Structural characterisation of the N-glycan moiety of the barnacle settlement-inducing protein complex (SIPC).

Many barnacle species are gregarious and their cypris larvae display a remarkable ability to explore surfaces before committing to permanent attachment. The chemical cue to gregarious settlement behaviour - the settlement-inducing protein complex (SIPC) - is an α(2)-macroglobulin-like glycoprotein. This cuticular protein may also be involved in cyprid reversible adhesion if its presence is confirmed in footprints of adhesive deposited during exploratory behaviour, which increase the attractiveness of surfaces and signal other cyprids to settle. The full-length open-reading frame of the SIPC gene encodes a protein of 1547 amino acids with seven potential N-glycosylation sites. In this study on Balanus amphitrite, glycan profiling of the SIPC via hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-fluorescence) provided evidence of predominantly high mannose glycans (M2-9), with the occurrence of monofucosylated oligomannose glycans (F(6)M2-4) in lower proportions. The high mannose glycosylation found supports previous observations of an interaction with mannose-binding lectins and exogenous mannose increasing settlement in B. amphitrite cypris larvae. Transmission electron microscopy of the deglycosylated SIPC revealed a multi-lobed globular protein with a diameter of ~8 nm. Obtaining a complete structural characterisation of the SIPC remains a goal that has the potential to inspire solutions to the age-old problem of barnacle fouling.

Marine glycobiology: current status and future perspectives.

Glycobiology, which is the study of the structure and function of carbohydrates and carbohydrate containing molecules, is fundamental to all biological systems.Progress in glycobiology has shed light on a range of complex biological processes associated with, for example,disease and immunology, molecular and cellular communication,and developmental biology. There is an established,if rather modest, tradition of glycobiology research in marine systems that has primarily focused on reproduction,biofouling, and chemical communication. The current status of marine glycobiology research is primarily descriptive with very limited progress on structural elucidation and the subsequent definition of precise functional roles beyond a small number of classical examples, e.g., induction of the acrosome reaction in echinoderms. However, with recent advances in analytical instrumentation, there is now the capacity to begin to characterize marine glycoconjugates,many of which will have potential biomedical and biotechnological applications. The analytical approach to glycoscience has developed to such an extent that it has acquired its own "-omics" identity. Glycomics is the quest to decipher the complex information conveyed by carbohydrate molecules--the carbohydrate code or glycocode. Due to the paucity of structural information available, this article will highlight the fundamental importance of glycobiology for many biological processes in marine organisms and will draw upon the best defined systems. These systems therefore may prove genuine candidates for full carbohydrate characterization.