Evaluation of Anastomosis Efficiency in Arteriovenous Shunts Created by Using Hand-Sewn Microsurgery and Microvascular Anastomotic Coupler Device.

OBJECTIVE: Microsurgery has made great contributions to the advancement of surgery. In parallel with the developments in microsurgery, various techniques have been developed to perfect the technique. Microvascular anastomotic coupler device (MACD) is one of these techniques. The aim of the study was to evaluate the effectiveness of anastomoses created by using hand-sewn microsurgery (HSM) and MACD. METHODS: Twenty male Sprague-Dawley rats weighing 250 to 300 g were divided into 2 groups randomly. Arteriovenous shunt was performed between carotid artery and internal jugular vein with the principles of HSM in the first group (n=10) and by using the 1-mm anastomotic microvascular device in the second group (n=10). Groups were evaluated for anastomose time, success of anastomosis, thrombosis formation, color Doppler ultrasonography, and histopathological features. RESULTS: Anastomotic time was faster with the coupler device compared with HSM technique. Flow rates were found significantly higher in the MACD group. Endothelialization and wall integrity rates were better in MACD group. CONCLUSIONS: Microvascular anastomotic coupler device is faster than HSM. High quality and durability of vascularization, insignificant foreign body reactions are histopathological advantages of MACD.

Use of acellular dermal matrix in peripheral nerve reconstruction: an experimental study on rat sciatic nerve defect.

BACKGROUND: In patients with nerve tissue defects, the use of autologous nerve grafts is the standard method of treatment. Alternatives to autologous, nerve grafts have attracted the attention of reconstructive surgeons. In this study, the results of nerve repairs using acellular dermal matrix (ADM) in an experimental rat sciatic nerve defect model are presented. METHODS: Thirty-six Sprague-Dawley rats were randomized into 5 groups: Group 1: control group, Group 2: negative control group (n = 6), Group 3: autologous nerve graft group (n = 10), Group 4: donor site entubulated with ADM group (n = 10); and Group 5: nerve graft entubulated with ADM group (n = 10). The animals in each group were evaluated for electrophysiologic functions, gastrocnemius muscle weight and histomorphology on the 3rd and 6th month. RESULTS: The compound muscle action potential was observed to be distinctly lower in Groups 3, 4 and 5 in comparison to the control group. In Group 4, the gastrocnemius ratio (GCR) values on the 6th month were statistically significantly lower than the GCR values in Group 3 and Group 5, The histological scores and myelinated axonal counts in Group 5 were statistically significantly higher than the values in Group 3 and Group 4. CONCLUSION: The results of this study showed that wrapping ADM around nerve grafts resulted in better outcomes with respect to nerve healing.

The Effects of Mucoperichondrial Flap Elevation on Septal L-Strut Cartilage: A Biomechanical and Histologic Analysis in a Rabbit Model.

BACKGROUND: The harvesting of septal cartilage following mucoperichondrial flap elevation has almost become a standard step in rhinoplasty. However, the strength of the remaining septum has not yet been evaluated. In the current experimental study of a rabbit rhinoplasty model, the remaining septum following a graft harvest was analyzed both biomechanically and pathologically. METHODS: Forty New Zealand rabbits were classified into four equal groups. Group 1 consisted of the animals in which unilateral elevation of the mucoperichondrial flaps was undertaken before the graft harvest, group 2 consisted of the animals in which bilateral elevation was undertaken, group 3 included the animals where the septum was exposed and left untouched after a bilateral mucosal flap elevation, and group 4 was designated as the control group. Specimens were analyzed under light microscopy for multiple parameters. Biomechanical analyses were performed with a universal testing device at the Department of Engineering, Biomechanical Laboratories, Istanbul Technical University. RESULTS: Biomechanical analysis in terms of maximum tension revealed significant results among the groups (p = 0.008). Although insignificant results were observed overall using a pathologic analysis, the amount of chondrocytes was lower in group 2 than in group 1 (p = 0.099). The amounts of matrix collagen (p = 0.184) and fibrosis were (p = 0.749) higher in group 2 than in group 1. CONCLUSIONS: From these data, the authors conclude that mucoperichondrium integrity plays a crucial role in the biomechanical strength of the septum. More sophisticated studies with further pathologic analysis are required to determine the exact mechanism of strength loss observed with mucoperichondrial flap elevation.

Is there a profit to use the lateral antebrachial cutaneous nerve as a graft source in digital nerve reconstruction?

BACKGROUND: The digital nerves are commonly injured in emergency hand surgery practice. Lateral antebrachial nerve is of the autologous graft options available in forearm for digital nerve reconstruction. In this report, we aimed the evaluation of this nerve as an autologous nerve source for digital nerve repair. PATIENTS AND METHODS: The overall sensorial results of the lateral antebrachial cutaneous nerve grafting and associated donor site morbidity in neglected digital nerve injuries of 15 patients in Zones 1 and 2 were evaluated Average length of the harvested lateral antebrachial cutaneous nerve grafts was 1.81 cm (0.75-3 cm.). RESULTS: Patients have been followed up for 20.7 months in average (range: 9.3-41 months). According to Highet and Sander criteria modified by Mackinnon and Dellon, nine patients were graded as S4, whereas six patients had S3+ values. According to modified ASSH guidelines for stratification of static 2PD results, excellent results were obtained in five patients, good results were achieved in eight patients and moderate results were obtained in two patients. Both the donor and recipient sites were evaluated with Semmes-Weinstein monofilament tests where satisfactory results have been obtained. Only two patients reported minimal cold intolerance at the donor site apart from the mild hypoesthesia noted at the anterolateral aspect of the middle forearm. CONCLUSION: Quite favorable clinical results with minimal donor site sensorial deficiency, anatomical and histomorphological similarity and being available in close location to surgical area brings up a matter to utilization of LABCN for digital nerve reconstruction.