[Comparison of two routine techniques for the evaluation of the susceptibility to antibiotics of strict anaerobic bacteria: E test AB Biodisk and ATB ANA Biomerieux]. / Comparaison de deux techniques permettant d'apprécier la sensibilité aux antibiotiques des anaérobies stricts en routine: E test AB Biodisk et ATB ANA BioMérieux.

A simple, rapid and reliable method that can be used to test individual isolates in routine is needed. Two systems are now marketed in France. As these two methods have not been studied extensively and never compared, the aim of our study was to evaluate them concurrently. For that 56 anaerobes were collected, including 31 Bacteroides fragilis group strains. The MIC's of 10 antibiotics were determined by the reference agar method. All results were further interpreted in SIR categories. Agreements of the results obtained for each method were assessed comparatively to the reference method results. For E Test and ATB ANA respectively, agreements of results were: 93 and 92.8 p. cent, major discrepancies (R/S): 4.2 and 5.7 p. cent, very major discrepancies (S/R): 3.7 and 1.5 p. cent. Clindamycin was involved in one-third of the discrepancies observed with E test. This study shows that E test and ATB ANA demonstrated interesting alternatives for the susceptibility testing of anaerobes.

A new Epstein-Barr virus negative Burkitt's lymphoma derived cell-line. I. Analysis of cell surface markers and abnormal expression of HLA antigens.

CHEV, a new Epstein-Barr virus negative Burkitt's lymphoma derived cell line has been studied. Karyotype analyses demonstrated the t (8; 14) characteristic translocation. Cell surface characterisation of this line showed the presence of mu and chi immunoglobulin chains and beta 2-microglobulin and the absence of the complement receptor. We are unable to detect HLA--A, B alloantigens on the cell surface which was in contrast with an apparently normal expression of these antigens in the cytoplasm.