A Survey of knowledge, attitude, and practices surrounding antimicrobial use by family dairy farmers to mastitis control.

This study determined the family dairy farmers' knowledge, attitudes, and practices regarding the management strategy of mastitis, antibiotic use, and antimicrobial resistance in dairy farms in Türkiye. A total of 455 dairy farmers participated in face-to-face surveys from the villages of Çanakkale in Türkiye. A total of 62 questions that were divided into 5 categories were asked of the participants. Farmers (252/455; 55.4%) noted that they had not heard of any term about the expression of microbial resistance. They believe that antibiotic residues (156/455; 34,3%) do not pass into the soil and environment with animal wastes, and diseases such as mastitis (173/455; 38%) cannot be cured without antibiotics. Farmers indicated that they used antibiotic therapy in the treatment of udder diseases such as mastitis at a rate of 44.8%. Moreover, most dairy farmers (78.2%) decided to start antibiotic treatment with the decision of the veterinarian. Farmers believe that the barn environment is not as clean as necessary at a rate of 23.5%, and the necessary hygiene conditions are not followed in animal care 23.4% as the cause of mastitis. They highlighted that they detected mastitis from the redness or swelling of the udder at a rate of 38.2%. 55.8% of the farmers participating in the study had undergone analysis for the diagnosis of mastitis disease. On the other hand, 57.4% of farmers tried natural products such as olive oil, soft soap, vinegar, clay soil, honey, soapy, and yogurt, instead of antibiotics treatment for mastitis diseases. They have been relying mostly on veterinarian recommendations for AU. The use of culture and susceptibility test results for antimicrobial selection should be expanded among veterinarians. All stakeholders should be included in periodic training programs on topics such as AU, AR, preventive treatments for mastitis, vaccination, public health, and environmental health. Surveillance systems are needed to monitor AU and AR in animals, as in human medicine.

Carbapenem resistance and biofilm formation status of Enterobacterales isolated from raw milk via molecular versus phenotypic methods.

Antibiotic resistance genes can easily be transferred between bacteria in the biofilm. In the dairy industry, many bacterial species forming biofilms on the surfaces of equipment are widely reported. The experiments reported in this research paper aimed to investigate the carbapenem resistance and biofilm formation properties of Enterobacterales isolates which are spoilage microorganisms obtained from raw milk. In addition, the study determined that whether there was a relationship between the biofilm formation ability or the protein spectra of these isolates. In this study, ninety-two Enterobacterales isolates collected from 173 raw milk samples were investigated. Initially, the isolates were identified as Citrobacter braakii (n = 18), Citrobacter freundii (n = 12), Enterobacter asburiae (n = 1), Enterobacter cloacae (n = 3), Escherichia coli (n = 10), Hafnia alvei (n = 18), Klebsiella oxytoca (n = 1), Serratia fonticola (n = 24), Serratia liquefaciens (n = 4), and Serratia marcescens (n = 1) using MALDI-TOF MS. As a result, carbapenem resistance was determined in 6.5% of the isolates by CIM test, MHT, and the disk diffusion methods, but none of them had blaOXA-48, blaKPC, blaNDM-1, blaOXA23, blaOXA-58, blaOXA-51, blaVIM, and blaIMP genes. This may be due to the effect of other resistance mechanisms such as porin loss or increased flow pump activity. Furthermore, biofilm formation (weak and moderate) was detected in 97.8% of the Enterobacterales isolates. The mass spectra of the moderate biofilm producer isolate of Serratia spp. and the mass spectra of the weak biofilm producers of E.coli presented similarities.