ABSTRACT
The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections.
Subject(s)
Acridine Orange , Bacteremia/microbiology , Bacteria/isolation & purification , Blood Culture , Coloring Agents , Bacteremia/diagnosis , Blood Culture/standards , Coloring Agents/chemistry , Gentian Violet , Gram-Negative Bacteria/isolation & purification , Gram-Negative Bacteria/metabolism , Humans , Phenazines , Sensitivity and Specificity , Staining and Labeling/economics , Yeasts/isolation & purification , Yeasts/metabolismABSTRACT
Cladophialophora bantiana, a dematiaceous fungus and a member of the family Phaeohyphomycetes, is primarily a neurotropic fungus causing central nervous system (CNS) infection. We report a case of a well preserved, young adult male presenting with a capsuloganglionic abscess caused by C. bantiana, a rare entity. Diagnosis was made based on the mycology and histopathology findings of the aspirate from the abscess through a burr hole. The patient responded clinically to amphotericin B.
