ABSTRACT
One hundred and twenty bacterial isolates, from activated sludge of a treatment plant collecting wastes enriched in ethoxylated nonylphenols, were studied. Sixty isolates were selected on rich medium and 60 on mineral medium containing two nonylphenol ethoxylates as the sole carbon source. Analysis of biodiversity at the species level was performed by comparing the AluI restriction patterns of the 16S ribosomal DNA amplified by PCR from 120 isolates. The rDNA restriction analysis enabled us to cluster the isolates into 15 groups, five of which represented nearly 77% of the community. Phylogenetic analysis of five strains belonging to these main groups made it possible to assign four of them to the genera Acinetobacter, Aeromonas and Shewanella and one to the Proteus group. The analysis of plasmid content showed a high variability and suggested that horizontal gene transfer had taken place at the intraspecific, interspecific and intergeneric levels.
Subject(s)
Ethylene Glycols/metabolism , Gene Transfer, Horizontal , Gram-Negative Bacteria/classification , Gram-Negative Bacteria/genetics , Sewage/microbiology , Culture Media , DNA, Bacterial/analysis , DNA, Bacterial/genetics , DNA, Ribosomal/analysis , DNA, Ribosomal/genetics , Deoxyribonucleases, Type II Site-Specific/metabolism , Gram-Negative Bacteria/isolation & purification , Industrial Waste , Molecular Sequence Data , Phylogeny , Plasmids/genetics , RNA, Ribosomal, 16S , Restriction Mapping , Sequence Analysis, DNA , Sewage/chemistry , Waste Disposal, FluidABSTRACT
In Italian urban areas air pollution from benzene and benzo(a)pyrene-B(a)P--is mostly caused by traffic. The concentration limits in the atmosphere fixed by Italian legislation up to December 31, 1998 expressed as annual means are 15 micrograms/m3 and 2.5 ng/m3 for benzene and B(a)P respectively and, starting from January 1, 1999, 10 micrograms/m3 and 1 ng/m3. In the city of Florence the concentrations detected and expressed as annual means of benzene and B(a)P in an area with heavy traffic (32.1 micrograms/m3 and 3.5 ng/m3), in a densely populated area (9.2 micrograms/m3 and 1.86 ng/m3), and in a city park (6.0 micrograms/m3 and 0.25 ng/m3), suggest a marked progressive reduction in the atmospheric levels of these chemicals with the distance from the main roads. The environmental data obtained from densely populated areas of a number of Italian cities (Firenze, Milano, Roma, Bologna, Bolzano, Pavia, Modena), the only ones that allow evaluation of the health risk, show benzene concentrations ranging from 6.0 to 11.3 micrograms/m3 and B(a)P levels, measured in heavy traffic areas, from 1.0 to 3.5 ng/m3 respectively (annual mean in 1996). The data obtained in the city of Florence show that the population is exposed weekly to average concentrations of 14.3 micrograms/m3 for benzene and 2.0 ng/m3 for B(a)P. These results suggest that, regarding benzene and B(a)P pollution, the situation in Florence is far from being critical but not such as to ensure that long-term exposure is without adverse effects.
