Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis.

Antigenic fractions of 100, 50, 37, and 28 kDa obtained through the SDS-PAGE method that were more frequently recognized by anti-Coccidioides antibodies in the sera of coccidioidomycosis patients were selected using western blotting. Subsequently, these bands were sequenced, and the obtained proteins were analysed by BLAST to choose peptides specific for Coccidioides spp. from among the shared aligned sequences of related fungi. A peptide specific for C. immitis was selected from the "GPI anchored serine-threonine rich protein OS C. immitis", while from the "uncharacterized protein of C. immitis", we selected a peptide for C. immitis and C. posadasii. These proteins arose from the 100 kDa antigenic fraction. From the protein "fatty acid amide hydrolase 1 of C. posadasii" that was identified from the 50 kDa antigenic fraction, a peptide was selected that recognized C. immitis and C. posadasii. In addition, the analysis of all the peptides (353) of each of the assembled proteins showed that only 35 had 100% identity with proteins of C. immitis and C. posadasii, one had 100% identity with only C. immitis, and one had 100% identity with only C. posadasii. These peptides can be used as diagnostic reagents, vaccines, and antifungals.

Analysis of partial body weight support during treadmill and overground walking of children with cerebral palsy.

OBJECTIVE: to analyze the spatial-temporal characteristics and joint angles during overground walking without body weight support (BWS) and with 0% and 30% BWS, and during treadmill walking with the same BWS in children with cerebral palsy. METHODS: six children with hemiplegic and spastic cerebral palsy (7.70 ± 1.04 years old) were videotaped during overground walking at a comfortable speed with no BWS, with 0% and 30% BWS, and during treadmill walking with 0% and 30% BWS. Reflective markers were placed over main bony landmarks in both body sides to register the coordinates "x", "y", "z". RESULTS: during overground walking, children walked faster and presented longer and faster strides, longer duration of single-stance and swing periods, and shorter duration of double-stance period, than treadmill walking, regardless of BWS use. The hip was the only joint that presented a difference between body sides and experimental conditions; i.e. range of motion (ROM) was reduced in the plegic side when compared to the nonplegic side, and during overground walking without BWS when compared to 30% BWS. CONCLUSION: children with hemiplegic and spastic cerebral palsy were able to walk overground and on a treadmill with different percentages of BWS, and their performance was superior during overground walking, regardless of BWS use.

Análise do uso de suporte parcial de peso corporal em esteira e em piso fixo durante o andar de crianças com paralisia cerebral / Analysis of partial body weight support during treadmill and overground walking of children with cerebral palsy

OBJETIVO: Analisar características espaço-temporais e ângulos articulares de crianças com paralisia cerebral andando sem o uso de suporte parcial de peso corporal (SPPC) em piso fixo e com 0 por cento e 30 por cento de SPPC em piso fixo e em esteira. MÉTODOS: Seis crianças com paralisia cerebral hemiplégica espástica (7,70±1,04 anos) foram filmadas andando com velocidade confortável sem o uso de SPPC, com 0 por cento e 30 por cento de SPPC em piso fixo e com 0 por cento e 30 por cento de SPPC em esteira. Marcadores refletivos foram afixados nos principais pontos anatômicos dos dois hemicorpos para registro das coordenadas "x", "y", "z". RESULTADOS: As crianças andaram mais rapidamente e com passadas mais longas e mais rápidas, com duração dos períodos de apoio simples e balanço maiores e apoio duplo menor no piso fixo do que na esteira, independentemente do uso do SPPC. O quadril foi a única articulação que apresentou diferenças entre os hemicorpos e entre as condições, sendo que o hemicorpo plégico apresentou menor amplitude de movimento (ADM) que o hemicorpo não plégico, e a ADM foi maior na condição sem o uso de SPPC do que com 30 por cento de SPPC em piso fixo. CONCLUSÃO: Crianças com paralisia cerebral hemiplégica espástica são capazes de andar em piso fixo e esteira com diferentes porcentagens de SPPC, sendo que seus desempenhos foram melhores no piso fixo, independentemente do uso de SPPC, do que na esteira.

OBJECTIVE: To analyze the spatial-temporal characteristics and joint angles during overground walking without body weight support (BWS) and with 0 percent and 30 percent BWS, and during treadmill walking with the same BWS in children with cerebral palsy. METHODS: Six children with hemiplegic and spastic cerebral palsy (7.70 ± 1.04 years old) were videotaped during overground walking at a comfortable speed with no BWS, with 0 percent and 30 percent BWS, and during treadmill walking with 0 percent and 30 percent BWS. Reflective markers were placed over main bony landmarks in both body sides to register the coordinates "x", "y", "z". RESULTS: During overground walking, children walked faster and presented longer and faster strides, longer duration of single-stance and swing periods, and shorter duration of double-stance period, than treadmill walking, regardless of BWS use. The hip was the only joint that presented a difference between body sides and experimental conditions; i.e. range of motion (ROM) was reduced in the plegic side when compared to the nonplegic side, and during overground walking without BWS when compared to 30 percent BWS. CONCLUSION: Children with hemiplegic and spastic cerebral palsy were able to walk overground and on a treadmill with different percentages of BWS, and their performance was superior during overground walking, regardless of BWS use.

