Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Vet Pathol ; 55(1): 133-143, 2018 01.
Article in English | MEDLINE | ID: mdl-28718360

ABSTRACT

Horses affected by chronic piroplasmosis may develop poor performance and muscle atrophy. Here we investigate the pathological and immunopathological aspects of myopathy occurring in chronic equine piroplasmosis. The study included 16 horses serologically positive for equine piroplasms presenting with clinical signs and supporting serum biochemical evidence of a myopathy. Skeletal muscle was evaluated by histopathology, immunohistochemistry, indirect immunofluorescence, and molecular detection of piroplasms and inflammatory cytokines in skeletal muscle. Histologic lesions included muscle fiber atrophy (100% of cases), degenerative changes (13/16, 81%), and perivascular perimysial and endomysial lymphocytic infiltrates (81% of cases). In 15 cases (94%), muscle fibers had strong immunostaining for major histocompatibility complex classes I and II. T lymphocyte populations were mainly CD3+, CD8+, and CD4+ in equal proportions, with a lower number of CD79α+ cells. The serum from affected horses was tested by indirect immunofluorescence for binding of IgG, IgM, or IgA to sections of normal equine muscle to detect circulating autoantibodies against muscle antigen(s). In all cases, distinct sarcolemmal staining was detected in sections incubated with serum from affected horses, in contrast to sections incubated with phosphate-buffered saline or equine control sera. Reverse transcription polymerase chain reaction (RT-PCR) testing of muscles from affected animals revealed a significant increase of interferon-γ, interleukin-12, and tumor necrosis factor-α gene expression compared to healthy controls. Theileria equi or Babesia caballi was not detected in samples of affected muscle by RT-PCR. Thus, inflammatory myopathy associated with equine piroplasmosis may involve an autoimmune pathogenesis with upregulation of inflammatory cytokines that may cause myofiber atrophy and degeneration.


Subject(s)
Babesiosis/pathology , Horse Diseases/pathology , Myositis/veterinary , Animals , Babesiosis/complications , Female , Fluorescent Antibody Technique, Indirect/veterinary , Horse Diseases/parasitology , Horses , Male , Muscle, Skeletal/parasitology , Muscle, Skeletal/pathology , Myositis/etiology , Myositis/pathology , Reverse Transcriptase Polymerase Chain Reaction/veterinary
2.
Vet J ; 206(1): 54-60, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26257260

ABSTRACT

Sarcopenia, the age related loss of muscle mass and strength, is a multifactorial condition that occurs in a variety of species and represents a major healthcare concern for older adults in human medicine. In veterinary medicine, skeletal muscle atrophy is often observed in dogs as they reach old age, but the process is not well understood. Autophagy is a mechanism for degradation and recycling of cellular constituents and is potentially involved in sarcopenia. The aim of the present study was to evaluate the expression of three markers of autophagy, Beclin 1, LC3 and p62, in muscle wasting of geriatric dogs, to establish whether the levels of autophagy change with increasing age. Muscle biopsies from 25 geriatric dogs were examined and compared with those from five healthy young dogs. Samples from older dogs, assessed by routine histology, histoenzymatic staining and immunohistochemistry, showed evidence of muscle atrophy, sarcoplasmic vacuolisation and mitochondrial alterations. Furthermore, in 80% of the muscle samples from the older dogs, marked intracytoplasmic staining for Beclin 1 and LC3 was observed. Significantly greater expression of LC3 II and Beclin 1, but lower expression of p62, was found by Western blotting, comparing muscle samples from old vs. young dogs. The results of the study suggest that enhanced autophagy might be one of the factors underlying muscle atrophy in dogs as they age.


Subject(s)
Aging , Autophagy/physiology , Dog Diseases/pathology , Muscular Atrophy/veterinary , Up-Regulation/physiology , Animals , Dogs , Female , Male , Muscular Atrophy/pathology
3.
Acta Parasitol ; 60(2): 356-9, 2015 Jun.
Article in English | MEDLINE | ID: mdl-26204007

ABSTRACT

Angiostrongylus vasorum (Nematoda: Angiostrongylidae) infection was detected at post-mortem examination in the pulmonary arteries and hearts of 34/102 (33,3%) of red foxes (Vulpes vulpes) from the Campania Region in southern Italy. Pathological changes consisted of granulomatous interstitial pneumonia caused by larvae and intravascular pulmonary adult nematodes. These changes confirm that angiostrongylosis infection in red foxes has a mainly chronic course, in which the infected host may disperse parasite larvae in the environment over its lifetime. Results suggest that the life cycle of A. vasorum is well established in the red fox in the Campania Region representing a potential infection risk for dogs.


Subject(s)
Angiostrongylus/isolation & purification , Foxes/physiology , Strongylida Infections/veterinary , Animals , Granuloma/parasitology , Granuloma/pathology , Granulomatous Disease, Chronic/parasitology , Granulomatous Disease, Chronic/pathology , Granulomatous Disease, Chronic/veterinary , Heart/parasitology , Histocytochemistry , Italy , Lung/parasitology , Lung/pathology , Lung Diseases, Interstitial/parasitology , Lung Diseases, Interstitial/pathology , Lung Diseases, Interstitial/veterinary , Microscopy , Pulmonary Artery/parasitology , Strongylida Infections/parasitology , Strongylida Infections/pathology
4.
BMC Vet Res ; 10: 236, 2014 Sep 28.
Article in English | MEDLINE | ID: mdl-25262002

ABSTRACT

BACKGROUND: Canine angiostrongylosis is a nematode infection in domestic dogs and wild carnivores. The present report focuses on epidemiological, clinical and histopathological findings in a case of fatal disseminated angiostrongylosis in a dog living in southern Italy and provides data on the extent of the spread of Angiostrongylus vasorum in the same area. CASE PRESENTATION: A 4-year-old female English Setter from the Campania region of southern Italy was referred with a 2-week history of cough and severe respiratory distress that did not respond to antimicrobial therapy. Based on clinical, radiological, echographical and cytological findings (including the presence of larvae), a suspect diagnosis of lungworm infection was performed. After few days the dog died due to progressive clinical aggravation. Complete postmortem examination was conducted within 24 hours from death and samples from lungs, heart, liver, kidney, spleen, stomach and small intestine were fixed in 10% buffered formalin. Grossly, several hemorrhagic foci were observed mostly in the lungs, liver, kidney. Microscopically, the lungs contained numerous, multifocal to coalescing granulomas composed of epitheliod macrophages, multinucleated giant cells and some neutrophils, frequently associated with parasite eggs and larvae. The lungs contained many firm nodules, many adult nematodes approximately 1.5 to 2 cm in length were observed in cut sections and identified as A. vasorum. A subsequent parasitological survey performed with FLOTAC on stray dogs living in the same area showed the presence of A. vasorum larvae in 17 of 1639 stray dogs examined (1.04%). CONCLUSION: This survey provides new data on distribution of A. vasorum and underlines that canine angiostrongylosis should be considered as differential diagnosis in dogs.


Subject(s)
Angiostrongylus/classification , Dog Diseases/parasitology , Strongylida Infections/veterinary , Animals , Anthelmintics/therapeutic use , Dog Diseases/drug therapy , Dog Diseases/pathology , Dogs , Fatal Outcome , Female , Macrolides/therapeutic use , Strongylida Infections/drug therapy , Strongylida Infections/parasitology , Strongylida Infections/pathology
SELECTION OF CITATIONS
SEARCH DETAIL
...