ABSTRACT
Introduction: Internet of Things (IoT) has dominated various sectors over human effort with its liberal dimensions of innovations. Shielding various utilizations in terms of extensity, IoT integrated with the cloud has obtained a far-reaching spectrum. Backgrounds: Nevertheless, the SARS-CoV-2 has become an imperil, at the present moment causing remarkable demands on health technologies across the globe and gravely snarling the entire world populace. Whilst, the front-runners are striving enormously to uncover this virus, in the event of medications and evolving vaccines, it is also imperative to explore the existing systems dealing with medical emergence, mitigating its spread, and supremely the planning for thwarting this virus. The extant passive face masks provide effective and feasible protection by screening all the air particles entering the nasal passage. Methodology: This paper aims to enucleate a new "smart mask" paradigm for telehealth. As the vital health parameters like temperature, respiratory rate(RR), and heart rate(HR) are being easily affected by this deadly virus, this paper envisions a wearable mask equipped with an active sensor (LM35 temperature sensor) that would continuously monitor these health parameters of the person wearing the mask and provide real-time analysis of the data through the cloud. Result: This proposed methodology also incorporates a vigilant system that would alert the person, if the necessary physical distance is not maintained. Besides, this application provides a person with a detailed record of his health, sending doctors and hospitals for teleconsultation. Conclusion: Experimental results from a functional prototype have proved it a constructive low-cost system.
ABSTRACT
BACKGROUND: To find out an alternative strategy to antibiotic usage against bacterial infection. MATERIALS AND METHODS: The purpose of this study is to describe the quorum-sensing (QS) inhibitory activity of edible plants and fruits against N-acyl-homoserine lactone (AHL)-mediated violacein production in Chromobacterium violaceum and virulence factor expression in Pseudomonas aeruginosa PAO1. RESULTS: Aqueous extracts of Ananas comosus (Bromeliaceae), Musa paradiciaca (Musaceae), Manilkara zapota (Sapotaceae) and Ocimum sanctum (Lamiaceae) were prepared and anti-QS activity of each extract was tested against AHL-mediated phenotypic expressions of C. violaceum and PAO1. Most of these extracts showed significant reduction in AHL-mediated violacein production in C. violaceum as well as pyocyanin pigment, staphylolytic protease, elastase production and biofilm formation in PAO1. However, these extracts were not inhibitory to bacterial growth, revealing that the QS inhibition by the extracts is not related to static or killing effects on the bacteria. CONCLUSIONS: The present study identified the anti-QS activity of A. comosus, M. paradiciaca, M. zapota and O. sanctum. An AHL-inactivating compound from these plant sources can be used as an alternative to antibiotic compounds to prevent AHL-mediated bacterial infection in higher organisms.
