ABSTRACT
It is shown that strains of Vibrio cholerae of serovar O1, biovar eltor, subtype Ogawa, museum strains V. cholerae of serovar O1 and NAG-vibrios (isolated from various sources: sea, river and sewage water, canal water and people) possess identical composition of cell fatty acids with prevailing hexadecenoic, hexadecanoic and octadecenoic acids. Being identical, fatty acid profiles of V. parahaemolyticus and V. alginolyticus, are close to that of V. cholerae differing from the latter mainly by the higher content of dodecanoic acid. Similarity of Aeromonas sp. and Vibrio strains in the fatty acid composition proves phylogenetic relation-ship of these bacteria. Fatty acid composition of Plesiomonas shigelloides cells characterized by the presence of methylenhexadecanoic acid as well as by similarity with Vibrio and Aeromonas by the content of most fatty acids confirms a supposition of R. R. Colwell on the intermediate status of genus Plesiomonas between the families Enterobacteriaceae and Vibrionaceae. Independent of the growth medium, the strains Vibrio. Aeromonas and Plesiomonas preserved a fatty-acid profile, inherent in them, with variations mainly in the content of fatty acids with the odd number of carbon atoms. Allowing for relative stability of fatty acid composition and its peculiarity in certain taxonomic groups of the studied bacteria, the above test may be used as additional objective criterion to identify the representatives of Vibrionaceae family.
Subject(s)
Fatty Acids/analysis , Vibrionaceae/chemistry , Chromatography, Gas , Fresh Water/microbiology , Humans , Seawater/microbiology , Sewage/microbiology , Vibrionaceae/classification , Vibrionaceae/isolation & purification , Water MicrobiologyABSTRACT
Vibrio cholerae strains, serovar O1, biovar eltor, subtype Ogawa, isolated from different sources, V. cholerae classica and NAG vibrios have identical cell fatty acid composition with the prevalence of hexadecenoic, hexadecanoic and octadecenoic acids. V. parahaemolyticus and V. alginolyticus fatty acid profiles, being identical, are similar to V. cholerae profile, differing from it mainly by a higher content of dodecanoic acid. The similarity of the fatty acid profiles of Aeromonas sp. and Vibrio strains is indicative of their phylogenetic relationship. The fatty acid composition of Plesiomonas shigelloides characterized by the presence of methylenehexadecanoic acid and at the same time by its similarity to that of Vibrio and Aeromonas in the content of the majority of fatty acids, confirms the position on the intermediate status of the genus Plesiomonas between the families Enterobacteriaceae and Vibrionaceae.
