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Sci Rep ; 8(1): 9436, 2018 06 21.
Article in English | MEDLINE | ID: mdl-29930304

ABSTRACT

This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin-biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.


Subject(s)
Chromosomes, Human, Y/genetics , Flow Cytometry/methods , High-Throughput Nucleotide Sequencing/methods , Laser Capture Microdissection/methods , Sequence Analysis, DNA/methods , Cells, Cultured , Chromosomes, Human, Y/chemistry , Humans , Male
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