ABSTRACT
BACKGROUND: To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model. METHODS: Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis. RESULTS: PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels. CONCLUSION: In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment.
Subject(s)
Anti-Infective Agents/pharmacology , Methylene Blue/pharmacology , Oxidative Stress/drug effects , Photosensitizing Agents/pharmacology , Animals , Anti-Infective Agents/therapeutic use , Ascorbic Acid/blood , Blood Vessels/drug effects , Blood Vessels/pathology , Blood Vessels/radiation effects , Dental Scaling , Gingiva/pathology , Gingiva/radiation effects , Glutathione/blood , Light , Male , Methylene Blue/therapeutic use , Oxidative Stress/radiation effects , Periodontitis/drug therapy , Periodontitis/radiotherapy , Photochemotherapy , Photosensitizing Agents/therapeutic use , Rats , Rats, Wistar , Root PlaningABSTRACT
This article presents the evolution and development that the Programmatic Area of Dissemination of Scientific and Technical Information of INCAP has had during the last five years. It therefore includes, objectives, activities conducted, and a description of achieved products, as well as short and medium-term perspectives foreseen.