The "Prisoner's Dilemma" in the Tumor Biology Context: The Warburg Effect, Proliferation Dynamics and Equilibria.

There are numerous theories concerning carcinogenesis. Starting from the Warburg effect, which was one of the first theories concerning the mitochondrial dysfunction in tumor cells. Further on, the "two-hit" theory, where tumors were considered to be the outcome of genetic aberrations or mutations and more specifically of a certain number of "hits" each one resulting in a mutation. One of the main physical problems of biological systems is proliferation. Proliferation brings forwards two main questions: First, under a given population of cells, at time t what will be the precise population at time t+24h (or any other time point)? Second, what are the metabolic strategies followed by tumor cells in order to facilitate for their growth? In the present work we have used experimental data obtained from proliferation experiments of leukemic cells, where cell population and glucose consumption were evaluated. These data were further used to examine whether cells progress through competitive behavior or synergistically. Our results have shown that cells probably progress through a cooperative strategy.

Bioinformatics and Regression Analyses Manifest Tumor-Specific miRNA Expression Dynamics in Pediatric Embryonal Malignancies.

Pediatric Central Nervous System (CNS) neoplasms are the second most prevalent tumors of childhood. Further on, prognosis of this type of neoplasms still remain poor and the comprehension of the etiology and pathogenesis of the disease still remains scarce. Several reports have identified microRNAs as significant molecules in the development of central nervous system tumors and propose that they might compose key molecules underlying oncogenesis. In a previous study we have identified several miRNAs, common to different subtypes of pediatric embryonal CNS malignancies as well as, we have identified miRNAs that manifest significant dynamics with respect to their expression and the neoplasmatic subtype. Overall, 19 tumor cases from children diagnosed with embryonal brain tumors were investigated. As controls, children who suffered a sudden death underwent autopsy and were not present with any brain malignancy were used (13 samples of varying localization). Our experimental approach included microarrays covering 1211 miRNAs, which appeared to manifest tumor-specific dynamics. In conclusion, it appeared that certain miRNAs are neoplasm specific and in particular, their expression manifests linear dynamics. Thus, the investigation of miRNA expression in pediatric embryonal brain tumors might contribute towards the discovery of tumor-specific miRNA signatures, which could potentially afford the identification of gene-specific biomarkers related to diagnosis, prognosis and patient targeted therapy, as well as help us understand oncogenetic dynamics.

Education in health and nursing informatics.

This chapter focused on some of the key issues of Health and Nursing Informatics Education. First an historical overview of Health and Nursing Informatics Education is presented. We also describe briefly the activities in the domain of Nursing Informatics Education across Europe. The Special Projects for Health and Nursing Informatics in Europe are presented. Because of the variety of educational systems, the International Medical Informatics Association (IMIA) felt the need to develop international recommendations in health and medical informatics education. From a nursing informatics perspective, the IMIA recommendations in Nursing Informatics Education are discussed.