First molecular phylogeny of scorpions of the family Buthidae from India.

Scorpions of the family Buthidae are widespread species in India. While studies are available on diversity and distribution of Indian buthid scorpions, no information is available on the phylogenetic relationships among the members of the family, within India and Asia in general. In the current study, we provide the first molecular phylogeny of buthid scorpions from central western India based on the mitochondrial cytochrome oxidase subunit I (COI) gene. Our analysis confirms the current placement of the species, previously assigned to Mesobuthus in the genus Hottentotta. However, the analysis also suggests that the member of this genus from India form a monophyletic group distinct from the members of Hottentotta from Africa. Species of Lychas formed a monophyletic group. Although Orthochirus was nested within the larger clade of buthidae comprising genera such as Androctonus, Buthacus, Buthus and Odontobuthus, the exact phylogenetic placement will require more taxonomic sampling of the known genera of Buthidae. We also show that there is a substantial genetic variation among the populations of medically important scorpion species Hottentotta tamulus, and the genetic distance is linearly correlated with the geographical distance between the populations.

Endemic Asian chytrid strain infection in threatened and endemic anurans of the Northern Western Ghats, India.

The Western Ghats of India harbors a rich diversity of amphibians with more than 77% species endemic to this region. At least 42% of the endemic species are threatened due to several anthropogenic stressors. However, information on amphibian diseases and their impacts on amphibian populations in this region are scarce. We report the occurrence of Batrachochytridium dendrobatidis (Bd), an epidermal aquatic fungal pathogen that causes chytridiomycosis in amphibians, from the Western Ghats. In the current study we detected the occurrence of a native Asian Bd strain from three endemic and threatened species of anurans, Bombay Night Frog Nyctibatrachus humayuni, Leith's Leaping Frog Indirana leithii and Bombay Bubble Nest Frog Raorchestes bombayensis, for the first time from the northern Western Ghats of India based on diagnostic nested PCR, quantitative PCR, DNA sequencing and histopathology. While, the Bd infected I. leithii and R. bombayensis did not show any external symptoms, N. humayuni showed lesions on the skin, browning of skin and sloughing. Sequencing of Bd 5.8S ribosomal RNA gene, and the ITS1 and ITS2 regions, revealed that the current Bd strain is related to a haplotype endemic to Asia. Our findings confirm the presence of Bd in northern Western Ghats and the affected amphibians may or may not show detectable clinical symptoms. We suggest that the significance of diseases as potential threat to amphibian populations of the Western Ghats needs to be highlighted from the conservation point of view.