Molecular characterization of Histoplasma capsulatum isolated from an outbreak in treasure hunters Histoplasma capsulatum in treasure hunters.

BACKGROUND: In Mexico, primary pulmonary histoplasmosis is the most relevant clinical form of the disease. The geographical distribution of specific strains of Histoplasma capsulatum circulating in Mexico has not been fully established. Outbreaks must be reported in order to have current, updated information on this disease, identifying new endemic areas, manner of exposure to the fungi, and molecular characterization of the causative agents. We report a recent outbreak of histoplasmosis in treasure hunters and the molecular characterization of two isolates obtained from these patients. METHODS: Six patients admitted to the National Institute of Respiratory Diseases (INER) in Mexico City presented severe respiratory symptoms suggestive of histoplasmosis. They acquired the infection in the Veracruz (VZ) endemic zone. Diagnosis was made by X-ray and Computed tomography (CT), liver function, immunological techniques, and culture. Identification of H. capsulatum isolates was confirmed by using Polymerase chain reaction (PCR) was conducted with a probe from the M antigen, and the isolates were characterized by means of Random amplification of polymorphic DNA (RAPD)-PCR employed the 1253 oligonucleotide and a mixture of oligonucleotides 1281 and 1283. These were compared to eight reference strain isolates from neighboring areas. RESULTS: X-ray and CT revealed disseminated micronodular images throughout lung parenchyma, as well as bilateral retrocaval, prevascular, subcarinal, and hilar adenopathies, hepatosplenomegaly, and altered liver function tests. Five of the six patients developed disseminated histoplasmosis. Two H. capsulatum strains were isolated. The same band profile was detected in both strains, indicating that both isolates corresponded to the sole H. capsulatum strain. Molecular characterization of the isolates was similar in 100% with the EH-53 Hidalgo human (HG) strain (reference strain integrated into the LAm A clade described for Latin America). CONCLUSIONS: The two isolates appeared to possess the same polymorphic pattern; they are indistinguishable from each other and from EH-53. It is important to remain updated on recent outbreaks of histoplasmosis, the manner of exposure to the fungi, as well as the molecular characterization of the isolates. The severity of cases indicates that this strain is highly virulent and that it is probably prevalent in Hidalgo and Veracruz states.

Clinical comparison of two Mexican coccidioidins.

Coccidioidin, an extract from the saprophytic mycelial form of Coccidioides spp., has been a very useful antigen preparation both for skin and serological tests for coccidioidomycosis. Unfortunately, coccidioidin is not currently available for skin testing in the United States. Coccidioidin has been produced commercially in Mexico by a vaccine and reagents laboratory of the Mexican Federal Government. It also has been produced at the Microbiology Laboratory of the Faculty of Medicine, Universidad Nacional Autónoma de México exclusively as an antigen for research projects. The objective of the study was to compare both coccidioidins in their reactivity and safety when applied in humans. One hundred and eighty-four volunteers were tested; median age was 33 (range 14-82). When the cutoff point is set in 5 mm, 88 subjects (47.8%) had a positive test for the commercial coccidioidin and 76 (41.3%; CI(95%) 0.50, 1.15; P = 0.20) were positive with the research antigen. Seventy-five subjects were positive for both antigens and 96 were negative for both. Fifty-nine subjects (31.3%) reported an adverse reaction after the application of the antigen; they were mostly very mild local reactions. Mexican research coccidioidin is a safe and reliable antigen that can be used for the detection of coccidioidomycosis infection in mammals.

Tratamiento con Amlodipina de la hipertensión arterial esencial leve/moderada / Treatment of the essencial arterial hypertension with Amlodipina

Se estudiaron 20 pacientes con el diagnóstico de hipertensión arterial esencial leve a moderada, con el objeto de investigar la eficacia y seguridad de la Amlodipina, como medicamento única o en combinación, en el tratamiento de la hipertensión esencial leve a moderada en la práctica clínica. Del total de 20 pacientes, 12 eran mujeres (60 por ciento ) y 8 hombres (40 por ciento ); la edad promedio fue de 51 años, con un peso medio de 66.26 Kg. Las cifras promedio de presión arterial al inicio del estudio fueron 164/103 mmHg. Se inició tratamiento con una dosis oral diaria de 5 mg de Amlodipina y al final de la sexta semana se realizó un ajuste de la dosis (10 mg/día) en nueve pacientes cuya presión arterial diastólica era mayor de 90 mmHg. Las cifras promedio de presión arterial a la décima semana fueron 151/88 mmHg, lo que representa un 100 por ciento de efectividad del medicamento en el control de la hipertensión arterial en el grupo de pacientes sujeto a estudio. Los efectos secundarios detectados durante el estudio fue un caso de edema de miembros inferiores leve, el cual se toleró sin necesidad de omitir el medicamento y que luego desapareció; los pacientes restantes tuvieron una excelente tolerancia a la droga