ABSTRACT
Introducción: El estilo de vida juega un rol fundamental en el riesgo de enfermedades cardiovasculares y enfermedades crónicas. Objetivo: Evaluar el efecto del esquema de ayuno intermitente 16/8 frente a las medidas antropométricas, valores de perfil lipídico, presión arterial y riesgo cardiovascular. Método: Estudio analítico longitudinal con intervención. Quince pacientes tratados en el área de nutrición en un centro privado entre enero y octubre del 2020 sometidos al esquema de ayuno intermitente 16/8 por cuatro meses, buscando la reducción de medidas antropométricas, perfil lipídico, presión arterial y/o riesgo cardiovascular. Variables: peso corporal, índice de masa corporal (IMC), índice cintura cadera (ICC), circunferencia abdominal, perfil lipídico, riesgo cardiovascular según Framingham (tabla de Framingham AMR B), índices colesterol total/HDL y LDL/HDL. Resultados: La edad promedio fue 47.1 años, la talla promedio fue 1.66 m., el peso corporal promedio fue 84.3 Kg., el promedio del IMC fue 30.46, el promedio del ICC fue 0.94, el promedio de la circunferencia abdominal fue 99.87, el promedio del colesterol total, HDL y LDL fue 240.06, 43.73 y 153.2 respectivamente, el promedio del riesgo cardiovascular según Framingham fue 0.11% y el promedio de los índices colesterol total/HDL y LDL/HDL fue 5.88 y 3.69. La evaluación de promedios antes y después encontró diferencias significativas (p < 0.05) a los 3 y 4 meses de evaluación. Conclusiones: El esquema de ayuno intermitente mostró reducción significativa de las medidas antropométricas, perfil lipídico y riesgo cardiovascular. Adicionalmente se observó aumento del HDL.
Introduction: Lifestyle is a fundamental role in the risk of cardiovascular diseases and chronic diseases. Objective: To evaluate the effect of the 16/8 intermittent fasting scheme against anthropometric measurements, lipid profile values, blood pressure ââand cardiovascular risk. Method: Longitudinal analytical study with intervention. Fifteen patients treated in the nutrition area in a private center between January and October 2020 submitted to the 16/8 intermittent fasting scheme for four months, seeking to reduce anthropometric measurements, lipid profile, blood pressure and / or cardiovascular risk. Variables: body weight, body mass index (BMI), waist-hip index (WHI), abdominal circumference, lipid profile, cardiovascular risk according to Framingham (Framingham AMR B table), total cholesterol / HDL and LDL / HDL indices. Results: The average age was 47.1 years, the average height was 1.66 m., The average body weight was 84.3 Kg., The average of the BMI was 30.46, the average of the WHI was 0.94, the average of the abdominal circumference was 99.87, the mean total cholesterol, HDL and LDL were 240.06, 43.73 and 153.2 respectively, the mean cardiovascular risk according to Framingham was 0.11% and the mean total cholesterol / HDL and LDL / HDL indices were 5.88 and 3.69. The evaluation of averages before and after found significant differences (p <0.05) at 3 and 4 months of evaluation. Conclusions: The intermittent fasting scheme showed a significant reduction in anthropometric measurements, lipid profile and cardiovascular risk. Additionally, an increase in HDL was observed.
ABSTRACT
TRF1 is a component of the shelterin complex that protects chromosome ends. TRF1 deficiency leads to early embryonic lethality and to severe organ atrophy when deleted in adult tissues. Here we generate a reporter mouse carrying a knock-in eGFP-TRF1 fusion allele to study the role of TRF1 in stem cell biology and tissue homeostasis. We find that eGFP-TRF1 expression in mice is maximal in known adult stem cell compartments and show that TRF1 ensures their functionality. eGFP-TRF1 is highly expressed in induced pluripotent stem cells, uncoupled from the telomere elongation associated with reprogramming. Selection of eGFP-TRF1-high induced pluripotent stem cells correlates with higher pluripotency as indicated by their ability to form teratomas and chimeras. We further show that TRF1 is necessary for both induction and maintenance of pluripotency, and that TRF1 is a direct transcriptional target of Oct3/4.
