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1.
Food Chem Toxicol ; 150: 112066, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33596454

ABSTRACT

Neurodegenerative diseases are associated with chronic inflammatory states. There is evidence to support the design of novel supplements based on guarana (G) (Paullinia cupana), selenium (S), and L-carnitine (C), the use of which, potentially attenuates neuro oxi-inflammatory conditions. Therefore, this study analyzed the cytotoxic and redox effects of GSC on human leucocytes, the inflammatory activation of microglia BV-2 cells, and effect on mortality, oxidative metabolism, and the immune modulation of red earthworms (Eisenia fetida). The GSC concentrations tested in cell culture were in the range of 0.04-2.1 mg/mL. All the GSC-supplemented samples tested, reverted H2O2 oxidation in DNA molecules, suggesting its genoprotective potential. GSC did not induce mortality in leucocyte cultures. On the contrary, a reduction in the levels of oxidation of lipids, proteins, and cell apoptosis was observed, via downregulation of caspase 3 and 8 genes. GSC showed a dual effect on microglia, decreasing the cellular proliferation at lower concentrations (<0.24 mg/mL) and increasing the cellular proliferation mainly at concentrations > 1.0 mg/mL. GSC did not have a toxic effect on red earthworms, but induced an increase in amoebocyte cells and in brown body formation, indicating immune response activation. The results suggest that GSC could be safe for human consumption.


Subject(s)
Carnitine/pharmacology , Eimeria/drug effects , Paullinia , Selenium/pharmacology , Carnitine/chemistry , Cell Cycle , Cell Line , Cell Survival/drug effects , DNA Damage/drug effects , Humans , Lipid Peroxidation , Microglia , Oxidation-Reduction , Selenium/chemistry
2.
Chemosphere ; 251: 126642, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32345545

ABSTRACT

The ubiquitous presence of aluminum in the environment leads to a high likelihood of human exposure. Neurotoxicity of the trivalent cationic form of this metal (Al3+) occurs in the central nervous system via accumulation of Al in cells of neural origin, including neural progenitor cells (NPCs). NPCs play a key role in the development and regeneration of the brain throughout life; therefore, this metal may contribute to neuropathological conditions. Here, we evaluated the effects of different Al3+ concentrations (0-50 µM) on the purinergic system of NPCs isolated from embryonic telencephalons, cultured as neurospheres. Al3+ adhered to the cell surface of neurospheres reducing extracellular ATP release, as well as ATP, ADP, and AMP hydrolysis by NTPDase and 5'-nucleotidase, respectively. In addition, impaired nucleotide release by Al3+ reduced P2Y1 and adenosine A2A receptors expression in differentiated neurospheres. These receptors are crucial for NPC proliferation during brain development and self-repair against external stimuli, such as metal exposure. Thus, Al3+ represents an environmental agent linked to neurodegeneration through alterations in the ATP-signalling pathway, proving to be a potential mechanism associated with NPC proliferation and brain degeneration.


Subject(s)
Aluminum/toxicity , 5'-Nucleotidase , Adenosine Triphosphate/metabolism , Aluminum/metabolism , Animals , Cell Differentiation/drug effects , Cells, Cultured , Central Nervous System/metabolism , GPI-Linked Proteins , Humans , Signal Transduction/drug effects , Stem Cells , Toxicity Tests
3.
Arch Toxicol ; 93(9): 2515-2524, 2019 09.
Article in English | MEDLINE | ID: mdl-31363819

ABSTRACT

Aluminum (Al) is a neurotoxin and is associated with the etiology of neurodegenerative diseases, such as Alzheimer's disease (AD). The Al-free ion (Al3+) is the biologically reactive and toxic form. However, the underlying mechanisms of Al toxicity in the brain remain unclear. Here, we evaluated the effects of Al3+ (in the chloride form-AlCl3) at different concentrations (0.1-100 µM) on the morphology, proliferation, apoptosis, migration and differentiation of neural progenitor cells (NPCs) isolated from embryonic telencephalons, cultured as neurospheres. Our results reveal that Al3+ at 100 µM reduced the number and diameter of neurospheres. Cell cycle analysis showed that Al3+ had a decisive function in proliferation inhibition of NPCs during neural differentiation and induced apoptosis on neurospheres. In addition, 1 µM Al3+ resulted in deleterious effects on neural phenotype determination. Flow cytometry and immunocytochemistry analysis showed that Al3+ promoted a decrease in immature neuronal marker ß3-tubulin expression and an increase in co-expression of the NPC marker nestin and glial fibrillary acidic protein. Thus, our findings indicate that Al3+ caused cellular damage and reduced proliferation and migration, resulting in global inhibition of NPC differentiation and neurogenesis.


Subject(s)
Aluminum Chloride/toxicity , Embryonic Stem Cells/drug effects , Neural Stem Cells/drug effects , Neurogenesis/drug effects , Animals , Apoptosis/drug effects , Cell Cycle/drug effects , Cell Movement/drug effects , Cells, Cultured , Dose-Response Relationship, Drug , Embryonic Stem Cells/pathology , Female , Male , Mice , Neural Stem Cells/pathology , Neurotoxicity Syndromes/etiology , Neurotoxicity Syndromes/physiopathology , Phenotype , Telencephalon/drug effects , Telencephalon/embryology
4.
J Cell Biochem ; 119(7): 6249-6257, 2018 07.
Article in English | MEDLINE | ID: mdl-29663535

ABSTRACT

The purinergic system has an important role in the regulation of vascular functions. The interference of thyroid hormones in this system and in cardiovascular events has been studied in recent years. However, the mechanisms involved in vascular, purinergic, and oxidative changes in thyroid disorders are not completely understood. Therefore, the present study aimed to assess purinergic enzyme activity in platelets from rats with hypothyroidism and hyperthyroidism induced, respectively, by continuous exposure to methimazole (MMI) at 20 mg/100 mL or L-thyroxine at 1.2 mg/100 mL in drinking water for 1 month. Results showed that rats exposed to L-thyroxine had a significant decrease in NTPDase activity, wherein ATP hydrolysis was 53% lower and ADP hydrolysis was 40% lower. Moreover, ecto-5'-nucleotidase activity was decreased in both groups, by 39% in the hypothyroidism group and by 52% in the hyperthyroidism group. On the other hand, adenosine deaminase (ADA) activity was increased in hyperthyroidism (75%), and nucleotide pyrophosphatase/phosphodiesterase (NPP) activity was increased in animals with hypothyroidism (127%) and those with hyperthyroidism (128%). Our findings suggest that changes in purinergic enzyme and purine levels could contribute to the undesirable effects of thyroid disturbances. Moreover, oxidative stress and, in particular, a high level of ROS production, showed a causal relation with changes in ectonucleotidase activity and nucleotide and nucleoside levels.


Subject(s)
5'-Nucleotidase/metabolism , Adenosine Deaminase/metabolism , Antigens, CD/metabolism , Apyrase/metabolism , Blood Platelets/enzymology , Hyperthyroidism/enzymology , Hypothyroidism/enzymology , Nucleotides/metabolism , Adenosine Triphosphate/metabolism , Animals , Hydrolysis , Hyperthyroidism/blood , Hyperthyroidism/chemically induced , Hypothyroidism/blood , Hypothyroidism/chemically induced , Male , Methimazole/toxicity , Oxidative Stress , Rats , Rats, Wistar
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