ABSTRACT
Ultrastructural initial changes study of convoluted proximal tubule after single dose of 154.8 mC/kg (600R), X-ray whole body radiation was performed in 12 C57BL mice. Twenty-four hours later, mitochondria evidenced proeminent changes such as cristolyses, dilatation and vacuolization. Within 72 hours, heterochromatin nuclei increased and vanished 144 hours after X-ray exposure. Such findings suggest that recovery probably happened because convoluted proximal tubules take place, regeneration occurs in a slow rate.
Subject(s)
Kidney Tubules, Proximal/ultrastructure , Animals , Kidney Tubules, Proximal/radiation effects , Male , Mice , Mice, Inbred C57BL , Microscopy, Electron , Radiation Dosage , Time FactorsABSTRACT
We studied the ultrastructure of the jejunal epithelium of six children suffering from acute episodes of gastroenteritis. Ultrastructural alterations of the jejunal mucosa occurred in practically all of the fragments analyzed, although the intensity of the abnormalities observed varied considerably. In most of the patients the alterations were confined to the microvilli, which appeared shortened and tufted in comparison with controls. These ultrastructural alterations are nonspecific and may represent a general response of the intestinal mucosa against different noxious agents. Severe alteration of the epithelial cells was observed in only one patient. In this case the cytoplasm contained multiple vacuoles that may correspond to dilated endoplasmic reticulum. It is hypothesized that the small intestinal lesions observed in these patients may allow penetration of food antigens, resulting in the appearance of food intolerance frequently described in children suffering from acute diarrhea.
Subject(s)
Diarrhea, Infantile/pathology , Intestinal Mucosa/pathology , Jejunum/pathology , Acute Disease , Biopsy , Endoplasmic Reticulum/ultrastructure , Epithelium/ultrastructure , Female , Humans , Infant , Infant, Newborn , Male , Microscopy, Electron , Microvilli/ultrastructure , Vacuoles/ultrastructureABSTRACT
Comparando-se o numero de lisossomas nas celulas do tubulo contorcido proximal de rim de camundongo, antes e apos irradiacao com diferentes doses de exposicao corpo inteiro aos raios X, constatou-se uma diminuicao estatisticamente significante em 72 horas
Subject(s)
Animals , Mice , Kidney Tubules , Lysosomes , RadiationABSTRACT
In both Wistar rats and C-57 BL mice, the thickness of the glomerular basement membrane increases progressively with age. This change, which can be measured in cohorts of manageable size, produces a measurement of biological age--if one accepts the assumption that it correlates with the viability of the organism that exhibit it. Whole-body exposure to x-rays has no observable influence on this age-related change.
Subject(s)
Aging , Kidney Glomerulus/physiology , Animals , Basement Membrane/physiology , Basement Membrane/radiation effects , Basement Membrane/ultrastructure , Kidney Glomerulus/radiation effects , Kidney Glomerulus/ultrastructure , Male , Mice , Mice, Inbred C57BL , Rats , Rats, Inbred Strains , Whole-Body Irradiation/adverse effectsABSTRACT
Estudo ultra-estrutural do intestino delgado foi realizado em seis criancas portadoras de diarreia aguda. Alteracoes ultra-estruturais do intestino delgado ocorreram em praticamente todos os fragmentos analisados, embora a intensidade das anormalidades observadas variasse consideravelmente. Na maioria dos pacientes as alteracoes foram confinadas a regiao das microvilosidades, que se encontravam encurtadas e com tendencia a formacao de tufos.Somente um paciente apresentou alteracoes intensas e extensivas a todas as celulas epiteliais analisadas. As lesoes do intestino delgado observadas nestes pacientes podem constituir a via inicial para penetracao de antigenos alimentares favorecendo o aparecimento de intolerancia alimentar, tao frequentemente descrita em criancas portadoras de diarreia aguda
Subject(s)
Infant, Newborn , Infant , Humans , Male , Female , Diarrhea, Infantile , Intestine, Small , Microscopy, ElectronABSTRACT
Foram estudados 10 caes mantidos em respiracao mecanica e em que se provocou edema pulmonar por administracao de aloxana.Este edema foi quantificado para relacao entre peso do pulmao umido e peso do pulmao seco O valor medio desta relacao foi de 5,94, denotando a presenca de edema. A histopatologia demonstrou a presenca de edema perivascular intersticial e alveolar. A analise destes dados permitiu que se concluisse que o edema iniciou-se na regiao perivascular e peribronquica, atingindo posteriormente o septo e finalmente o alveolo. A microscopia eletronica demonstrou a presenca de liquido de edema no intersticio e espaco alveolar, rico em proteinas confirmando o aumento de permeabilidade microvascular. Edema celular, alteracao na morfologia das mitocondrias e vesiculas de pinocitose foram outros achados morfologicos
Subject(s)
Animals , Dogs , Pulmonary Edema , Capillary Permeability , Lung , AlloxanABSTRACT
Foram estudados os aspectos da ultra-estrutura pulmonar em dois grupos de caes. O primeiro Grupo (Grupo I, 10 caes) foi submetido a instilacao intratraqueal de solucao fisiologica na quantidade de 2 ml/kg. O segundo Grupo (Grupo II, 10 caes), foi submetido a instilacao endotraqueal de solucao de acido cloridrico 0,01 N (pH igual a 2), na quantidade de 2,0 ml/kg. Os caes de ambos os grupos foram anestesiados com pentobarbital sodico, na quantidade de 20 mg/kg e mantidos em ventilacao mecanica durante quatro horas. As alteracoes encontradas nos pulmoes do Grupo I foram discretas e representadas por alteracoes sugestivas de fenomenos osmoticos. As alteracoes encontradas nos pulmoes do Grupo II foram marcantes, representadas por destruicao celular dos pneumocitos tipo I e II, das celulas endoteliais e epiteliais. A presenca de tufos de material fibroso, sem periocidade, possivelmente colageno desnaturado, constitui achado original, nao descrito na literatura
Subject(s)
Pneumonia, AspirationSubject(s)
Embolism, Fat/physiopathology , Intermittent Positive-Pressure Ventilation , Positive-Pressure Respiration , Pulmonary Embolism/physiopathology , Animals , Dogs , Hemodynamics , Lung/pathology , Microscopy, Electron , Oleic Acids , Oxygen Consumption , Pulmonary Embolism/chemically induced , Pulmonary Embolism/pathologyABSTRACT
The authors have studied the ultrastructure of the lung in dogs following experimental hemorrhagic shock. They found intra-alveolar edema, red cells in the alveolar sac, disappearance of the substance of the lamellated osmiophilic bodies and cellular degeneration. Correlation among these alterations and increase of capillary permeability as well as consumption of surfactant secretion were established.
