake. These results reflect a selective vulnerability of the basal ganglia, their functional derangement, and ultimate degeneration.


Subject(s)
Fluorodeoxyglucose F18 , Malonates/urine , Metabolism, Inborn Errors/diagnostic imaging , Neurodegenerative Diseases/diagnostic imaging , Radiopharmaceuticals , Caudate Nucleus/diagnostic imaging , Child, Preschool , Frontal Lobe/diagnostic imaging , Humans , Magnetic Resonance Imaging , Male , Metabolism, Inborn Errors/diagnosis , Metabolism, Inborn Errors/urine , Neurodegenerative Diseases/diagnosis , Neurodegenerative Diseases/urine , Putamen/diagnostic imaging , Tomography, Emission-Computed
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