Subject(s)
Induced Pluripotent Stem Cells/metabolism , Telomeric Repeat Binding Protein 1/metabolism , Adult Stem Cells/cytology , Adult Stem Cells/metabolism , Alleles , Animals , Atrophy , Biomarkers/metabolism , Cell Compartmentation , Cell Nucleus/metabolism , Cellular Reprogramming , Chromosomal Instability , Clone Cells , Fluorescence , Gene Deletion , Gene Knock-In Techniques , Green Fluorescent Proteins/metabolism , Homeodomain Proteins/metabolism , Induced Pluripotent Stem Cells/cytology , Intestine, Small/pathology , Mice , Nanog Homeobox Protein , Octamer Transcription Factor-3/metabolism , Promoter Regions, Genetic/genetics , Protein Binding , Recombinant Fusion Proteins/metabolism , Skin/cytology , Telomere/metabolism , Telomeric Repeat Binding Protein 1/deficiency , Telomeric Repeat Binding Protein 1/geneticsABSTRACT
Yeast Sir2 deacetylase is a component of the silent information regulator (SIR) complex encompassing Sir2/Sir3/Sir4. Sir2 is recruited to telomeres through Rap1, and this complex spreads into subtelomeric DNA via histone deacetylation. However, potential functions at telomeres for SIRT1, the mammalian orthologue of yeast Sir2, are less clear. We studied both loss of function (SIRT1 deficient) and gain of function (SIRT1(super)) mouse models. Our results indicate that SIRT1 is a positive regulator of telomere length in vivo and attenuates telomere shortening associated with aging, an effect dependent on telomerase activity. Using chromatin immunoprecipitation assays, we find that SIRT1 interacts with telomeric repeats in vivo. In addition, SIRT1 overexpression increases homologous recombination throughout the entire genome, including telomeres, centromeres, and chromosome arms. These findings link SIRT1 to telomere biology and global DNA repair and provide new mechanistic explanations for the known functions of SIRT1 in protection from DNA damage and some age-associated pathologies.
Subject(s)
Recombination, Genetic/genetics , Sirtuin 1/genetics , Telomere/metabolism , Acetylation , Aging/genetics , Animals , Centromere/genetics , Centromere/metabolism , Chromosome Fragility/genetics , Chromosomes/genetics , Chromosomes/metabolism , DNA Damage/genetics , DNA Methylation/genetics , Embryo, Mammalian/cytology , Fibroblasts/metabolism , Gene Expression/genetics , Histone Deacetylases/metabolism , Histones/metabolism , Induced Pluripotent Stem Cells/metabolism , Kidney/metabolism , Liver/metabolism , Mice , Mice, 129 Strain , Mice, Inbred C57BL , Mice, Knockout , Protein Binding/genetics , RNA/genetics , Sister Chromatid Exchange/genetics , Telomerase/genetics , Telomerase/metabolism , Telomere/geneticsABSTRACT
Se determinó la composición bioquímica y el índice de condición de la almeja verde (Polymesoda radiata) en el río Bebedero, Guanacaste, Costa Rica, entre agosto de 1992 y julio de 1993. EL peso promedio de carne fresca fue de 12.53+- 3.78 por ciento, con un valor promedio de humedad de 90.10+- 2.14 por ciento. El promedio de los componentes en base seca fue proteínas 78.29 +- 2.15, lípidos 9.52 +- 1 por ciento, carbohidratos 5.78 +- 2.5 por ciento, cenizas 6.43+- 2.1 por ciento. El promedio del índice de condición para la población fue de 9.89 +- 2.14. El promedio del valor calórico fue 4.5 +- 0.11 Kcal/g. Se determinó que el índice de condición, valor calórico y carbohidratos decrecen con la época reproductiva. Los lípidos, proteínas y cenizas no tienen relación con el ciclo reproductivo
