ABSTRACT
It is well-known that uremic patients present prolonged bleeding times as a common complication. Factors responsible for this disorder have been extensively investigated. In order to elucidate the possible role of uremic middle molecules as responsible for the bleeding tendency observed in uremia, we have studied the effect on platelet aggregation of middle molecules obtained from uremic plasma by Sephadex and ion-exchange chromatography. Our results show that some purified middle molecular fractions have a specific inhibitory activity on platelet aggregation and suggest an important role for these compounds in the pathogenesis of uremic bleeding.
Subject(s)
Platelet Aggregation , Uremia/blood , Chromatography, Gel , Chromatography, Ion Exchange , Epinephrine/pharmacology , Humans , Reference ValuesABSTRACT
Polyamines, spermine and spermidine in the micromolar range (1-100 mumol/l) increase ADP and epinephrine-induced platelet aggregation in vitro. The possible rôle of polyamines as regulating factors of platelet aggregation in physiological and pathological states is discussed.
