Subject(s)
Cell Cycle , DNA Replication , DNA, Satellite/biosynthesis , Hybrid Cells/metabolism , Interphase , Animals , Chickens , Clone Cells , Cricetinae , Erythrocytes , Female , Kinetics , Nucleic Acid Hybridization , OvaryABSTRACT
The pattern of segregation of hypoxanthine phosphoribosyltransferase (HPRT, E.C. 2.4.2.8) was determined in synchronized Chinese hamster-chick red blood cell hybrids. Three hybrid lines were synchronized at the G1-S boundary. Bromodeoxyuridine pulses were subsequently applied throughout the S phase, and the frequency of the segregant clones was determined. It was found that the segregation of the chicken-specific HPRT phenotype associated with the loss of a chromosome was potentiated by bromodeoxyuridine administered during the first hour following release of the block.
Subject(s)
Cricetinae/genetics , Cricetulus/genetics , Erythrocytes/enzymology , Hybrid Cells/enzymology , Hypoxanthine Phosphoribosyltransferase/genetics , Animals , Bromodeoxyuridine/pharmacology , Cell Cycle , Cell Line , Cells, Cultured , Culture Media , Hypoxanthine Phosphoribosyltransferase/metabolismABSTRACT
The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines. The three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. Bromodeoxyuridine becomes incorporated into the nuclear DNA and increases the spontaneous segregation rate.
