ABSTRACT
Traumatic spinal cord injury (SCI) causes a loss of locomotor function with associated compromise of the musculo-skeletal system. Whole body vibration (WBV) is a potential therapy following SCI, but little is known about its effects on the musculo-skeletal system. Here, we examined locomotor recovery and the musculo-skeletal system after thoracic (T7-9) compression SCI in adult rats. Daily WBV was started at 1, 7, 14 and 28 days after injury (WBV1-WBV28 respectively) and continued over a 12-week post-injury period. Intact rats, rats with SCI but no WBV (sham-treated) and a group that received passive flexion and extension (PFE) of their hind limbs served as controls. Compared to sham-treated rats, neither WBV nor PFE improved motor function. Only WBV14 and PFE improved body support. In line with earlier studies we failed to detect signs of soleus muscle atrophy (weight, cross sectional diameter, total amount of fibers, mean fiber diameter) or bone loss in the femur (length, weight, bone mineral density). One possible explanation is that, despite of injury extent, the preservation of some axons in the white matter, in combination with quadripedal locomotion, may provide sufficient trophic and neuronal support for the musculoskeletal system.
Subject(s)
Musculoskeletal System/pathology , Spinal Cord Compression/pathology , Spinal Cord Compression/therapy , Spinal Cord Injuries/pathology , Spinal Cord Injuries/therapy , Vibration/therapeutic use , Animals , Atrophy , Axons/pathology , Bone and Bones/pathology , Female , Femur/pathology , Hindlimb/physiopathology , Locomotion , Muscle, Skeletal/pathology , Physical Therapy Modalities , Psychomotor Performance , Rats , Rats, Wistar , Recovery of Function , Thoracic Vertebrae/injuriesABSTRACT
Medical imaging techniques require various body positions. Gravity causes changes in the facial soft tissue and acts in different directions according to the position of the head during imaging. The aim of this study was to evaluate the effect of positional changes on the facial soft tissue. The faces of subjects were scanned in the standing, sitting, and supine body positions. Differences in the positions were compared using the root mean square (RMS), mean absolute deviation (MAD), and mean signed distance (MSD). The displacement of 15 midsagittal and 20 bilateral landmarks was evaluated. The RMS, MAD, and MSD values of the sitting-standing comparison were significantly lower than those of the sitting-supine and standing-supine comparisons. There were no significant differences between the sitting-supine and standing-supine comparisons. Sixteen out of 135 measurements (12%) of the midsagittal landmarks and 94 out of 180 (52%) measurements of the bilateral landmarks showed significant displacements among the body positions. These results demonstrate a significant change in the facial soft tissue caused by body position. Furthermore, these data show the different susceptibilities of the facial soft tissue landmarks to the effect of body position along the x, y, and z axes.
Subject(s)
Face/anatomy & histology , Imaging, Three-Dimensional/instrumentation , Posture , Anatomic Landmarks , Female , Gravitation , Healthy Volunteers , Humans , Image Processing, Computer-Assisted , Male , Young AdultABSTRACT
UNLABELLED: Following spinal cord injury (SCI), loss of spinal and supraspinal control results in desynchronisation of detrusor vesicae (parasympathicus) and external urethral sphincter (sympathicus) activity. Despite recovery of lower urinary tract function being a high priority in patients with SCI, effective treatment options are unavailable largely because mechanisms are poorly understood. PURPOSE AND METHODS: We used a clinically relevant model of thoracic SCI compression injury in adult female Wistar rats and confirmed that lesion volumes following severe injuries were significantly greater compared to moderate injuries (p < 0.05). Between 1-9 weeks, we assessed recovery of bladder function as well as return of locomotor function using the Basso, Beattie and Bresnahan (BBB) score. Bladder morphometrics and overall intramural innervation patterns, as assessed with ß-III tubulin immunohistochemistry, were also examined. RESULTS: Despite variability, bladder function was significantly worse following severe compared to moderate compression injury (p < 0.05); furthermore, the degree of bladder and locomotor dysfunction were significantly correlated (r = 0.59; p < 0.05). In addition, at 9 weeks after SCI we saw significantly greater increases in bladder dry weight (p < 0.05) and wall thickness following severe compared to moderate injury as well as increases in intramural axon density (moderate: 3× normal values; severe 5×; both p < 0.05) that also correlated with injury severity (r = 0.89). CONCLUSION: The moderate and severe compression models show consistent and correlated deficits in bladder and locomotor function, as well as in gross anatomical and histopathological changes. Increased intramural innervation may contribute to neurogenic detrusor overactivity and suggests the use of therapeutic agents which block visceromotoric efferents.