ABSTRACT
Primary myelofibrosis (PMF) is a hematological malignancy characterized by activation of the JAK/STAT pathway and risk of leukemic transformation. In this study, we generated an induced Pluripotent Stem (iPS) cell line derived from a 65-year old male PMF patient carrying the 5-pb insertion in the CALR gene (CALRins5) and the c.437 Gâ¯>â¯A mutation in the TP53 gene (p.W146X). The newly derived PMF3.17 iPS cell line harbors the original mutations and was characterized as bona fide iPS. Resource table.
Subject(s)
Primary Myelofibrosis/genetics , Tumor Suppressor Protein p53/genetics , Aged , Humans , Induced Pluripotent Stem Cells/metabolism , Male , Mutation , Primary Myelofibrosis/pathologyABSTRACT
Peripheral blood sample was donated by a 61years old female patient diagnosed with acute myeloid leukemia secondary to a primary myelofibrosis harboring the 52-bp deletion in the CALR gene (c.1092_1143del, p.L367fs*46) and the R693X mutation in the ASXL1 gene (c.2077C>T, p.R693X). CD34+ cells were isolated from the sample and subjected to the reprogramming procedure by using the Sendai virus carrying the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc. iPS colonies generated retained the original mutations and displayed all the features of bona fide iPS cells.
Subject(s)
Leukemia, Myeloid, Acute/therapy , Primary Myelofibrosis/therapy , Animals , Cell Differentiation , Cell Line , Female , Humans , Induced Pluripotent Stem Cells/metabolism , Kruppel-Like Factor 4 , Leukemia, Myeloid, Acute/pathology , Male , Middle Aged , Mutation , Primary Myelofibrosis/pathologyABSTRACT
Monosomy 7 arises as a recurrent chromosome aberration in donor cell leukemia after hematopoietic stem cell transplantation. We report a new case of donor cell leukemia with monosomy 7 following HLA-identical allogenic bone marrow transplantation for severe aplastic anemia (SAA). The male patient received a bone marrow graft from his sister, and monosomy 7 was detected only in the XX donor cells, 34 months after transplantation. The patient's bone marrow microenvironment may have played a role in the leukemic transformation of the donor hematopoietic cells.
