Effect of graphite oxide electrochemically exfoliated over a multimode interference filter.

In this work, we study multimodal interference filters with a graphite oxide coating. Use of the multimodal interference filter shows a distinctive peak in the signal spectrum, and when using the exfoliated graphite coated multimodal interference filter, the signal shows different spectral changes, such as the full width at half maximum of the curve, the maximum power, and central wavelength, which indicates that graphite oxide absorbs part of the energy. In addition, microscope observations when a He-Ne laser is passed through the filter confirm that graphite oxide is adhered to the filter.

Optical fiber pH sensor based on a multimode interference device with polymer overlay.

An optical fiber pH sensor based on a multimode interference structure is presented. The sensitive element is a piece of no-core fiber (NCF) coated with a mixture of polyallylamine hydrochloride and polyacrylic acid by a modified layer-by-layer (LbL) self-assembly method. It is experimentally shown that by reducing the diameter of the NCF by chemical etching, the sensitivity is increased from -0.31n m/p H to -2n m/p H. The sensor exhibits a high linear response of 0.997 over a pH range from 5 to 11.3 with a rapid response time lower than 1 s.

Generation, characterization, and experimental analysis of noise-like pulse envelopes with complex shapes.

We present the generation of 41 noise-like pulse (NLP) envelopes with complex shapes using a passively mode-locked, erbium-doped figure-eight fiber laser (EDFEFL). The tuning of each of the complex forms was carried out by varying the polarization state within the laser cavity, using a quarter-wave retarder (QWR2) inside a nonlinear optical loop mirror (NOLM), which is part of the EDFEFL. The position of the retarder plate was identified and recorded for each of the complex shapes. The temporal and spectral characterization was done using the position of the WQR2 wave plate as an independent variable. We present a single-shot analysis of the dynamics for the temporal amplitude, the full width at half-maximum (FWHM), and the root-mean-square (RMS) width for each of the 360 cycles measured for the 41 complex envelopes. We also perform an analysis for the case in which the pulse is completely divided into subpackets. We analyze the corresponding spectral profile for each of the complex forms generated. Finally, we evaluate the performance of the NOLM theoretical model with our experimental results. The wavelength of the NLPs is 1560 nm; the period of the cavity fiber laser is 1.1 ms; and the temporal FWHM is within the range of nanoseconds.

Linear multimode interference fiber temperature sensor using the liquid in glass thermometer principle.

A temperature sensor based on a multimode interference thermometer is designed and fabricated. The operation mechanism is based on the thermal expansion of a specific volume of ethylene glycol contained in a glass bulb that is connected to a capillary of the same material, with a no-core fiber (NCF) inserted and centered into the capillary tube. As the temperature is increased, the liquid is expanded, and the NCF is gradually covered by the liquid, resulting in a peak wavelength shift that is correlated to the temperature variations. A sensitivity of 0.4447 nm/°C and highly linear response with an R2 of 0.99962 are obtained. The advantage of this configuration is that the sensing temperature range can be adjusted by changing either the inner diameter of the capillary tube or the bulb volume. We can also measure negative temperatures by simply modifying the freezing point of the liquid, which demonstrates the viability of the sensor for many applications.

Fabrication and in vitro behavior of dual-function chitosan/silver nanocomposites for potential wound dressing applications.

We report the synthesis and in vitro evaluation of dual-function chitosan-silver nanoparticles (CTS-AgNPs) films with potential applications as wound dressings. We attempted to formulate nanocomposite films with appropriate AgNPs concentrations to simultaneously display antibacterial activity and suitability for cell culture. Nanocomposites were obtained by CTS-mediated in situ chemical reduction of AgNO3. Circular-shape AgNPs (sizes ca. 7-50 nm) well distributed within the CTS matrices were obtained in concentrations from 0.018 to 0.573 wt%. Efficacy (bacteriostatic and bactericidal properties) of CTS-AgNPs films to decrease planktonic and biofilm bacterial growth was AgNPs concentration- and bacteria strain-dependent. Films showed significant antibacterial activity against Gram-negative E. coli and P. aeruginosa and Gram-positive S. aureus. Antibacterial activity against S. epidermidis was moderated. Films suitability for cell culture was characterized using primary human fibroblasts (HF). HF displayed cell viability higher than 90% and the characteristic fusiform morphology of adhered fibroblast upon culture on films with AgNPs concentration ≤ 0.036 wt%. HF cultured on these films also showed positive expression of tropoelastin, procollagen type I and Ki-67, characteristic proteins of extracellular matrix and proliferative cells, respectively. In vitro assays demonstrated that cytocompatibility/antibacterial properties decreased/increased as silver concentration increased, suggesting that CTS-AgNPS nanocomposite films with ≈0.04-0.20 wt% might be considered as potential temporary dual-function wound dressings.

Effect of oxygen tension on bioenergetics and proteostasis in young and old myoblast precursor cells.

In the majority of studies using primary cultures of myoblasts, the cells are maintained at ambient oxygen tension (21% O2), despite the fact that physiological O2 at the tissue level in vivo is much lower (~1-5% O2). We hypothesized that the cellular response in presence of high oxygen concentration might be particularly important in studies comparing energetic function or oxidative stress in cells isolated from young versus old animals. To test this, we asked whether oxygen tension plays a role in mitochondrial bioenergetics (oxygen consumption, glycolysis and fatty acid oxidation) or oxidative damage to proteins (protein disulfides, carbonyls and aggregates) in myoblast precursor cells (MPCs) isolated from young (3-4 m) and old (29-30 m) C57BL/6 mice. MPCs were grown under physiological (3%) or ambient (21%) O2 for two weeks prior to exposure to an acute oxidative insult (H2O2). Our results show significantly higher basal mitochondrial respiration in young versus old MPCs, an increase in basal respiration in young MPCs maintained at 3% O2 compared to cells maintained at 21% O2, and a shift toward glycolytic metabolism in old MPCs grown at 21% O2. H2O2 treatment significantly reduced respiration in old MPCs grown at 3% O2 but did not further repress respiration at 21% O2 in old MPCs. Oxidative damage to protein was higher in cells maintained at 21% O2 and increased in response to H2O2 in old MPCs. These data underscore the importance of understanding the effect of ambient oxygen tension in cell culture studies, in particular studies measuring oxidative damage and mitochondrial function.