1.
Methods Mol Biol
; 2817: 133-143, 2024.
Article
in English
| MEDLINE
| ID: mdl-38907152
ABSTRACT
Nontargeted single-cell proteomics analysis by mass spectrometry with sample multiplexing utilizing isobaric labeling is often performed using a carrier proteome. The presented protocol describes a targeted approach that replaces the carrier proteome with a set of synthetic peptides from selected proteins, which improves the identification and quantification of these proteins in single human cells.