ABSTRACT
The leaf essential oil of Filipendula vulgaris, consisting mainly of salicylaldehyde (68.6%), was screened for its antimicrobial activity by the disk diffusion and microdilution broth assays. The essential oil remarkably inhibited the growth of all of the tested bacteria and fungi. It seems that the antimicrobial nature of F. vulgaris essential oil can be attributed to the synergistic interactions of the compounds constituting the oil rather than to the presence of a single inhibitory agent. A synergy in salicylaldehyde/linalool mixtures was observed with a maximum interaction situated in the range between 60:40 and 80:20 (mol ratio). At this concentration range (at a dose of 1.7 microg/disk) no microbial growth was observed while the respective pure compounds, at the corresponding quantities, are shown to be dramatically less active. The MIC value for the 60:40 mixture was determined to be less that 0.009 mg/ml. In addition, an antagonistic relationship between salicylaldehyde and methyl salicylate was established. The maximum (negative) interaction was shown to correspond approximately to the mixture at the 40:60 (methyl salicylate/salicylaldehyde) mol ratio resulting in the complete loss of activity at the investigated dose.
Subject(s)
Aldehydes/antagonists & inhibitors , Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Filipendula , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Mitosporic Fungi/drug effects , Phytotherapy , Plant Oils/pharmacology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Antifungal Agents/administration & dosage , Antifungal Agents/therapeutic use , Drug Synergism , Humans , Microbial Sensitivity Tests , Plant Leaves , Plant Oils/administration & dosage , Plant Oils/therapeutic useABSTRACT
The authors describe their own system of ultrashort antimicrobial prophylaxis in colorectal surgery. They give an account of two years' experience with their system of ultrashort antimicrobial prophylaxis and demonstrate its effectiveness, feasibility and economic advantages on a group of 113 patients. They implement the prophylaxis of wound infection in colorectal surgery by antibacterial substances which are available in all health facilities in Czechoslovakia. In a group of 113 patients after elective operations of the colon and rectum they recorded, when using their system of ultrashort antimicrobial prophylaxis, 7.07% wound complications.
